COMPARING THE EFFECTS OF SILDENAFIL CITRATE AND DEXPANTHENOL ON WOUND HEALING: AN EXPERIMENTAL STUDY

Year 2022, , 368 - 377, 30.09.2022

Musluh Hakseven , Murat Kapan , Ulas Alabalık , Gökhan Avşar

https://doi.org/10.17343/sdutfd.1100763

Cited By: 1

Abstract

Objective
Wound healing is a serious problem that causes
high morbidity. Different pharmacological agents
have been studied to accelerate wound healing and
to prevent necrosis and ischemia, and still continue
to work. Topical dexpanthenol is widely used in both
ağırlığınwound
healing and various dermatological diseases.
The aim of this study was to compare the effects of
topical sildenafil citrate and topical dexpanthenol on
wound healing in rats.
Material and Method
A total of 30 female Wistar Albino rats, weighing 200-
250 gr, were used in the study. Rats were divided
into 3 groups. A linear 2 cm full-thickness incision
was made in the rat’s skin of the back. Steril saline
irrigation was applied to all animals in the control group.
Dexpanthenol was administered locally to the wounds
of the rats in the second group, and 10% sildenafil
citrate in the third group. Wound dimensions were
measured on the 3rd, 5th, 7th, 10th, 14th, and 21st
days. The full-thickness skin, including the incision,
were removed for histopathological examination on
the 21st days.
Results
We believe that sildenafil citrate can be used as
a topical cream for wound healing, as it leads to
significant reduction in wound size.
Conclusion
Although there was no statistically significant
difference, we observed that the wound healing was
better in the group where sildenafil citrate was applied
locally on the wound.

Keywords

Wound healing, sildenafil sitrate, dexpanthenol, rats

Project Number

Yok

SİLDENAFİL SİTRAT VE DEKSPANTENOLÜN YARA İYİLEŞMESİ ÜZERİNDEKİ ETKİLERİNİN KARŞILAŞTIRILMASI: DENEYSEL ÇALIŞMA

Abstract

Amaç
Yara iyileşmesi cerrahide yüksek morbiditeye neden
olabilen ciddi bir problemdir. Yara iyileşmesini hızlandırmak,
nekrozu veya iskemiyi önlemek için değişik
farmakolojik ajanlar çalışılmış ve halen yaygın olarak
çalışılmaya devam etmektedir. Yara iyileşmesinde
klinik pratikte topikal dekspantenolün geniş kulanım
alanları mevcuttur. Topikal dekspantenol hem yara
bakımında hem de dermatolojik hastalıkların tedavisinde
kullanılmaktadır. Bu çalışmamızda, ratlarda
oluşturulan deneysel yara modelinde lokal sildenafil
sitrat ve topikal dekspantenol uygulamalarının etkilerinin
karşılaştırılması amaçlanmıştır.
Gereç ve Yöntem
Çalışmada 200–250 gram arasında değişen 30 Adet
Wistar Albino dişi rat kullanıldı. Hayvanlar üç gruba
ayrıldı. Deney hayvanlarının anestezisini takiben sırt
orta kesimlerine 2 cm uzunluğunda tam kat kalınlıkta
deri defekti oluşturuldu. Yara pansumanında tüm hayvanların
yaraları steril % 0.9 NaCl solüsyonu ile yıkandıktan
sonra kontrol grubu olduğu gibi bırakılırken,
irigasyon sonrası 2. gruba %5’lik dekspantenol krem,
3. gruba % 10’luk sildenafil krem uygulandı. Ratlarda
yara boyutları 3., 5., 7., 10., 14. ve 21. günlerde ölçülerek
kaydedildi ve 21. gün tüm ratların sırt kısımlarından
insizyon hattını içerecek şekilde tam kat deri
çıkarılarak histolojik incelemeye alındı.
Bulgular
Tüm gruplara genel olarak bakıldığında günlere göre
yara boyutlarındaki küçülme ve histopatolojik bulgular
açısından istatiksel olarak anlamlı fark saptanmadı.
Sonuç
Sildenafil sitratın yara boyutunda önemli bir azalmaya
yol açtığı için yara iyileşmesi için topikal bir krem olarak
kullanılabileceğine inanıyoruz.

Keywords

Yara iyileşmesi, sildenafil sitrat, dekspantenol, rat

Supporting Institution

Destekleyen kurum yok

Project Number

Yok

References

• 1. Proksch, E., de Bony, R., Trapp, S., & Boudon, S. (2017). Topical use of dexpanthenol: a 70th anniversary article. The Journal of dermatological treatment 28(8), 766–773
• 2. Jamshidzadeh A, Azarpira N. (2011). The effects of topical sildenafil on wound healing in rat. Iranian Journal of Pharmaceutical Sciences 7(1): 43-48
• 3. Jarrahi, M., & Vafaei, A. A. (2004). Effect of topical phenytoin cream on linear incisional wound healing in albino rats. Daru, 12(4), 156-158.
• 4. Velnar, T., Bailey, T., & Smrkolj, V. (2009). The wound healing process: an overview of the cellular and molecular mechanisms. The Journal of international medical research, 37(5), 1528–1542.
• 5. Erdem, C., & Çelebi, C. R. (Eds.). (1996). Tüm yönleriyle yara iyileşmesi. Türk Dermatopatoloji Derneği.
• 6. Derici, H., Kamer, E., Ünalp, H. R., Diniz, G., Bozdag, A. D., Tansug, T., ... & Erbil, Y. (2010). Effect of sildenafil on wound healing: an experimental study. Langenbeck's archives of surgery, 395(6), 713-718.
• 7. Soneja, A., Drews, M., & Malinski, T. (2005). Role of nitric oxide, nitroxidative and oxidative stress in wound healing. Pharmacological reports, 57, 108.
• 8. Martin, P. (1997). Wound healing--aiming for perfect skin regeneration. Science, 276(5309), 75-81.
• 9. Ziche, M., & Morbidelli, L. (2000). Nitric oxide and angiogenesis. Journal of neuro-oncology, 50(1), 139-148.
• 10. Ebner, F., Heller, A., Rippke, F., & Tausch, I. (2002). Topical use of dexpanthenol in skin disorders. American journal of clinical dermatology, 3(6), 427-433.
• 11. Romiti, R., & Romiti, N. (2002). Dexpanthenol cream significantly improves mucocutaneous side effects associated with isotretinoin therapy. Pediatric dermatology, 19(4), 368-371.
• 12. Schmuth, M., Wimmer, M. A., Hofer, S., Sztankay, A., Weinlich, G., Linder, D. M., ... & Fritsch, E. (2002). Topical corticosteroid therapy for acute radiation dermatitis: a prospective, randomized, double‐blind study. British Journal of Dermatology, 146(6), 983-991.
• 13. Lee, R. H., Efron, D., Tantry, U., & Barbul, A. (2001). Nitric oxide in the healing wound: a time-course study. Journal of Surgical Research, 101(1), 104-108.
• 14. Chung, A. W., Radomski, A., Alonso-Escolano, D., Jurasz, P., Stewart, M. W., Malinski, T., et al. (2004). Platelet–leukocyte aggregation induced by PAR agonists: regulation by nitric oxide and matrix metalloproteinases. British journal of pharmacology, 143(7), 845.
• 15. Cooke, J. P., & Losordo, D. W. (2002). Nitric oxide and angiogenesis. Circulation, 105(18), 2133–2135.
• 16. Schwentker, A., Vodovotz, Y., Weller, R., & Billiar, T. R. (2002). Nitric oxide and wound repair: role of cytokines?. Nitric oxide, 7(1), 1-10.
• 17. Stallmeyer, B., Kämpfer, H., Kolb, N., Pfeilschifter, J., & Frank, S. (1999). The function of nitric oxide in wound repair: inhibition of inducible nitric oxide-synthase severely impairs wound reepithelialization. Journal of Investigative Dermatology, 113(6), 1090-1098.
• 18. Nakamura, H., Herzenberg, L. A., Bai, J., Araya, S., Kondo, N., Nishinaka, Y., et al. (2001). Circulating thioredoxin suppresses lipopolysaccharide-induced neutrophil chemotaxis. Proceedings of the National Academy of Sciences, 98(26), 15143-15148.
• 19. Furchgott, R. F. (1996). The discovery of endothelium-derived relaxing factor and its importance in the identification of nitric oxide. Jama, 276(14), 1186-1188.
• 20. Weller, R., Schwentker, A., Billiar, T. R., & Vodovotz, Y. (2003). Autologous nitric oxide protects mouse and human keratinocytes from ultraviolet B radiation-induced apoptosis. American Journal of Physiology-Cell Physiology, 284(5), C1140-C1148.
• 21. Krischel, V., Bruch-Gerharz, D., Suschek, C., Kröncke, K. D., Ruzicka, T., & Kolb-Bachofen, V. (1998). Biphasic effect of exogenous nitric oxide on proliferation and differentiation in skin derived keratinocytes but not fibroblasts. Journal of Investigative Dermatology, 111(2), 286-291.
• 22. Stamler, J. S., Jia, L., Eu, J. P., McMahon, T. J., Demchenko, I. T., Bonaventura, J., et al. (1997). Blood flow regulation by S-nitrosohemoglobin in the physiological oxygen gradient. Science, 276(5321), 2034-2037.
• 23. Weller, R., Ormerod, A. D., Hobson, R. P., & Benjamin, N. J. (1998). A randomized trial of acidified nitrite cream in the treatment of tinea pedis. Journal of the American Academy of Dermatology, 38(4), 559-563.
• 24. Salcido, R. (2008). Viagra and wound healing: the NO connection. Advances in skin & wound care, 21(3), 106-109.
• 25. Sarifakioglu, N., Gokrem, S., Ates, L., Akbuga, U. B., & Aslan, G. (2004). The influence of sildenafil on random skin flap survival in rats: an experimental study. British journal of plastic surgery, 57(8), 769-772.
• 26. Farsaie, S., Khalili, H., Karimzadeh, I., & Dashti-Khavidaki, S. (2012). An old drug for a new application: potential benefits of sildenafil in wound healing. Journal of Pharmacy & Pharmaceutical Sciences, 15(4), 483-498.
• 27. Ayyıldız, A., Nuhoglu, B., Huri, E., Uysal, A., Üstün, H., Germiyanoglu, C., et al. (2005). Experimental study: The efficacy and dosage of sildenafil citrate on flap viability. Turk J Urol, 31, 163-169.