RATLARDA AMİODARONA BAĞLI AKCİĞER TOKSİSİTESİ ÜZERİNE GİLABURU (Viburnum opulus L.)’NUN ETKİSİ

Year 2023, , 324 - 332, 23.09.2023

Dilek Bayram , Nazife Karakeçi

https://doi.org/10.17343/sdutfd.1199352

Abstract

Amaç
Amiodaron (AD), benzofuran türevi, yapısal olarak
tiroksine benzeyen lipofilik bir anti-aritmik ajandır.
Waughn-Williams ilaç sınıflandırma sisteminde, sınıf
III potasyum kanal blokeri olarak ventriküler taşikardi
tiplerine ve atriyal fibrilasyona karşı kullanılan ilaçlardan
biridir. Gilaburu (Viburnum opulus L.) dünyada
Avrasya ve Kuzey Afrika’da ormanların çevresinde ve
yurdumuzda en yaygın olarak Kayseri ilinde bulunan
ve tıbbi kullanış amacına sahip olan kırmızı, üzümsü,
bir meyvedir. Gilaburu (GL) içerdiği organik asitlerle,
fenolik bileşiklerle, çeşitli aminoasitlerle, vitaminlerle
yüksek anti-oksidatif, anti-proliferatif, anti-inflamatuvar
etkinliğinin olduğu birçok çalışmada gösterilmiştir.
Çalışmamızda; amiodaronun rat akciğer dokusu üzerinde
oluşturduğu toksisite üzerine gilaburunun koruyucu
etkinliğinin olup olmadığını incelemeyi amaçladık.
Gereç ve Yöntem
Çalışmada 32 adet 300-350 gr ağırlığında erkek Wistar
cinsi rat rastgele seçilerek, Kontrol, AD verilen
grup, AD+GL verilen grup, GL verilen grup şeklinde 4
gruba ayrılmıştır. Deneyde 10 gün süre boyunca ratlara
ip olarak 100 mg/kg AD, oral gavaj yoluyla 100 mg/
kg gilaburu toz meyve ekstraktı serum fizyolojik içinde
çözdürülerek verilmiştir. Deney sonunda akciğer dokuları
alınarak immonuhistokimyasal ve histokimyasal
tekniklerle incelenmiştir.
Bulgular
Yapılan histokimyasal incelemelerde AD grubuna ait
akciğer dokularında oluşan hasarlanmaya karşın,
kombine grupta hasarın azaldığı gözlendi. İmmunohistokimyasal
boyama (TNF-α ve iNOS) sonucunda
da AD grubunda boyanmanın fazla, kombine grupta
boyanmanın daha az olduğu gözlenmiştir.
Sonuç
Sonuç olarak Amiodaronun oluşturduğu akciğer hasarına
karşı gilaburunun koruyucu etkisinin olduğunu
düşünmekteyiz.

Keywords

Amiodaron, Amiodaron Toksisitesi, Akciğer Toksisitesi, Viburnum opulus L.

EFFECT OF GILABURU (Viburnum opulus L.) ON LUNG TOXICITY INDUCED BY AMIODARONE IN RATS

Abstract

Objective
Amiodarone (AD) is a benzofuran derivative, a lipophilic
anti-arrhythmic agent structurally similar to
thyroxine. It is one of the drugs used against ventricular
tachycardia types and atrial fibrillation as a class III
potassium channel blocker in the Waughn-Williams
drug classification system. Gilaburu (Viburnum opulus
L.) is a red, berry-like fruit that is found around the
forests in Eurasia and North Africa in the world and
most commonly in Kayseri province in our country and
has medicinal uses. Many studies has been shown
Gilaburu (GL) to high antioxidative, antiproliferative,
anti-inflammatory activity with the contain organic
acids, phenolic compounds, various amino acids
and vitamins. In our study, we aimed to investigated
whether gilaburu has a protective effect on the toxicity
of amiodarone on rat lung tissue.
Material and Methods
In the study, 32 male Wistar rats weighing 300-350
g were randomly selected and divided 4 groups as,
Control, AD, AD+GL and GL group. In the experiment,
100 mg/kg AD was administered ip to rats for 10 days,
and 100 mg/kg gilaburu powdered fruit extract was
dissolved in physiological saline by oral gavage. At the
end of the experiment, lung tissues were taken and
examined by immunohistochemical and histochemical
techniques.
Results
In the histochemical examinations, it was observed
that the damage occurred in the lung tissues of the
AD group, but the damage decreased in the combined
group. As a result of immunohistochemical staining
(TNF-α and iNOS), it was observed that the staining
was more in the AD group and less in the combined
group.
Conclusion
As a result, we think that gilaburu has a protective
effect against lung damage caused by Amiodarone.

Keywords

Amiodarone, Amiodarone Toxicity, Lung Toxicity, Viburnum opulus L.

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