Genotoxic and Apoptotic effects of Heavy Metal Mixture on Human Aortic Vascular Smooth Muscle Cell Line

Yıl 2019, Cilt: 10 Sayı: 3, 237 - 243, 20.09.2019

Emrah Yavuz Zeynep Banu Doğanlar

https://doi.org/10.22312/sdusbed.513022

Cited By: 1

Öz

Objective: Heavy metals are the most
common pollutants with specific gravity greater than 5 g cm^-3. Human
exposure to heavy metals occurs mostly through the consumption of food and
water containing heavy metals. Metals that enter the body via drinking water
may cause genotoxicity and various diseases. Therefore, permissible limits of
heavy metals in water for human consumption have been determined by laws and
regulations. Our study aimed to investigate the effects of a mix of metals (Cu,
Zn, Pb and Fe) at permissible limits in drinking water on aortic smooth muscle
cells (TG-HA-VSMC) with at molecular level.

Material-Method: A metal mix at the
permissible concentration for drinking water and 10-fold higher concentration
were exposed to human aortic smooth muscle for 24 and 48 hours, and gene
expression of antioxidant enzymes, heat-shock proteins and apoptosis pathway
were investigated in addition to RAPD-DNA polymorphism.

Results: It was determined that heavy
metal exposure caused increase in the DNA polymorphism, apoptotic cell ratios
and enhanced antioxidant and apoptosis pathway gene expression in cells.

Conclusion: In conclusion, the mix
consisting of heavy metals at permissible limits in drinking water has been
shown to cause oxidative stress, genotoxicity and apoptosis in human aortic
smooth muscle cells. Considering that apoptosis in vascular smooth muscle cells
plays a role in the pathophysiology of diseases such as arteriosclerosis and
hypertension, we believe it would be beneficial to assess these limits through
extensive investigations in further studies.

Anahtar Kelimeler

Human aortic vascular smooth muscle cell, cardiovascular toxicity, heavy metal, gen expression

Genotoxic and Apoptotic Effects of Heavy Metal Mixture on Human Aortic Vascular Smooth Muscle Cell Line

Öz

Amaç: Ağır metaller özgül ağırlıkları
5g cm^-3’ten yüksek olan en yaygın kirleticilerdir. Insanların ağır
metallere maruziyeti genellikle ağır metal içeren su ve besinlerin tüketilmesi
yolu ile olmaktadır. Içme suyu yolu ile vücuda giren metaller genotoksisite ve
çeşitli hastalıklara neden olurlar. Bu nedenle insani tüketim amaçlı kullanılan
sularda ağır metallerin kabul edilebilir limitleri kanun ve yönetmelikler ile
belirlenmiştir. Bizim çalışmamız içme suyunda izin verilen limitlerde metal
miksinin (Cu, Zn, Pb ve Fe) insan aort düz kas hücrelerinde (TG-HA-VSMC)
etkilerini moleküler düzeyde araştırmayı amaçlamıştır.

Materyal-Metot: İnsan aort düz kas
hücrelerine içme sularında izin verilen düzey ve 10 kat yüksek konsantrasyonda
metal miksi 24 ve 48 saat süre ile uygulandı ve RAPD-DNA polimorfizmine ilaveten
antioksidan enzimler, ısı şok proteinleri ve apoptoz yolak genlerinin ifadeleri
belirlendi.

Bulgular: Ağır metal uygulamasının DNA
polimorfizminde, apoptotic hücre oranında, antioksidan ve apoptoz yolağı gen
ifadelerinde artışa neden olduğu belirlendi. 

Anahtar Kelimeler

İnsan aort vasküler düz kas hücresi, ağır metal, kardiyovasküler toksisite, gen ifadesi

Kaynakça

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