Evaluation of the Effect of Pinus nigra L. and Pinus brutia L. (Pine) Alcoholic Bark Extracts on Production Potential of Virulence Factors About Anti-QS Activity Against Pseudomonas aeruginosa and Chromobacterium violaceum

Year 2022, Volume: 13 Issue: 2, 171 - 178, 31.08.2022

Bashar Ibrahım

https://doi.org/10.22312/sdusbed.1129144

Abstract

The apparent Quorum Sensing (QS) inhibition activity of plants could be used as a new intervention strategy to reduce bacterial pathogenicity. This study, it was aimed to investigate the activities of the bark extracts of two different pine species (Pinus nigra L. and Pinus brutia L) prepared with methanol on the production potential of Quorum Sensing (QS)-dependent virulence factors of Pseudomonas aeruginosa PAO1 and Chromobacterium violaceum bacteria. Ultrasonic-assisted extractions were performed to obtain the extracts. The results showed that the extracts were statistically significant at different rates on both bacterial strains, but the P. nigra bark extract was more effective. Pinus brutia bark extract showed an inhibition rate (77%) on swarming motility. The bark extract of Pinus nigra had a higher inhibition rate (80%). In addition, the inhibition effect of Pinus nigra bark extract against P. aeruginosa PAO1 on pyocyanin production and biofilm formation other than elastase, inhibition rates of 62% and 54%, respectively, were higher than Pinus brutia bark extract (41%, 51%). Our study revealed that Pinus nigra L. and Pinus brutia L. bark extracts strongly interfere with the phenotype, including pyocyanin, elastase, Violasein production, biofilm formation, and swarm motility. These results may indicate the potential use of pine bark extracts as anti-QS agents.

Keywords

Pinus nigra L., Pinus brutia L., Pseudomonas aeruginosa PAO1, Chromobacterium violaceum, Quorum Sensing, Virulence

Pinus nigra L. ve Pinus brutia L. (Çam) Alkollü Kabuk Ekstraktlarının Pseudomonas aeruginosa ve Chromobacterium violaceum'a Karşı Anti-QS Aktivitesi Yoluyla Virülans Faktörlerinin Üretim Potansiyeli Üzerindeki Etkisinin Değerlendirilmesi

Abstract

Bitkilerin belirgin çevreyi algılama (QS) inhibisyon aktivitesi, bakteriyel patojeniteyi azaltmak için yeni bir müdahale stratejisi olarak kullanılabilir. Bu çalışmada, metanol ile hazırlanan iki farklı çam türü (Pinus nigra L.ve Pinus brutia L.) kabuk ekstraktlarının, Pseudomonas aeruginosa PAO1 ve Chromobacterium violaceum bakterilerin Quorum Sensing (QS)'ye bağlı virülans faktörlerinin üretim potansiyeli üzerindeki aktivitelerinin araştırılması amaçlanmıştır. Ekstraktları, elde etmek için ultrasonik destekli ekstraksiyonlar yapılmıştır. Sonuçlar, ekstraktların her iki bakteri suşu üzerinde farklı oranlarla istatistiksel yönden anlamlı olmakla birlikte P. nigra kabuk ekstraktın daha etkili olduğunu göstermiştir. Pinus brutia L ekstraktının, kayma hareketi üzerinde inhibisyon oranı (%77) gösterirken Pinus nigra L ekstraktının inhibisyon oranı (%80) daha yüksek bulunmuştur.. Ayrıca, Pinus nigra ekstraktının P. aeruginosa PAO1’e karşı elastaz dışında piyosiyanin üretimi ve biyofilm oluşumu üzerindeki inhibisyon etkisi, sırasıyla %62 ve %54 olarak Pinus brutia ekstraktına göre (%41,%51) daha yüksek düzeyde tespit edilmiştir. Çalışma sonucunda elde edilen veriler P. nigra ve P. brutia ekstraktlarının piyosiyanin, elastaz, viyolasein üretimi, biyofilm oluşumu ve kayma hareketliği dahil olmak üzere fenotipe güçlü bir şekilde müdahale ettiğini ortaya çıkarmıştır. Bu sonuçlar, çam kabuğu ekstraktlarının anti-QS ajanlar olarak potansiyele sahip olabileceğini göstermiştir.

Keywords

Pinus nigra L, Pinus brutia L., Pseudomonas aeruginosa PAO1, Chromobacterium violaceum, Çevreyi algılama, virülans

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