RİFAMPİSİN KAYNAKLI BEYİN DOKUSU HASARINDA LİNALOOL’UN İYİLEŞTİRİCİ RÖLÜ

Year 2023, , 362 - 370, 23.09.2023

Meltem Özgöçmen , Sebile Azırak

https://doi.org/10.17343/sdutfd.1282668

Abstract

Objective
In this study linalool (LN), which has antihyperglycemic,
hypolipidemic and antioxidant properties, is intended
to be used in the treatment of neurodegenerations
and neural disorders that may occur due to rifampicin
(RF). For this reason, it was aimed to examine the
effects of LN on the expression of genes, biochemical
and histopathological parameters in these metabolic
pathways against neurotoxicity that may occur due
to RF, and to investigate the protective effects of LN,
which has antioxidant properties.
Material and Method
Thirty healthy male Spraque-Dawley rats were divided
into five groups (group 1; control, group 2; solvent
control (DMSO); group 3, RF; group 4, LN; group 5;
RF+LN). Brain tissues were taken for biochemical,
histological and gene expressions analyses. Blood
samples were taken to measure blood glucose levels.
Results
Rifampicin treatment significantly increased CYP1A1
and CYP1A2 mRNA gene expression and blood
glucose levels, while reducing brain weight according
to findings. On the other hand, there was a significant
decrease in CYP1A1 and CYP1A2 mRNA gene
expression and blood glucose levels in the RF+LN
group, while a significant improvement in brain weight
was observed and as a result of histological analyzes,
it was observed that the damage caused by RF
decreased in the groups given LN.
Conclusion
LN was found to be highly effective in protecting the
brain from the toxic effects of RF.

Keywords

CYP1A2, Linalool, Brain, CYP1A1, Rifampicin.

THE CURATIVE ROLE OF LINALOOL IN RIFAMPICIN-INDUCED BRAIN TISSUE DAMAGE

Abstract

Amaç
Bu çalışmada antihiperglisemik, hipolipidemik ve antioksidan
özelliklere sahip olan linalool (LN), rifampisine
(RF) bağlı olarak oluşabilen nörodejenerasyonlar
ve nöral bozuklukların tedavisi amacıyla kullanılmıştır.
Bu amaçla, RF'ye bağlı oluşabilecek nörotoksisitede,
antioksidan özelliği olan LN'nin koruyucu etkinliği hem
gen ekspresyonu seviyesinde hem de biyokimyasal
ve histopatolojik bulgularla araştırılmıştır.
Gereç ve Yöntem
30 adet sağlıklı erkek Spraque-Dawley rat beş gruba
ayrıldı (Grup 1: Kontrol grubu; Grup 2: Çözücü Kontrol
(Dimetilsülfoksit-DMSO) grubu; Grup 3: Rifampisin
(RF) grubu; Grup 4: Linalool (LN) grubu; Grup 5:
Rifampisin-Linalool (RF+LN) grubu. Biyokimyasal ve
histolojik analizler ile gen ekspresyon seviyeleri için
beyin dokuları, kan glikoz seviyelerini ölçmek için kan
örnekleri alındı.
Bulgular
Rifampisin uygulaması, beyin ağırlığını azaltırken,
CYP1A1 ve CYP1A2 mRNA gen ekspresyonunu ve
kan glukoz düzeylerini önemli ölçüde artırmıştır. Diğer
yandan RF+LN grubunda CYP1A1 ve CYP1A2 mRNA
gen ekspresyonu ve kan glukoz düzeylerinde anlamlı
azalmalar olurken, beyin ağırlığında anlamlı düzelme
gözlenmiştir. Histolojik bulgular LN verilen gruplarda,
RF ye bağlı oluşan hasarların azaldığını göstermiştir.
Sonuç
LN'nin beyin dokusunu RF'nin toksik etkilerinden korumada
oldukça etkili olduğu tespit edilmiştir.

Keywords

CYP1A2, Linalool, Rifampisin, Beyin, CYP1A1

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