Deney Hayvanlarında Oluşturulan Periapikal Lezyon Modelinde Selenyumun Kandaki Oksidatif Stres Parametreleri Üzerine Etkileri

Abstract

Amaç: Bu çalışmanın amacı selenyumun ratlarda deneysel olarak indüklenen periapikal lezyon varlığında kan inflamasyon ve oksidatif stres belirteçleri üzerine etkilerinin araştırılmasıdır.

Gereç ve Yöntem: Bu çalışmada 30 adet erişkin Sprague-Dawley cinsi erkek rat rastgele negatif kontrol, pozitif kontrol ve selenyum grubu olmak üzere 3 gruba ayrıldı. Pozitif kontrol ve selenyum grubundaki ratlarda alt çene 1. azı dişlerinde periapikal lezyon oluşumu tetiklendi. Selenyum grubundaki ratlara deney süresince (28 gün) periton içine selenyum uygulandı. Yirmi sekinci günde alınan kan örneklerinde IL-6, total oksidan (TOS), total antioksidan (TAS) kapasite, oksidatif stres indeksi (OSİ), iskemi modifiye albümin (İMA), natif tiyol (NT) ve total tiyol (TT) seviyeleri değerlendirildi.

Bulgular: Gruplar arasında serum IL-6 seviyesi açısından istatistiksel olarak anlamlı fark tespit edildi (p<0,01). Selenyum grubundaki IL-6 seviyesi pozitif kontrol grubundan daha düşüktü (p<0,01). Gruplar arasında TAS, TOS ve OSİ seviyeleri açısından istatistiksel olarak anlamlı farklılık gözlendi (p<0,01). Gruplar arasında en yüksek serum TAS seviyesi negatif kontrol grubunda tespit edilirken, selenyum grubu ile aralarında istatistiksel bir fark bulunmadı. Gruplar arasında en yüksek OSİ değeri pozitif kontrol grubunda tespit edildi (p<0,05). OSİ değeri selenyum grubunda pozitif kontrol grubuna göre anlamlı derecede düşüktü (p<0,01). Gruplar arasında en yüksek TT ve NT değerleri selenyum grubunda tespit edildi (p<0,01). Gruplar arası karşılaştırmalarda İMA ve Disülfid değerleri bakımından istatistiksel olarak anlamlı bir fark saptanmadı (p>0,05).

Sonuç: Antioksidan enzim sentezini artırdığı bilinen selenyumun periapikal lezyon gibi lokal kronik enfeksiyonlar sonucu kanda meydana gelen oksidan ve enflamatuvar parametrelerin artışına karşı koruyucu rol oynayarak sistemik sağlığın devam ettirilmesinde etkili olacağı düşünülmektedir. 

Keywords

• Periapikal lezyon
• oksidatif stres
• selenyum
• total oksidan kapasite
• total antioksidan kapasite

The Effects of Selenıum on The Blood Oxıdatıve Stress Parametres in The Perıapıcal Lesıon Whıch Was Formed on Rats

Abstract

Objective: To investigate the effects of selenium against blood serum oxidative stress and inflammation markers after periapical lesion induction.

Material and Method: In this study, 30 adult male Sprague-Dawley rats were randomly divided into negative control, positive control and selenium groups (n=10). Periapical lesion was induced in mandibular first molars in the positive control and selenium groups. Rats in selenium group were applied to intraperitoneal selenium during 28 days. The blood taken on the day 28 was homogenized and was used in the analysis of the IL-6, total oxidant capacity (TOS), total antioxidant capacity (TAS), oxidative stress index (OSI), ischemia modified albumin (IMA), native thiol (NT) and total thiol (TT) parameters.

Results: Statistically significant difference was determined among the groups in terms of serum IL-6 level (p<0.01). The lowest serum IL-6 level was detected in the negative control group (p<0.01). Statistically significant differences were observed among the groups in terms of TAS, TOS and OSI levels. While the highest serum TAS level was in the negative control group, there was no statistical difference between the selenium and negative control groups. The highest TT and NT values among the groups were detected in the selenium group (p<0.01), and there was no statistical difference between the negative and positive control groups in terms of NT value (p=0.052). No statistically significant difference was found in terms of IMA and Disulfide values among groups (p>0.05).

Conclusions: Selenium, which is known to increase antioxidant enzyme synthesis, is thought to be effective in maintaining systemic health by playing a protective role against the increase in oxidant and inflammatory parameters in the blood as a result of local chronic infections. 

Keywords

• Periapical Lesion
• Periapical lesion
• oxidative stress
• selenium
• total antioxidant status
• total oxidant status

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