RATLARDA AMİODARONA BAĞLI AKCİĞER TOKSİSİTESİ ÜZERİNE GİLABURU (Viburnum opulus L.)’NUN ETKİSİ

Abstract

Amaç Amiodaron (AD), benzofuran türevi, yapısal olarak tiroksine benzeyen lipofilik bir anti-aritmik ajandır. Waughn-Williams ilaç sınıflandırma sisteminde, sınıf III potasyum kanal blokeri olarak ventriküler taşikardi tiplerine ve atriyal fibrilasyona karşı kullanılan ilaçlardan biridir. Gilaburu (Viburnum opulus L.) dünyada Avrasya ve Kuzey Afrika’da ormanların çevresinde ve yurdumuzda en yaygın olarak Kayseri ilinde bulunan ve tıbbi kullanış amacına sahip olan kırmızı, üzümsü, bir meyvedir. Gilaburu (GL) içerdiği organik asitlerle, fenolik bileşiklerle, çeşitli aminoasitlerle, vitaminlerle yüksek anti-oksidatif, anti-proliferatif, anti-inflamatuvar etkinliğinin olduğu birçok çalışmada gösterilmiştir. Çalışmamızda; amiodaronun rat akciğer dokusu üzerinde oluşturduğu toksisite üzerine gilaburunun koruyucu etkinliğinin olup olmadığını incelemeyi amaçladık. Gereç ve Yöntem Çalışmada 32 adet 300-350 gr ağırlığında erkek Wistar cinsi rat rastgele seçilerek, Kontrol, AD verilen grup, AD+GL verilen grup, GL verilen grup şeklinde 4 gruba ayrılmıştır. Deneyde 10 gün süre boyunca ratlara ip olarak 100 mg/kg AD, oral gavaj yoluyla 100 mg/ kg gilaburu toz meyve ekstraktı serum fizyolojik içinde çözdürülerek verilmiştir. Deney sonunda akciğer dokuları alınarak immonuhistokimyasal ve histokimyasal tekniklerle incelenmiştir. Bulgular Yapılan histokimyasal incelemelerde AD grubuna ait akciğer dokularında oluşan hasarlanmaya karşın, kombine grupta hasarın azaldığı gözlendi. İmmunohistokimyasal boyama (TNF-α ve iNOS) sonucunda da AD grubunda boyanmanın fazla, kombine grupta boyanmanın daha az olduğu gözlenmiştir. Sonuç Sonuç olarak Amiodaronun oluşturduğu akciğer hasarına karşı gilaburunun koruyucu etkisinin olduğunu düşünmekteyiz.

Keywords

• Amiodaron
• Amiodaron Toksisitesi
• Akciğer Toksisitesi
• Viburnum opulus L.

EFFECT OF GILABURU (Viburnum opulus L.) ON LUNG TOXICITY INDUCED BY AMIODARONE IN RATS

Abstract

Objective Amiodarone (AD) is a benzofuran derivative, a lipophilic anti-arrhythmic agent structurally similar to thyroxine. It is one of the drugs used against ventricular tachycardia types and atrial fibrillation as a class III potassium channel blocker in the Waughn-Williams drug classification system. Gilaburu (Viburnum opulus L.) is a red, berry-like fruit that is found around the forests in Eurasia and North Africa in the world and most commonly in Kayseri province in our country and has medicinal uses. Many studies has been shown Gilaburu (GL) to high antioxidative, antiproliferative, anti-inflammatory activity with the contain organic acids, phenolic compounds, various amino acids and vitamins. In our study, we aimed to investigated whether gilaburu has a protective effect on the toxicity of amiodarone on rat lung tissue. Material and Methods In the study, 32 male Wistar rats weighing 300-350 g were randomly selected and divided 4 groups as, Control, AD, AD+GL and GL group. In the experiment, 100 mg/kg AD was administered ip to rats for 10 days, and 100 mg/kg gilaburu powdered fruit extract was dissolved in physiological saline by oral gavage. At the end of the experiment, lung tissues were taken and examined by immunohistochemical and histochemical techniques. Results In the histochemical examinations, it was observed that the damage occurred in the lung tissues of the AD group, but the damage decreased in the combined group. As a result of immunohistochemical staining (TNF-α and iNOS), it was observed that the staining was more in the AD group and less in the combined group. Conclusion As a result, we think that gilaburu has a protective effect against lung damage caused by Amiodarone.

Keywords

• Amiodarone
• Amiodarone Toxicity
• Lung Toxicity
• Viburnum opulus L.

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