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Endodontik Çalışmalarda Deney Hayvanlarının Kullanımı ve Seçim Kriterleri

Year 2013, Volume: 4 Issue: 2, 81 - 90, 21.08.2013

Abstract

Hayvan deneyleri, hayvanların bilimsel çalışmalarda kullanılması olarak tanımlanır. Bu deneyler insanlar ve hayvanlarda var olan hastalıklar hakkında bilgi edinilmesinde, yeni bir madde veya yeni bir cerrahi tekniğin test edilmesinde bilim adamlarına yardımcı olmaktadır. Hayvan modelleri endodontide önemli bir yer tutmaktadır. Bu derlemede, bir hayvan çalışması planlarken hayvan modelinin seçimi ve çalışmalarda etik prensiplerin uygulanması ele alınmıştır. Sonuç olarak endodontide kök hücre çalışmaları dışındaki dışındaki bütün çalışmalar fare, rat ve dağ gelincikleri üzerinde yapılabilir.

References

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  • Fouad A, Barry J, Russo J, Radolf J, Zhu Q. Periapical lesion progression with controlled microbial inoculation in a type I diabetic mouse model. J Endod 2002; 28 (1): 8–16.
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  • Faria G, Celes MR, De Rossi A, Silva LA, Silva JS, Rossi MA. Evaluation of chlorhexidine toxicity injected in the paw of mice and added to cultured I929 fibroblasts. J Endod 2007;33(6):715–722
  • Rezende TM, Vieira LQ, Sobrinho AP, Oliveira RR, Taubman MA, Kawai T. The influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice. J Endod 2008; 34 (9): 1066–1071.
  • Mutoh N, Watabe H, Chieda K, Tani–Ishii N. Expression of Toll–like receptor 2 and 4 in inflamed pulp in severe combined immunodeficiency mice. J Endod 2009; 35 (7): 975–980.
  • Alshwaimi E, Purcell P, Kawai T, Sasaki H, Oukka M, Campos–Neto A, Stashenko P. Regulatory T cells in mouse periapical lesions. J Endod 2009; 35 (9): 1229–1233.
  • Metzger Z, Lin YY, Dimeo F, Ambrose WW, Trope M, Arnold RR. Synergistic pathogenicity of Porphyromonas gingivalis and Fusobacterium nucleatum in the mouse subcutaneous chamber model. J Endod 2009; 35 (1): 86–94.
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  • Garcia Lda F, Marques AA, Roselino Lde M, Pires– de–Souza Fde C, Consani S. Biocompatibility evaluation of Epiphany/Resilon root canal filling system in subcutaneous tissue of rats. J Endod 2010; 36 (1): 110–114.
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  • da Silva LA, Nelson–Filho P, da Silva RA. Revascularization and periapical repair after endodontic treatment using apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing in dogs teeth with apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109 (5): 779–787.
  • Wang X, Thibodeau B, Trope M, Lin L, Huang GT. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod 2010; 36 (1): 56–63.
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  • Garcia Lda F, Marques AA, Roselino Lde M, Pires– de–Souza Fde C, Consani S. Biocompatibility evaluation of Epiphany/Resilon root canal filling system in subcutaneous tissue of rats. J Endod 2010; 36 (1): 110–114.
  • Gomes–Filho JE, de Moraes Costa MM, Cintra LT, Duarte PC, Takamiya AS, Lodi CS, Bernabé PF. Evaluation of rat alveolar bone response to Angelus MTA or experimental light–cured mineral trioxide aggregate using fluorochromes. J Endod 2011; 37 (2): 250–254.
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  • Wang X, Thibodeau B, Trope M, Lin LM, Huang GT. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod 2010; 36 (1): 56–63.
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  • Dissanayaka WL, Zhu X, Zhang C, Jin L. Characterization of dental pulp stem cells isolated from canine premolars. J Endod 2011; 37 (8): 1074– 1080.
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Using and Selection Criterias of Laboratory Animals with Experimental Purpose in Endodontics

Year 2013, Volume: 4 Issue: 2, 81 - 90, 21.08.2013

Abstract

Animal experimentation is the use of animals in scientific research. Animal experimants help scientists understand diseases that exist animals and humans, for example new medicines oe new surgical techniques. Animal models have an significiant place in endodontics. Planning the animal experiments, choosing the animal models and the applying ethical principles, in researchs have been included in this review. We concluded that the animal experimants could be conducted on rats, mices and ferrets except the stem cell researches which must be done on dogs as an animal model in endodontics.

References

  • Başaran A.Deney Hayvanları. 1inci baskı.48, Nisan Kitabevi, Eskişehir, 2003.
  • Baumgartner JC, Falkler WJ, Beckerman T. Experimentally induced infection by oral anaerobic microorganisms in a mouse model. Oral Microbiol Immunol 1992; 7(4):253–256.
  • Feuille F, Ebersole JL, Kesavalu L, Stepfen MJ, Holt SC. Mixed infection with Porphyromonas gingivalis and Fusobacterium nucleatum in a murine lesion model. potential synergistic effects on virulence, Infect Immun 1996; 64 (6): 2094–2100.
  • Sobrinho AP, Barros MH, Nicoli JR, Carvalho MA, Farias LM, Bambirra EA, Bahia MG, Vieira EC. Experimental root canal infections in conventional and germ–free mice. J Endod 1998; 24 (6): 405–408.
  • Atkinson MA, Leiter EH. The NOD mouse model of type I diabetes: as good as it gets?. Nature Med. 1999; 5 (6): 601–604.
  • Fouad A, Barry J, Russo J, Radolf J, Zhu Q. Periapical lesion progression with controlled microbial inoculation in a type I diabetic mouse model. J Endod 2002; 28 (1): 8–16.
  • Moretton TR, Brown CE Jr, Legan JJ, Kafrawy AH. Periapical Lesion Progression with Controlled Microbial Inoculation in a Type I Diabetic Mouse Model. J Biomed Mater Res 2000; 52 (3): 528–533.
  • de Melo Maltos SM, Ribeiro Sobrinho AP, Silva FV, Nicoli JR, Roque de Carvalho MA, Vieira LQ, de Macêdo Farias L. Bacterial concentrations determine the ability to implant in the root canal system and translocate to lymph nodes in germ–free mice. J Endod 2003; 29 (1): 24–27.
  • Faria G, Celes MR, De Rossi A, Silva LA, Silva JS, Rossi MA. Evaluation of chlorhexidine toxicity injected in the paw of mice and added to cultured I929 fibroblasts. J Endod 2007;33(6):715–722
  • Rezende TM, Vieira LQ, Sobrinho AP, Oliveira RR, Taubman MA, Kawai T. The influence of mineral trioxide aggregate on adaptive immune responses to endodontic pathogens in mice. J Endod 2008; 34 (9): 1066–1071.
  • Mutoh N, Watabe H, Chieda K, Tani–Ishii N. Expression of Toll–like receptor 2 and 4 in inflamed pulp in severe combined immunodeficiency mice. J Endod 2009; 35 (7): 975–980.
  • Alshwaimi E, Purcell P, Kawai T, Sasaki H, Oukka M, Campos–Neto A, Stashenko P. Regulatory T cells in mouse periapical lesions. J Endod 2009; 35 (9): 1229–1233.
  • Metzger Z, Lin YY, Dimeo F, Ambrose WW, Trope M, Arnold RR. Synergistic pathogenicity of Porphyromonas gingivalis and Fusobacterium nucleatum in the mouse subcutaneous chamber model. J Endod 2009; 35 (1): 86–94.
  • Garlet TP, Fukada SY, Saconato IF, Avila–Campos MJ, da Silva TA, Garlet GP, Cunha Fde Q. CCR2 deficiency results in increased osteolysis in experimental periapical lesions in mice. J Endod 2010; 36 (2): 244–250.
  • Flake NM, Zweifel LS. Behavioral effects of pulp exposure in mice lacking cannabinoid receptor 2. J Endod 2012; 38 (1): 86–90.
  • Zortuk M, Kıraç E. Laboratuvar hayvanlarının deneysel amaçla protetik diş tedavisi alanında kullanım prosedürleri. Erciyes Univ Sağlık Bilim Derg 2010; 19 (3): 226–231.
  • Garcia Lda F, Marques AA, Roselino Lde M, Pires– de–Souza Fde C, Consani S. Biocompatibility evaluation of Epiphany/Resilon root canal filling system in subcutaneous tissue of rats. J Endod 2010; 36 (1): 110–114.
  • Huxley HG. The histology of rat molar tooth fissure plaque. Arch Oral Biol 1971; 16 (11): 1311–1328.
  • Stashenko P, Wang CY, Tani–ishii N, Yu SM. Pathogenesis of induced rat periapical lesions. Oral Surg Oral Med Oral Pathol 1994, 78 (4): 494–502.
  • Muruzabal M, Eurasquin J. Discussion of methods and criteria in evaluation of periapical response. Int Dent J 1970, 20 (3): 539–554.
  • Dammaschke T. Rat molar teeth as a study model for direct pulp capping research in dentistry. Lab Anim 2010; 44 (1): 1–6.
  • Guo W, Gong K, Shi H, Zhu G, He Y, Ding B, Wen L, Jin Y. Dental follicle cells and treated dentin matrix scaffold for tissue engineering the tooth root. Biomaterials 2012; 33 (5): 1291–1302.
  • Oikawa T, Nomura Y, Arai C, Noda K, Hanada N, Nakamura Y. Mechanism of active eruption of molars in adolescent rats. Eur J Orthod 2011; 33 (3): 221– 227.
  • Duarte PM, Tezolin KR, Figueiredo MF, Feres M, Bastos MF. Microbial profile of ligature–induced periodontitis in rats. Arch Oral Biol. 2010; 55 (2): 142–147.
  • Nakasone N, Yoshie H, Ohshima H. An immunohistohemical study of the expression of heat– shock protein–25 and cell proliferation in the dental pulp and enamel organ during odontogenesis in rat molars. Arch Oral Biol 2006; 51 (5): 378–386.
  • Thibodeau B, Teixeira F, Yamauchi M, Caplan DJ, Trope M. Pulp revascularization of immature dog teeth with apical periodontitis. J Endod 2007; 33 (6): 680–689.
  • da Silva LA, Nelson–Filho P, da Silva RA. Revascularization and periapical repair after endodontic treatment using apical negative pressure irrigation versus conventional irrigation plus triantibiotic intracanal dressing in dogs teeth with apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109 (5): 779–787.
  • Wang X, Thibodeau B, Trope M, Lin L, Huang GT. Histologic characterization of regenerated tissues in canal space after the revitalization/revascularization procedure of immature dog teeth with apical periodontitis. J Endod 2010; 36 (1): 56–63.
  • Metzger Z, Klein H, Klein A, Tagger M. Periapical lesion development in rats inhibited by dexamethasone. J Endod 2002; 28 (9): 643–645.
  • Nakamura K, Yamasaki M, Nishigaki N, Iwama A, Imaizumi I, Nakamura H, Kameyama Y. Effect of methotrexate–induced neutropenia on pulpal inflammation in rats. J Endod 2002; 28 (4): 287–290.
  • Johnson RB. Effects of multiple dentinal lesions on the rat pulp. J Endod 2004; 30 (12): 868–871.
  • Xiong H, Peng B, Wei L, Zhang X, Wang L. Effect of an estrogen–deficient state and alendronate therapy on bone loss resulting from experimental periapical lesions in rats. J Endod 2007; 33 (11): 1304–1308.
  • Morimoto T, Yamasaki M, Nakata K, Tsuji M, Nakamura H. The expression of macrophage and neutrophil elastases in rat periradicular lesions. J Endod 2008; 34 (9): 1072–1076.
  • Garber SE, Shabahang S, Escher AP, Torabinejad M. The effect of hyperglycemia on pulpal healing in rats. J Endod 2009; 35 (1): 60–62.
  • Lee YL, Hong CY, Kok SH, Hou KL, Lin YT, Chen MH, Wang CC, Lin SK. An extract of green tea, epigallocatechin–3–gallate, reduces periapical lesions by inhibiting cysteine–rich 61 expression in osteoblasts. J Endod 2009; 35 (2): 206–211.
  • Garcia Lda F, Marques AA, Roselino Lde M, Pires– de–Souza Fde C, Consani S. Biocompatibility evaluation of Epiphany/Resilon root canal filling system in subcutaneous tissue of rats. J Endod 2010; 36 (1): 110–114.
  • Gomes–Filho JE, de Moraes Costa MM, Cintra LT, Duarte PC, Takamiya AS, Lodi CS, Bernabé PF. Evaluation of rat alveolar bone response to Angelus MTA or experimental light–cured mineral trioxide aggregate using fluorochromes. J Endod 2011; 37 (2): 250–254.
  • Scarparo RK, Dondoni L, Böttcher DE, Grecca FS, Rockenbach MI, Batista EL Jr. Response to intracanal medication in immature teeth with pulp necrosis: an experimental model in rat molars. J Endod 2011; 37 (8): 1069–1073.
  • Fouad AF, Walton RE, Rittman BR. Healing of induced periapical lesions in ferret canines. J Endod 1993; 19 (3): 123–129.
  • Torabinejad M, Corr R, Buhrley M, Wright K, Shabahang S. An animal model to study regenerative endodontics. J Endod 2011; 37 (2): 197–202.
  • Holland GR. Periapical response to apical plugs of dentin and calcium hydroxide in ferret canines. J Endod 1984; 10 (2): 71–74.
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There are 90 citations in total.

Details

Primary Language Turkish
Journal Section Derlemeler
Authors

İhsan Ertuğrul

Murat Maden This is me

Ekim Orhan

Publication Date August 21, 2013
Submission Date September 27, 2012
Published in Issue Year 2013 Volume: 4 Issue: 2

Cite

Vancouver Ertuğrul İ, Maden M, Orhan E. Endodontik Çalışmalarda Deney Hayvanlarının Kullanımı ve Seçim Kriterleri. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi. 2013;4(2):81-90.

SDÜ Sağlık Bilimleri Dergisi, makalenin gönderilmesi ve yayınlanması dahil olmak üzere hiçbir aşamada herhangi bir ücret talep etmemektedir. Dergimiz, bilimsel araştırmaları okuyucuya ücretsiz sunmanın bilginin küresel paylaşımını artıracağı ilkesini benimseyerek, içeriğine anında açık erişim sağlamaktadır.