AKUT İSKEMİK İNMELİ HASTALARDA IL-18 VE ADROPİN DÜZEYLERİ

Year 2022, , 187 - 195, 30.06.2022

Alper Eren , İbrahim İyigün

https://doi.org/10.17343/sdutfd.1002269

Abstract

Amaç
İnmeli hastalarda disfonksiyonel vasküler olaylara yol
açan önde gelen faktörlerden bir tanesi olan ateroskleroz;
endotelyal disfonksiyon ve vasküler inflamasyonun
önemli bir rol oynadığı çok faktörlü ve kompleks
bir süreçtir. Biz bu çalışmada endotel disfonksiyonu
ve inflamatuar süreçlerle ilişkisi gösterilmiş olan IL-
18 ve adropininin akut iskemik inme hastalarındaki
serum düzeyleri ile epidemiyolojik, klinik, radyolojik
bulgular ve inme şiddeti arasındaki ilişkiyi araştırmayı
amaçladık.
Gereç ve Yöntem
Çalışmamıza akut iskemik inme tanısı konulan 61
hasta ve kontrol grubu olarak 30 sağlıklı birey alındı.
Hasta grubunda etiyolojik ve klinik olarak inme alt
grupları ve inme şiddeti belirlendi. Hasta grubundan
ilk 24 saatte, kontrol grubundan herhangi bir zamanda
venöz kan örnekleri alınarak serumları ayrıldı ve
-80⁰C’de saklandı. ELISA yöntemi kullanılarak IL-8 ve
adropin düzeyleri belirlendi. Hasta ve kontrol gruplarının
IL-18 ve adropin düzeyleri ile iskemik inme arasındaki
ilişkiler istatistiksel olarak analiz edildi.
Bulgular
Adropin düzeyi hasta grubunda kontrol grubuna göre
istatistiksel olarak anlamlı derecede düşüktü (sırasıyla
398.01±403.51 ve 509.42±1492.89; p=0.041). Çalışma
ve kontrol gruplarının IL-18 düzeyleri benzerdi
(sırasıyla 24.87±14.26 ve 21.11±14.93; p=0.112).
İnme risk faktörleri, inme alt grupları ve inme şiddeti
ile belirlenen IL-18 ve adropin düzeyleri arasında ilişki
yoktu.
Sonuç
Bu bulgular, düşük adropin düzeylerinin ateroskleroz
göstergesi olarak iskemik inme risk tahmini ölçeklerinde
kullanılabileceğini göstermiştir. Akut iskemik inmeli
hasta grubu ile kontrol grubu arasında ortalama
serum IL-18 düzeyi açısından fark olmaması, IL-18'in
iskemiye bağlı inflamasyonda geç dönem bir sitokin
olarak rol oynayabileceğini düşündürmüştür.

Keywords

iskemik inme, adropin, IL-18, inflamasyon, endotel disfonksiyonu

Supporting Institution

Yok

Project Number

Tez no: 462412

Thanks

Atatürk Üniversitesi Tıp Fakültesi Tıbbi Biyokimya AD öğretim üyesi Prof. Dr. Ahmet KIZILTUNÇ hocama makalemin fikir ve labaoratuar aşamasındaki her türlü desteği için çok teşekkür ediyorum.

IL-18 AND ADROPIN LEVELS IN PATIENTS WITH ACUTE ISCHEMIC STROKE

Abstract

Objective
Atherosclerosis, one of the prominent factors causing
dysfunctional vascular events in stroke patients,
is a multi-factorial and complex process in which
endothelial dysfunction and vascular inflammation
play significant roles. This study aimed to investigate
the relationships between serum levels of IL-18 and
adropin, associated with endothelial dysfunction and
inflammatory processes in acute ischemic stroke
patients, with epidemiological, clinical, radiological
findings and stroke severity.
Materials and Methods
Sixty-one patients diagnosed with acute ischemic
stroke and 30 healthy individuals were included in the
study as the patient and control groups. In the patient
group, the stroke sub-groups and severity were
determined etiologically and clinically. Venous blood
samples were obtained within the first 24 hours in the
patient group, and at any time in the control group,
their serums were separated and stored at -80°C. IL-8
and adropin levels were determined using the ELISA
method. The relationships between patient and
control groups’ IL-18 and adropin levels and ischemic
stroke were analyzed statistically.
Results
The adropin level was statistically significantly
lower in the patient group than the control group
(398.01±403.51 and 509.42±1492.89, respectively;
p=0.041). The IL-18 levels of the study and control
groups were similar (24.87±14.26 and 21.11±14.93,
respectively; p=0.112). There was no relationship
between the IL-18 and adropin levels determined
with stroke risk factors, stroke sub-groups, and stroke
severity.
Conclusion
These results showed that low adropin levels could be
used to indicate atherosclerosis in the risk prediction
scales of ischemic stroke. The absence of a difference
between the patient group with acute ischemic stroke
and the control group regarding the first 24-hour mean
serum IL-18 level suggested that IL-18 could play
a role as a late-stage cytokine in ischemia-related
inflammation.

Keywords

ischemic stroke, adropin, IL-18, inflammation, endothelial dysfunction

Project Number

Tez no: 462412

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