İyonize Radyasyonun Neden Olduğu Ovaryum Hasarına Karşı Curcuminin Koruyucu Etkisinin Morfolojik ve İmmünohistokimyasal Olarak İncelenmesi

Yıl 2018, , 102 - 111, 01.08.2018

Kanat Gülle , İbrahim Pala Meryem Akpolat , Bekir Hakan Bakkal

https://doi.org/10.22312/sdusbed.301632

Öz

Çalışmamızda,
iyonize radyasyonun folliküler atrezi üzerine etkilerini göstererek, oluşacak
hasarlara karşı curcuminin koruyucu etkisinin olup olmadığını ve hücre
çekirdeklerinde meydana gelen DNA hasarını tamir eden Poli
(ADP-riboz)polimeraz-1 enziminin immünohistokimyasal boyama yöntemiyle
gösterilmesi amaçlandı.

            Çalışmamız için seçilen dişi
sıçanlar, biri kontrol olmak üzere toplam 4 gruba ayrıldı. Radyasyon hasarı
oluşturmak amacıyla kontrol ve curcumin grubu dışındaki deneklere tek doz tüm
vücut 8.3 Gy iyonize radyasyon uygulandı. Curcumin ve radyasyon+curcumin grubu
deneklere; ışınlamadan 7 gün önce başlayarak günde 100 mg/kg curcumin oral
yoldan verildi. Işınlamadan sonraki 4. günde deneklerden anestezi altında
ovaryum dokuları alınarak mikroskobik incelemeler için işlemlendirildi.

            Radyasyon uygulamasından 4 gün sonra
alınan ovaryum dokularının kesitlerini incelediğimizde, ışınlanmış gruplardaki
atretik özellik gösteren folliküller sayıca fazlaydı. Radyasyon ile ışınlanan
curcumin tedavili grupta ise atretik follikül oranı radyasyon grubuna göre daha
düşüktü. Curcumin ile tedavi edilen grupta, curcuminin radyasyona bağlı meydana
gelen hasarları kısmen engellediği tespit edildi.

Anahtar Kelimeler

İyonize radyasyon, Follikülogenezis, Curcumin, PARP1

Kaynakça

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