Oxytocin Effect on Sepsis-induced Experimental Rat Renal Failure Model: A Histological and Biochemical study

Abstract

We aimed to investigate the therapeutic role of Oxytocin (OT) in sepsis induced rats and its effects on kidneys. Sepsis was induced in 24 of them by cecal ligation-perforation (CLP) method. One of the groups of CLP did not recieve any treatment while either saline 1 ml/kg or OT 0.4 mg/kg was applied intraperitoneally to the other groups within the first hour of surgery. 8 rats were sham-operated and 8 rats were spared as control group. Plasma TNF-α, CRP, MDA levels, total antioxidant capacity (TAC), creatinine level and peripheral leukocyte (WBC) counts were measured. TNF-α, CRP, MDA levels and WBC counts were significantly increased in CLP and CLP-saline groups, on the contrary they are decreased in CLP-OT group. Histopathologically loss of brush border, tubular dilatation and mononuclear cell infiltration was significantly decreased in CLP-OT group but there was no significant difference in erytrocyte extravasation and cast formation when compared to CLP and CLP-saline groups. Our results indicate that OT may have a therapeutic value in limiting sepsis induced inflammation and organ damage.

Keywords

• Sepsis model,Kidney,Oxytocin

Sepsis Kaynaklı Deneysel Sıçan Böbrek Hasarı Modeli Üzerine Oksitosin Etkisi: Histolojik ve Biyokimyasal Bir Çalışma

Abstract

Bu çalışmada, sepsis ile indüklenen sıçanlarda oksitosinin (OT) terapatik rolü ve bunun böbrekler üzerindeki etkisi araştırılmıştır. 24 adet sıçanda çekal ligasyon-perforasyon (CLP) yöntemi ile sepsis oluşturulmuştur. Cerrahi girişimin ilk saati içerisinde, CLP oluşturulan gruplarından birine herhangi bir tedavi uygulanmamış diğer gruba 1 ml/kg salin ve 0.4 mg/kg OT intraperitoneal olarak uygulanmıştır. 8 sıçan sham opere, 8 sıçan da kontrol grubu olarak ayrılmıştır. Plazmada TNF-α, CRP, MDA düzeyleri, toplam antioksidan kapasite (TAK), kreatinin seviyesi ve periferik lökosit (WBC) sayıları ölçülmüştür. CLP ve CLP-salin gruplarında TNF-α, CRP, MDA düzeyleri ve WBC sayılarında anlamlı artma saptanırken CLP-OT grubunda anlamlı düşüş görülmüştür. CLP ve CLP-salin gruplarıyla kıyaslandığında; CLP-OT grubunda serum MDA, CRP ve WBC seviyeleri anlamlı olarak düşerken, TAC anlamlı derecede yüksek çıkmıştır. CLP-OT gruplarında, histopatolojik olarak fırçamsı kenar kaybı, tübüler dilatasyon ve mononükleer hücre infiltrasyonu anlamlı düzeyde azalmıştır. Ancak, CLP ve CLP-salin grupları karşılaştırıldığında ekstravazasyon ve kast bulguları açısından anlamlı fark bulunamamıştır. Sonuçlar, OT'nin sepsis kaynaklı inflamasyon ve organ hasarı açısından terapotik değeri olabileceğini göstermektedir.

Keywords

• Sepsis modeli,Böbrek,oksitosin

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