BUĞDAY ÇİMİNİN İNSAN LENFOSİT HÜCRELERİ ÜZERİNE ETKİSİ

Year 2023, , 47 - 55, 14.03.2023

Okan Sancer , Zehra Safi Öz , Pınar Aslan Koşar

https://doi.org/10.17343/sdutfd.1240777

Abstract

Amaç
Kemoterapötik ilaçlar kanser hücrelerinin ortadan
kaldırılmasında etkili iken aynı zamanda sağlıklı hücrelerde
de hasar oluşturabilmektedir. Bu çalışmada,
buğday çimi (Triticum aestivum L.) ekstraktının fenolik
bileşen içeriğinin analizi ve bu ekstraktın kemoterapötik
tedavide kullanılan sisplatin ve etoposid’in sağlıklı
hücrelerde oluşturduğu DNA hasarına karşı etkisinin
belirlenmesi amaçlanmıştır.
Gereç ve Yöntem
Çimlendirilmiş buğdayların metanol ekstraktı hazırlanarak
HPLC (yüksek performanslı sıvı kromatografisi)
ile fenolik bileşen analizi yapıldı. Buğday ekstraktı
konsantrasyonuna bağlı hücre canlılık testi uygulanarak
IC50 (Yarı maksimum inhibitör konsantrasyonu) ve
LD50 (ortalama öldürücü doz) değerleri hesaplandı.
Belirlenen bu konsantrasyon değerleri ile hücreler inkübe
edilerek DNA hasarı varlığı Comet metodu ile
değerlendirildi.
Bulgular
Fenolik bileşen analizi sonucunda p-hidroksibenzoik
asit en yüksek miktarda, o-kumarik asit ise en düşük
düzeyde tespit edildi. Lenfosit hücrelerine uygulanan
farklı konsantrasyonlardaki buğday çimi ekstraktı,
etoposid ve sisplatin için değerler sırasıyla IC50=204,6
μg/mL, LD50=15,84 μg/mL ve LD50=24,51 μg/mL olarak
bulundu. Comet analizi sonucunda kontrol grubuna
kıyasla, etoposid LD50 ve etoposid LD50+buğday
çimi ekstraktı IC50 grubu istatistiksel olarak anlamlı
bulunurken (p<0,05), etoposid LD50 ve etoposid
LD50+buğday çimi ekstraktı IC50 grubu arasında istatistiksel
olarak anlamlılık bulunamadı (p>0,05). Bu
sonuca benzer olarak kontrol grubuna kıyasla, sisplatin
LD50 ve sisplatin LD50+buğday çimi ekstraktı IC50
grubu istatistiksel olarak anlamlı bulunurken (p<0,05),
etoposid LD50 ve etoposid LD50+buğday çimi ekstraktı
IC50 grubu arasında istatistiksel olarak anlamlılık saptanmadı
(p>0,05).
Sonuç
Çalışmamızda buğday çiminin etoposid ve sisplatin
nedeni ile oluşan DNA hasarında azalmaya neden olduğu
görülmüş olmasına rağmen istatistiksel olarak
anlamlılık saptanmamıştır.

Keywords

Buğday çimi, Comet metodu, HPLC, MTT

Thanks

Çalışmamıza ait bazı deneysel aşamaların gerçekleştirilmesinde cihaz kullanılması ile destek olan Süleyman Demirel Üniversitesi Yenilikçi Teknolojiler Uygulama ve Araştırma Merkezi (YETEM)'e teşekkür ederiz.

EFFECT OF WHEATGRASS ON HUMAN LYMPHOCYTE CELLS

Abstract

Objective
While chemotherapeutic drugs are effective at eliminating
cancer cells, they can also damage healthy
cells. The aim of this study was to analyze the phenolic
component content of wheatgrass extract (Triticum
aestivum L.) and to determine the effect of this
extract against DNA damage caused by cisplatin and
etoposide used in chemotherapeutic treatment in
healthy cells will.
Material and Method
Sprouted wheat methanol extract was prepared and
analysis of phenolic components was performed by
HPLC (high performance liquid chromatography).
IC50 (half maximal inhibitory concentration) and LD50
(median lethal dose) values were calculated by applying
a cell viability assay based on wheat extract
concentration. Cells were incubated at these determined
concentration levels and the presence of DNA
damage was assessed by the Comet method.
Results
As a result of the phenol component analysis, p-hydroxybenzoic
acid was determined in the highest
amount and o-coumaric acid in the lowest amount.
The values for wheatgrass extract, etoposide and
cisplatin at different concentrations applied to lymphocyte
cells were found to be IC50=204.6 μg/mL,
LD50=15.84 μg/mL and LD50=24.51 μg/mL, respectively.
As a result of comet analysis, it was found
that etoposide LD50 and etoposide LD50+wheatgrass
extract IC50 group were statistically significant
(p<0.05), compared to the control group, there was
no statistical significance between etoposide LD50
and etoposide LD50+wheatgrass extract IC50 group
(p>0.05). Similar to this result, cisplatin LD50 and
cisplatin LD50+wheatgrass extract IC50 group were
found to be statistically significant compared to the
control group (p<0.05), while there was no statistical
significance between etoposide LD50 and etoposide
LD50+wheatgrass extract IC50 group (p> 0.05).
Conclusion
Although wheatgrass was observed in our study to
cause a reduction in DNA damage caused by etoposide
and cisplatin, no statistical significance was
found.

Keywords

Wheatgrass, Comet method, HPLC, MTT

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