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Farklı Şiddetteki Periodontal Hastalıklarda Salya ve Serumdaki PTX-3 ve CRP Düzeylerinin Değerlendirilmesi

Year 2022, Volume: 13 Issue: 1, 19 - 29, 11.04.2022
https://doi.org/10.22312/sdusbed.1000228

Abstract

Amaç: Bu çalışmanın amacı periodontal açıdan sağlıklı ve farklı şiddetlerde periodontal hastalığı olan (gingivitis, periodontitis –Evre I ve Evre III) bireylerin salya ve serum örneklerinde Pentraksin (PTX)-3 ve C-reaktif protein (CRP) düzeylerinin belirlenmesidir.
Materyal-metot: Sağlıklı (Grup 1, n=20), gingivitis (Grup 2, n=20), Evre I periodontitisli (Grup 3) ve Evre III periodontitisli (Grup 4, n=20) olmak üzere 80 sigara içmeyen sistemik sağlıklı bireyin salya ve serum CRP ve PTX-3 düzeyleri ELISA yöntemiyle belirlenerek gruplar arasında karşılaştırıldı ve klinik parametrelerle korelasyonları incelendi.
Bulgular: En yüksek serum CRP düzeyi Grup 4’te belirlenirken, Grup 1 tüm gruplardan anlamlı düzeyde düşük serum CRP düzeyi sergiledi (p<0,05). Serum CRP düzeylerinin Grup 2 ve Grup 3’te istatistiksel olarak birbirine benzer (p>0,05); ancak Grup 1’den anlamlı yüksek (p<0,05) ve Grup 4’ten anlamlı düşük düzeyde (p<0,05)olduğu belirlendi. Serum PTX-3 sağlıklı gruptan şiddetli periodontitisli gruplarına doğru artan bir düzeyde sıralanmaktaydı; ancak bu sıralamada Grup 2 Grup 3’ten daha yüksek serum PTX-3 düzeyi sergiledi (p<0,05).
Salya CRP ve PTX-3 düzeyleri Grup 1’de diğer gruplardan anlamlı düşük, Grup 3 ve 4’te diğer gruplardan anlamlı yüksek düzeyde iken (p<0,05); Grup 2 ve Grup 3 birbirine benzer düzeyler sergiledi (p>0,05). Periodontal parametreler ve serum ve salya parametreleri arasındaki korelasyonlar incelendiğinde, hem CRP hem de PTX-3’ün tüm periodontal parametreler ile güçlü korelasyonlar sergilediği belirlendi (p<0,001).
Sonuç: İncelenen salya ve serum parametreleri (CRP ve PTX-3) ve çalışma grupları arasında belirlenen düzeysel farklılık ve parametreler arasındaki korelasyonlar periodontal hastalık-sistemik hastalık ilişkisini açıklamak için önemli veriler sunmuştur. 

Supporting Institution

Bu çalışma Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından 5108-DU1-17 proje numarası ile desteklenmiştir.

Project Number

5108-DU1-17

References

  • [1] Hegde, R., Awan, K.H. 2019. Effects of periodontal disease on systemic health. Disease A Month, 65(6),185-92.
  • [2] Otomo-Corgel, J., Pucher, J.J., Rethman, M.P., Reynolds, M.A. 2012. State of the science: chronic periodontitis and systemic health. Journal of Evidence Based Dental Practice, 12(3), 20-8.
  • [3] Schenkein, H.A., Loos, B.G. 2013. Inflammatory mechanisms linking periodontal diseases to cardiovascular diseases. Journal of Periodontology, 84(4 Suppl) S51–S69.
  • [4] Sanz, M., Marco Del Castillo, A., Jepsen, S., Gonzalez-Juanatey, J.R., D'Aiuto, F., Bouchard, P., et al. Periodontitis and cardiovascular diseases: Consensus report. 2020. Journal of Clinical Periodontology, 47(3), 268-88.
  • [5] Nehring, S.M., Goyal, A., Bansal, P., Patel, B.C. 2021. C Reactive Protein. StatPearls. 2021; PMID: 28722873
  • [6] PolePalle, T.S., Moogala, S., Pu, S.B., PeSala, S., Palagi, F.B. 2015. Acute phase proteins and their role in periodontitis: a review. Journal of Clinical Diagnostic Research, 9(11), ZE01-ZE05.
  • [7] Ebersole, J.L., Cappelli, D. 2000. Acute-phase reactants in infections and inflammatory diseases. Periodontology 2000, 23(1):19–49.
  • [8] Demmer, R.T., Trinquart, L., Zuk, A., Fu, B.C., Blomkvist, J., Michalowicz, B.S., et al. 2013. The influence of anti-infective periodontal treatment on C-reactive protein: a systematic review and meta-analysis of randomized controlled trials. PLoS One, 148(10), e77441.
  • [9] Garlanda, C., Bottazzi, B., Bastone, A., Mantovani, A. 2005. Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility. Annual Review of Immunology, 23, 337-66.
  • [10] Fujita, Y., Ito, H., Sekino, S., Numabe, Y. 2012. Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis. Odontology, 100(2), 215–21.
  • [11] Fazzini, F., Peri, G., Doni, A., Dell’Antonio, G., Dal Cin, E., Bozzolo, E, et al. 2001. PTX-3 in small-vessel vasculitides: An independent indicator of disease activity produced at sites of inflammation. Arthritis and Rheumatism, 44(12), 2841-50.
  • [12] Pradeep, A., Kathariya, R., Raghavendra, N., Sharma, A. 2011. Levels of pentraxin‐ 3 in gingival crevicular fluid and plasma in periodontal health and disease. Journal of Periodontology, 82(5), 734-41.
  • [13] Gümüş, P., Nizam, N., Nalbantsoy, A., Özçaka, Ö., Buduneli, N. 2014. Saliva and serum levels of pentraxin‐ 3 and interleukin‐ 1β in generalized aggressive or chronic periodontitis. Journal of Periodontology, 85(3), e40-e6.
  • [14] Çalapkorur, M.U., Alkan, B.A., Tasdemir, Z., Akcali, Y., Saatçi, E. 2017. Association of peripheral arterial disease with periodontal disease: analysis of inflammatory cytokines and an acute phase pro-tein in gingival crevicular fluid and serum. Journal of Periodontal Research, 52(3), 532-9.
  • [15] Folwaczny, M., Karnesi, E., Berger, T., Paschos, E. 2017. Clinical association between chronic periodontitis and the leukocyte extravasation inhibitors developmental endothelial locus-1 and pentraxin-3. European Journal of Oral Science, 125(4), 258–64.
  • [16] Papapanou, P.N., Sanz, M., Buduneli, N., Dietrich, T., Feres, M., Fine, D.H., et al. 2018. Periodontitis: consensus report of workgroup 2 of the 2017 World Workshop on the classification of periodontal and peri-implant diseases and conditions. Journal of Periodontology, 89 (Suppl 1), S173-82. [17] Chapple, I.L.C., Mealey, B., Van Dyke, T.E., Bartold, P.M., Dommisch, H., Eickholz, P., et al. 2018. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. Journal of Periodontology, 89 (Suppl 1), S74-S84.
  • [18] Armitage, G.C. 2004. Periodontal diagnoses and classification of periodontal diseases. Periodontology 2000, 34(1), 9-21. [19] Löe, H. 1967. The gingival index, the plaque index and the retention index systems. Journal of Periodontology, 38(6 Suppl), 610-6.
  • [20] Löe, H., Silness, J. 1963. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontologica Scandnavica, 21(6), 533-51.
  • [21] Lobene, R.R., Weatherford, T., Ross, N.M., Lamm, R.A., Menaker, L. 1986. A modified gingival index for use in clinical trials. Clinical Preventive Dentistry, 8(1), 3-6.
  • [22] Nesse, W., Abbas, F., van der Ploeg, I., Spijkervet, F.K., Dijkstra, P.U., Vissink, A. 2008. Periodontal inflamed surface area: quantifying inflammatory burden. Journal of Clinical Periodontology, 35(8), 668-73.
  • [23] Hujoel, P., White, B., Garcia, R., Listgarten, M. 2001. The dentogingival epithelial surface area revisited. Journal of Periodontal Research, 36(1), 48-55.
  • [24] Leira, Y., Martín-Lancharro, P., Blanco, J. 2018. Periodontal inflamed surface area and periodontal case definition classification. Acta Odontologica Scandnavica, 2018,76(3), 195-8.
  • [25] Navazesh, M., Kumar, S.K.S. 2008. Measuring salivary flow challenges and opportunities. Journal of American Dental Association, 139 (Suppl), 35S-40S.
  • [26] Keles, G.C., Cetinkaya, B.O., Simsek, S.B., Koprulu, D., Kahraman, H. 2007. The role of periodontal disease on acute phase proteins in patients with coronary heart disease and diabetes. Turkish Journal of Medical Sciences, 37(1), 39-44.
  • [27] Loos, B.G. 2005. Systemic markers of inflammation in periodontitis. Journal of Periodontology, 76(11 Suppl), 2106-15.
  • [28] Fitzsimmons, T.R., Sanders, A.E., Bartold, P.M., Slade, G.D. 2010. Local and systemic biomarkers in gingival crevicular fluid increase odds of periodontitis. Journal of Clinical Periodontology, 37(1), 30-6. [29] Megson, E., Fitzsimmons, T., Dharmapatni, K., Bartold, P.M. 2010. C-reactive protein in gingival crevicular fluid may be indicative of systemic inflammation. Journal of Clinical Periodontology, 37(9), 797–804.
  • [30] Lakshmanan, R., Jayakumar, N., Sankari, M., Padmalatha, O., Varghese, S. 2014. Estimation of Pentraxin‐ 3 levels in the gingival tissues of chronic and aggressive periodontitis participants: an in vivo study. Journal of Periodontology, 85(2), 290-7.
  • [31] Varghese, M., Varghese, S., Jayakumar, D.M. 2015. Evaluation of pentraxins 3 in chronic periodontitis patients before and after the treatment. International Journal of Medical and Exercise Science, 1(1), 9-15.
  • [32] Koenig, W. 2013. High-sensitivity C-reactive protein and atherosclerotic disease: from improved risk prediction to risk-guided therapy. International Journal of Cardiology, 168(6), 5126-34.
  • [33] Nerkiz, P., Doganer, Y.C., Aydogan, U., Akbulut, H., Parlak, A., Aydogdu, A., et al. 2015. Serum pentraxin-3 level in patients who underwent coronary angiography and relationship with coronary atherosclerosis. Medical Principals and Practice, 24(4), 369-75.
  • [34] Temelli, B., Yetkin Ay, Z., Savaş, H.B., Aksoy, F., Kumbul Doğuç, D., Uskun, E., et al. 2018. Circulation levels of acute phase proteins pentraxin 3 and serum amyloid A in atherosclerosis have correlations with periodontal inflamed surface area. Journal of Applied Oral Science, 26, e20170322.
  • [35] Arboleda, S., Vargas, M., Losada,, S, Pinto, A. 2019. Review of obesity and periodontitis: an epidemiological view. British Dental Journal, 227(3), 235-9.
  • [36] Buduneli, N., Özçaka, Ö., Nalbantsoy, A. 2011. Salivary and plasma levels of toll-like receptor 2 and toll-like receptor 4 in chronic periodontitis. Journal of Periodontology, 82(6), 878-84.
  • [37] Tan, A., Gürbüz, N., Özbalci, F.İ., Koşkan, Ö., Yetkin Ay, Z. 2020. Increase in serum and salivary neutrophil gelatinase-associated lipocalin levels with increased periodontal inflammation. Journal of Applied Oral Science, 28, e20200276.

The Evaluation of Salivary and Serum PTX-3 and CRP Levels in Periodontal Diseases of Different Severity

Year 2022, Volume: 13 Issue: 1, 19 - 29, 11.04.2022
https://doi.org/10.22312/sdusbed.1000228

Abstract

Objective: The aim of this study is to evaluate the salivary and serum levels of Pentraxin (PTX)-3 and C-reactive protein (CRP) of individuals who are periodontally healthy or have periodontal diseases with different severity (gingivitis, periodontitis – Stage I and Stage III).
Material-Method: Salivary and serum CRP and PTX-3 levels of 80 non-smoker systemically healthy individuals (periodontally healthy (Group 1), gingivitis (Group 2), Stage I periodontitis (Group 3) and Stage III periodontitis (Group 4) were determined by ELISA, compared among the groups and their correlations were analyzed.
Results: While the highest serum CRP level was determined in Group 4, Group 1 showed significantly lower serum CRP levels from all groups (p<0.05). Group 2 and 3 had similar serum CRP levels (p>0.05), but statistically significantly higher serum CRP levels were found in both groups than Group 1 and lower than Group 4 (p<0.05). Serum PTX-3 was ranked increasing from the healthy group to the groups with severe periodontitis; however, Group 2 presented significantly higher serum PTX-3 level than Group 3 (p<0.05).While salivary CRP and PTX-3 levels were significantly lower in Group 1 than the other groups, and significantly higher in Groups 3 and 4 than the other groups (p<0.05); Group 2 and Group 3 presented similar levels (p>0.05). When the correlations between periodontal parameters and serum and saliva parameters were examined, both CRP and PTX-3 showed strong correlations with all periodontal parameters (p<0.001).
Conclusion: The significant differences between the salivary and serum parameters’ levels among the study groups and the correlations between the parameters provided important data to clarify the periodontal disease-systemic disease relationship.

Project Number

5108-DU1-17

References

  • [1] Hegde, R., Awan, K.H. 2019. Effects of periodontal disease on systemic health. Disease A Month, 65(6),185-92.
  • [2] Otomo-Corgel, J., Pucher, J.J., Rethman, M.P., Reynolds, M.A. 2012. State of the science: chronic periodontitis and systemic health. Journal of Evidence Based Dental Practice, 12(3), 20-8.
  • [3] Schenkein, H.A., Loos, B.G. 2013. Inflammatory mechanisms linking periodontal diseases to cardiovascular diseases. Journal of Periodontology, 84(4 Suppl) S51–S69.
  • [4] Sanz, M., Marco Del Castillo, A., Jepsen, S., Gonzalez-Juanatey, J.R., D'Aiuto, F., Bouchard, P., et al. Periodontitis and cardiovascular diseases: Consensus report. 2020. Journal of Clinical Periodontology, 47(3), 268-88.
  • [5] Nehring, S.M., Goyal, A., Bansal, P., Patel, B.C. 2021. C Reactive Protein. StatPearls. 2021; PMID: 28722873
  • [6] PolePalle, T.S., Moogala, S., Pu, S.B., PeSala, S., Palagi, F.B. 2015. Acute phase proteins and their role in periodontitis: a review. Journal of Clinical Diagnostic Research, 9(11), ZE01-ZE05.
  • [7] Ebersole, J.L., Cappelli, D. 2000. Acute-phase reactants in infections and inflammatory diseases. Periodontology 2000, 23(1):19–49.
  • [8] Demmer, R.T., Trinquart, L., Zuk, A., Fu, B.C., Blomkvist, J., Michalowicz, B.S., et al. 2013. The influence of anti-infective periodontal treatment on C-reactive protein: a systematic review and meta-analysis of randomized controlled trials. PLoS One, 148(10), e77441.
  • [9] Garlanda, C., Bottazzi, B., Bastone, A., Mantovani, A. 2005. Pentraxins at the crossroads between innate immunity, inflammation, matrix deposition, and female fertility. Annual Review of Immunology, 23, 337-66.
  • [10] Fujita, Y., Ito, H., Sekino, S., Numabe, Y. 2012. Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis. Odontology, 100(2), 215–21.
  • [11] Fazzini, F., Peri, G., Doni, A., Dell’Antonio, G., Dal Cin, E., Bozzolo, E, et al. 2001. PTX-3 in small-vessel vasculitides: An independent indicator of disease activity produced at sites of inflammation. Arthritis and Rheumatism, 44(12), 2841-50.
  • [12] Pradeep, A., Kathariya, R., Raghavendra, N., Sharma, A. 2011. Levels of pentraxin‐ 3 in gingival crevicular fluid and plasma in periodontal health and disease. Journal of Periodontology, 82(5), 734-41.
  • [13] Gümüş, P., Nizam, N., Nalbantsoy, A., Özçaka, Ö., Buduneli, N. 2014. Saliva and serum levels of pentraxin‐ 3 and interleukin‐ 1β in generalized aggressive or chronic periodontitis. Journal of Periodontology, 85(3), e40-e6.
  • [14] Çalapkorur, M.U., Alkan, B.A., Tasdemir, Z., Akcali, Y., Saatçi, E. 2017. Association of peripheral arterial disease with periodontal disease: analysis of inflammatory cytokines and an acute phase pro-tein in gingival crevicular fluid and serum. Journal of Periodontal Research, 52(3), 532-9.
  • [15] Folwaczny, M., Karnesi, E., Berger, T., Paschos, E. 2017. Clinical association between chronic periodontitis and the leukocyte extravasation inhibitors developmental endothelial locus-1 and pentraxin-3. European Journal of Oral Science, 125(4), 258–64.
  • [16] Papapanou, P.N., Sanz, M., Buduneli, N., Dietrich, T., Feres, M., Fine, D.H., et al. 2018. Periodontitis: consensus report of workgroup 2 of the 2017 World Workshop on the classification of periodontal and peri-implant diseases and conditions. Journal of Periodontology, 89 (Suppl 1), S173-82. [17] Chapple, I.L.C., Mealey, B., Van Dyke, T.E., Bartold, P.M., Dommisch, H., Eickholz, P., et al. 2018. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. Journal of Periodontology, 89 (Suppl 1), S74-S84.
  • [18] Armitage, G.C. 2004. Periodontal diagnoses and classification of periodontal diseases. Periodontology 2000, 34(1), 9-21. [19] Löe, H. 1967. The gingival index, the plaque index and the retention index systems. Journal of Periodontology, 38(6 Suppl), 610-6.
  • [20] Löe, H., Silness, J. 1963. Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontologica Scandnavica, 21(6), 533-51.
  • [21] Lobene, R.R., Weatherford, T., Ross, N.M., Lamm, R.A., Menaker, L. 1986. A modified gingival index for use in clinical trials. Clinical Preventive Dentistry, 8(1), 3-6.
  • [22] Nesse, W., Abbas, F., van der Ploeg, I., Spijkervet, F.K., Dijkstra, P.U., Vissink, A. 2008. Periodontal inflamed surface area: quantifying inflammatory burden. Journal of Clinical Periodontology, 35(8), 668-73.
  • [23] Hujoel, P., White, B., Garcia, R., Listgarten, M. 2001. The dentogingival epithelial surface area revisited. Journal of Periodontal Research, 36(1), 48-55.
  • [24] Leira, Y., Martín-Lancharro, P., Blanco, J. 2018. Periodontal inflamed surface area and periodontal case definition classification. Acta Odontologica Scandnavica, 2018,76(3), 195-8.
  • [25] Navazesh, M., Kumar, S.K.S. 2008. Measuring salivary flow challenges and opportunities. Journal of American Dental Association, 139 (Suppl), 35S-40S.
  • [26] Keles, G.C., Cetinkaya, B.O., Simsek, S.B., Koprulu, D., Kahraman, H. 2007. The role of periodontal disease on acute phase proteins in patients with coronary heart disease and diabetes. Turkish Journal of Medical Sciences, 37(1), 39-44.
  • [27] Loos, B.G. 2005. Systemic markers of inflammation in periodontitis. Journal of Periodontology, 76(11 Suppl), 2106-15.
  • [28] Fitzsimmons, T.R., Sanders, A.E., Bartold, P.M., Slade, G.D. 2010. Local and systemic biomarkers in gingival crevicular fluid increase odds of periodontitis. Journal of Clinical Periodontology, 37(1), 30-6. [29] Megson, E., Fitzsimmons, T., Dharmapatni, K., Bartold, P.M. 2010. C-reactive protein in gingival crevicular fluid may be indicative of systemic inflammation. Journal of Clinical Periodontology, 37(9), 797–804.
  • [30] Lakshmanan, R., Jayakumar, N., Sankari, M., Padmalatha, O., Varghese, S. 2014. Estimation of Pentraxin‐ 3 levels in the gingival tissues of chronic and aggressive periodontitis participants: an in vivo study. Journal of Periodontology, 85(2), 290-7.
  • [31] Varghese, M., Varghese, S., Jayakumar, D.M. 2015. Evaluation of pentraxins 3 in chronic periodontitis patients before and after the treatment. International Journal of Medical and Exercise Science, 1(1), 9-15.
  • [32] Koenig, W. 2013. High-sensitivity C-reactive protein and atherosclerotic disease: from improved risk prediction to risk-guided therapy. International Journal of Cardiology, 168(6), 5126-34.
  • [33] Nerkiz, P., Doganer, Y.C., Aydogan, U., Akbulut, H., Parlak, A., Aydogdu, A., et al. 2015. Serum pentraxin-3 level in patients who underwent coronary angiography and relationship with coronary atherosclerosis. Medical Principals and Practice, 24(4), 369-75.
  • [34] Temelli, B., Yetkin Ay, Z., Savaş, H.B., Aksoy, F., Kumbul Doğuç, D., Uskun, E., et al. 2018. Circulation levels of acute phase proteins pentraxin 3 and serum amyloid A in atherosclerosis have correlations with periodontal inflamed surface area. Journal of Applied Oral Science, 26, e20170322.
  • [35] Arboleda, S., Vargas, M., Losada,, S, Pinto, A. 2019. Review of obesity and periodontitis: an epidemiological view. British Dental Journal, 227(3), 235-9.
  • [36] Buduneli, N., Özçaka, Ö., Nalbantsoy, A. 2011. Salivary and plasma levels of toll-like receptor 2 and toll-like receptor 4 in chronic periodontitis. Journal of Periodontology, 82(6), 878-84.
  • [37] Tan, A., Gürbüz, N., Özbalci, F.İ., Koşkan, Ö., Yetkin Ay, Z. 2020. Increase in serum and salivary neutrophil gelatinase-associated lipocalin levels with increased periodontal inflammation. Journal of Applied Oral Science, 28, e20200276.
There are 34 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Araştırma Articlesi
Authors

Aykut Tan This is me 0000-0001-5241-4510

Nilgün Gürbüz 0000-0003-4476-5593

Furkan İlker Özbalcı 0000-0002-0086-4090

Özgür Koşkan 0000-0002-5089-6250

Prof. Dr. Zuhal Yetkin Ay 0000-0003-4716-9404

Project Number 5108-DU1-17
Publication Date April 11, 2022
Submission Date September 24, 2021
Published in Issue Year 2022 Volume: 13 Issue: 1

Cite

Vancouver Tan A, Gürbüz N, Özbalcı Fİ, Koşkan Ö, Yetkin Ay PDZ. Farklı Şiddetteki Periodontal Hastalıklarda Salya ve Serumdaki PTX-3 ve CRP Düzeylerinin Değerlendirilmesi. Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi. 2022;13(1):19-2.

SDÜ Sağlık Bilimleri Dergisi, makalenin gönderilmesi ve yayınlanması dahil olmak üzere hiçbir aşamada herhangi bir ücret talep etmemektedir. Dergimiz, bilimsel araştırmaları okuyucuya ücretsiz sunmanın bilginin küresel paylaşımını artıracağı ilkesini benimseyerek, içeriğine anında açık erişim sağlamaktadır.