Oxytocin Effect on Sepsis-induced Experimental Rat Renal Failure Model: A Histological and Biochemical study

Yıl 2018, , 28 - 33, 17.04.2018

Turker Çavuşoğlu Yusuf Savran Yiğit Uyanıkgil , Kanat Gülle , Ahmet Çağdaş Acara Gürkan Yiğittürk Kubilay Doğan Kılıç Emel Öykü Çetin Ayfer Meral Oytun Erbaş

https://doi.org/10.22312/sdusbed.305491

Cited By: 1

Öz

We aimed to investigate the therapeutic role of Oxytocin
(OT) in sepsis induced rats and its effects on kidneys. Sepsis was induced in 24 of them by cecal ligation-perforation
(CLP) method. One of the groups of CLP did not recieve any treatment while
either saline 1 ml/kg or OT 0.4 mg/kg was applied intraperitoneally to the
other groups within the first hour of surgery. 8 rats were sham-operated and 8
rats were spared as control group. Plasma TNF-α, CRP, MDA levels, total
antioxidant capacity (TAC), creatinine level and peripheral leukocyte (WBC)
counts were measured. TNF-α, CRP, MDA levels and WBC counts were significantly
increased in CLP and CLP-saline groups, on the contrary they are decreased in
CLP-OT group. Histopathologically loss of brush border, tubular dilatation and
mononuclear cell infiltration was significantly decreased in CLP-OT group but
there was no significant difference in erytrocyte extravasation and cast
formation when compared to CLP and CLP-saline groups. Our results indicate that
OT may have a therapeutic value in limiting sepsis induced inflammation and
organ damage.

Anahtar Kelimeler

Sepsis model, Kidney, Oxytocin

Sepsis Kaynaklı Deneysel Sıçan Böbrek Hasarı Modeli Üzerine Oksitosin Etkisi: Histolojik ve Biyokimyasal Bir Çalışma

Öz

Bu çalışmada, sepsis ile indüklenen sıçanlarda
oksitosinin (OT) terapatik rolü ve bunun böbrekler üzerindeki etkisi
araştırılmıştır. 24 adet sıçanda çekal ligasyon-perforasyon (CLP) yöntemi ile
sepsis oluşturulmuştur. Cerrahi girişimin ilk saati içerisinde, CLP oluşturulan
gruplarından birine herhangi bir tedavi uygulanmamış diğer gruba 1 ml/kg salin
ve 0.4 mg/kg OT intraperitoneal olarak uygulanmıştır. 8 sıçan sham opere, 8
sıçan da kontrol grubu olarak ayrılmıştır. Plazmada TNF-α, CRP, MDA düzeyleri,
toplam antioksidan kapasite (TAK), kreatinin seviyesi ve periferik lökosit
(WBC) sayıları ölçülmüştür. CLP ve CLP-salin gruplarında TNF-α, CRP, MDA
düzeyleri ve WBC sayılarında anlamlı artma saptanırken CLP-OT grubunda anlamlı
düşüş görülmüştür. CLP ve CLP-salin gruplarıyla kıyaslandığında; CLP-OT
grubunda serum MDA, CRP ve WBC seviyeleri anlamlı olarak düşerken, TAC anlamlı
derecede yüksek çıkmıştır. CLP-OT gruplarında, histopatolojik olarak fırçamsı
kenar kaybı, tübüler dilatasyon ve mononükleer hücre infiltrasyonu anlamlı
düzeyde azalmıştır. Ancak, CLP ve CLP-salin grupları karşılaştırıldığında
ekstravazasyon ve kast bulguları açısından anlamlı fark bulunamamıştır.
Sonuçlar, OT'nin sepsis kaynaklı inflamasyon ve organ hasarı açısından terapotik
değeri olabileceğini göstermektedir.

Anahtar Kelimeler

Sepsis modeli, Böbrek, oksitosin

Kaynakça

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