Investigation of Androgen Responsive Elements in Some Autophagy Related Genes via In Silico Analysis

Abstract

Amaç: Otofaji, hasarlı organellerin, hatalı katlanmış veya uzun ömürlü proteinlerin, ökaryotik hücrelerde lizozom aracılı uzaklaştırılmasından sorumlu olan majör bir hücresel yoldur ve mayadan memelilere kadar iyi düzeyde korunmuş fizyolojik mekanizmadır. Otofaji, nörodejeneratif hastalıklar, bulaşıcı hastalıklar, metabolik hastalıklar ve kanserinde dahil olduğu çok çeşitli patolojilerle ilişkilendirilmiştir. Son çalışmalar otofajinin prostat kanserindeki rolünü vurgulamaktadır. Bu nedenle, otofajinin bilinmeyen mekanizmalar ile gerçekleşen regülasyonu ve özellikle androjen aracılı gerçekleşen regülasyonu hakkındaki bilinmeyen süreçlerin anlaşılması önem taşımaktadır. Bu çalışmada otofaji ile ilişkili genlerin promotor bölgesinde in silico analizler ile varsayılan ARE’leri araştırmayı amaçladık. Materyal-Metod: Otofaji ile ilişkili hedef genlerin promotör dizileri UCSC ve EPD veri tabanından ekstrakte edilerek, androjen resepötörü bağlanma motifi için varsayılan bağlanma bölgeleri matinspector biyoinformatik aracı ile analiz edildi.

Bulgular: İn silico analiz sonuçlara göre otofaji ile ilişkili 33 farklı genin promotor bölgesinde varsayılan ARE’ler tanımlandı.

Sonuç: Sonuçlarımız otofaji ile ilişkili komponentlerin androjen sinyalizasyonu ile sıkı şekilde düzenlenebileceğini önermektedir.

Keywords

• Otofaji,Androjen cevap elementleri,Prostat kanseri,in silico analiz

Investigation of Androgen Responsive Elements in Some Autophagy Related Genes via in silico Analysis

Abstract

Objective: Autophagy is a major cellular pathway that is responsible for removal of damaged organelles, misfolded or long-lived proteins by lysosome in eukaryotic cells, and it is a well conserved physiological mechanism from the yeast to mammalian. Autophagy has been associated with several pathologies, including neurodegenerative diseases, infectious diseases, metabolic diseases and cancer. Recent studies highlight the role of autophagy in prostate cancer. Thus, it is important to understand the unknown processes about regulation of autophagy with its different mechanisms, especially androgen mediated regulation. In this study, our aim was to investigate the putative AREs in promoter sequence of autophagy related genes by in silico analysis. Material-Method: Promoter sequences of the autophagy related target genes extracted from UCSC and EPD databases and then analyzed putative binding sites for androgen receptor binding motif by matinspector bioinformatics tool. Results: The result of the in silico analysis indicates that putative AREs in the promoter region of 33 different genes associated with autophagy are identified. Conclusions: Our data the positive correlation between suggest that autophagy related components may be tightly regulated by androgen signaling.

Keywords

• Autophagy,androgen responsive elements (AREs),Prostate cancer,İn silico analysis

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