Investigation of the effects of resveratrol on total oxidant/antioxidant capacity on experimental cigarette smoking model

Abstract

AIM The harmful effects of smoking on body are known to be caused by oxidative stress in tissues. With the many harmful substances in smoke it serve as a good model of free radical damage could be monitored. The agents preventing free radical induced oxidation, capable of trapping and stabilizing free radicals are called 'antioxidants". Resveratrol is a powerful antioxidant that has anti-inflammatory, analgesic and osteogenic effects. In this study we evaluated the effects of smoking and resveratrol on oxidative stress. MATERIAL VE METHOD For 4 weeks rats were inhalated cigarette smoke (6 cigarette/day) and after that time 20 mg/kg/day resveratrol was given via oral gavage during 28 days together with cigarette smoke. At the end of 8 weeks total exposure to cigarette smoke, rats were sacrified and bloods and tongue samples were collected. Biochemically serum and tissue total antioxidant / oxidant capacity (TAC / TOC) were evaluated. RESULTS There were no statistical difference between groups in terms of TAC and TOC serum. TAC tongue is higher in cigarette+resveratrol and cigarette groups. CONCLUSION In the experiment performed with 6 cigarettes per day for 8 weeks, toxic effect of cigarette induced antioxidant system in the organism to protect the body and that is more effective than antioxidant effect of resveratrol.

Keywords

• Smoking, resveratrol, total oxidant capacity, total antioxidant capacity

Deneysel sigara modelinde resveratrolün total oksidan/antioksidan kapasite üzerine etkilerinin araştırılması

Abstract

AMAÇ Sigaranın vücutta hasara neden olan etkilerinin dokularda oluşturduğu oksidatif strese bağlı olduğu bilinmektedir. Sigara içerdiği çok sayıdaki zararlı madde ile serbest radikal hasarının izlenebileceği bir model oluşturmaktadır. Serbest radikallerin neden olduğu oksidasyonları önleyen, serbest radikalleri yakalama ve stabilizasyon yeteneğine sahip maddelere 'antioksidan' adı verilir. Resveratrol antienflamatuar, osteojenik ve analjezik etkili güçlü bir antioksidandır. Bu çalışmada sigaranın neden olduğu oksidatif stres üzerine resvaratrolün etkisi araştırılmıştır. MATERYAL VE METOD Ratlar 4 hafta süresince sigara dumanına (6 sigara/gün) maruz bırakıldıktan sonra 28 gün boyunca 20 mg/kg/gün resveratrol intragastrik gavaj yoluyla verilmiştir. Sekiz haftalık deney süresi sonunda ratlar sakrifiye edilerek kan ve dil dokuları alınmıştır. Biyokimyasal olarak serum ve dokuda Total antioksidan/oksidan kapasite (TAK/TOK) değerlendirilmiştir. BULGULAR Serum TAK ve TOK açısından gruplar arasında anlamlı fark bulunmamıştır. TAK ve TOK dil sigara+resveratrol grubunda yüksek bulunmuştur. SONUÇ 8 hafta süresince günde 6 adet sigarayla yapılan deneyde, sigaranın toksik etkisine karşı vücudun kendini korumak için aktive olan antioksidan sistemin etkisinin, resveratrolün antioksidan kapasitesinden daha yüksek olduğu sonucuna varılmıştır.

Keywords

• Sigara, resveratrol, total oksidan kapasite, total antioksidan tapasite

References

1. Serafini M, Del Rio, D. Understanding the association between dietary antioxidants, redox status and disease: is the total antioxidant capacity the right tool. Redox Report 2004; 9: 145-152.
2. Delibaş N, Özcankaya R.Serbest radikaller. SDÜ Tıp Fakültesi Dergisi 1995; 2: 11-17.
3. Altuntaş I, Dane S, Gümüştekin K. Effects of cigarette smoking on lipid peroxidation. J Basic Clin Physiol Pharmacol 2002; 13: 69-72.
4. Pryor WA, Stone K. Oxidants in cigarette smoke. Radicals hydrogen peroxide, peroxynitrate, and peroxynitrite. Ann NY Acad Sci 1993; 686: 12-27.
5. Church DF, Pryor WA. Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect 1985; 64: 111-126.
6. Eiserich JP, Vossen V, O’Neill CA, Halliwell, B, Cross, CE, van der Vliet A. Molecular mechanism of damage by excess nitrogen oxides nitration of tyrosine by gas-phase cigarette smoke. FEBS Lett 1994; 353: 53-56.
7. Petruzzelli S, Puntoni R, Mimotti P, Pulerà N, Baliva F, Fornai E, Giuntini C. Plasma 3-nitrotyrosine in cigarette smokers. Am J Respir Crit Care Med 1997; 156: 1902-1907.
8. Elliot JG. Application of antioxidant vitamins in foods and beverages. Food Tech 1999; 53: 46-48.

9. Keskin D, Karsan O, Ezirmik N, Çiftçioğlu A. Tavşanlarda kırık iyileşmesi üzerine alfa-tokoferolün etkisi. Artroplasti- Artroskopik Cerrahi 1999; 10: 207-210. grubunda, dil ty of resveratrol and alcohol-free wine polyphenols related to
10. LDL oxidation and polyunsaturated fatty acids. Life Sci 1999; 64: 2511-2521.
11. Erel O. A new automated colorimetric method for measur- ing total oxidant status. Clin Biochem 2005; 38: 1103-1111.
12. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem 2004; 37: 277-285.
13. Halliwell B, Aruoma OR. DNA damage by oxygen de- rived species. Its mechanisms of action and measurements in mammalian system. FEBS Lett 1991; 281: 9-19.
14. Bruno RS, Traber MG. Vitamin E biokinetics, oxidative stress and cigarette smoking. Pathophysiology 2006; 13: 143
15. Kalra J, Chaudhary AK, Prasad K. Increased production of oxygen free radicals in cigarette smokers. Int J Exp Pathol 199 ; 72: 1-7. Kodama M, Kaneko M, Aida M, Inoue F, Nakayama T, Akimoto H. Free radical chemistry of cigarette smoke and its implication in human cancer. Anticancer Res 1997; 17: 433- 4
16. Jannin B, Menzel M, Berlot JP, Delmas D, Lançon A, La- truffe N. Transport of resveratrol, a cancer chemopreventive agent, to cellular targets: Plasmatic protein binding and cell uptake. Biochem Pharmacol 2004; 68: 1113-1118.
17. Falchetti R, Fuggetta MP, Lanzilli G, Tricarico M, Ravag- nan G. Effects of resveratrol on human immune cell function. Life Science 2001; 70: 81-96.
18. Pedras MSC, Ahiahonu PWK. Metabolism and detoxifi- cation of phytoalexins and analogs by phytopathogenic fungi. Phytochemistry 2005; 66: 391–411.
19. Göktaş Ö. Kadmiyumun neden olduğu oksidatif stres üzerine resveratrolün koruyucu etkisi. Yüksek Lisans Tezi, Malatya, (Prof. Dr. Göknur Aktay), 2007 https://tez.yok.gov. tr/UlusalTezMerkezi/TezGoster. (17.01.2014).
20. Psotova J, Zahalkova J, Hrbac J, Simanek V, Bartek J. De- termination of total antioxidant capacity in plasma by cyclic voltammetry. Biomed Papers 2001; 145: 81-83. van Rossum JP, Schamhart DH. Oxidation-reduction (re- dox) potentiometry in blood in geriatric conditions: a pilot study. Exp Gerontol 1991; 26: 37-43.
21. Prior RL, Cao G. In vivo total antioxidant capacity: com- parison of different analytical methods. Free Radic Biol Med 1999; 27: 1173-1181.