Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi

Recep Kutlubay; Emin Oğuz; Günfer Turgut; Erdoğan Kocamaz

doi:10.17343/sdutfd.14900

Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi

Abstract

Süleyman Demirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 2008 Aralık; 15 (4) Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi Recep Kutlubay*, Emin Oğuzhan Oğuz*, Günfer Turgut**, Erdoğan Kocamaz*, Özet Amaç: Histolojik olarak etanolün belli dozda karaciğer ve böbrekte yarattığı hasar ve bu hasarı önlemek üzere verilen L-NAME.in muhtemel koruyucu etkisinin gösterilmesi. Gereç ve yöntem: Çalışmada 6 aylık Wistar erkek sıçanlar kullanılmıştır. Sıçanlar rastgele 10.arlı 4 gruba ayrılmışlardır: I- Kontrol (n=10) II-Etanol (n=10) III- L-NAME (n=10) IV-Etanol+ L-NAME (n=10) L-NAME litrede 0.1 gram olacak şekilde, etanol ise hacmen %20 olacak şekilde yukarıda belirtilen gruptaki sıçanların içme sularına karıştırılarak 30 gün boyunca verilmiştir. Bulgu ve sonuçlar: Deney sonucu alınan kimysallara bağlı olarak herhangi bir hayvan ölümü gözlenmedi. Böbrek.te etanol grubunda Bowman boşluklarının daraldığı, tübül hücrelerinin yer yer belirgin vakuolleşmeler gösterdiği, tübül epiteline ait hücre çekirdeklerinin patolojik görüntüler sergilediği görüldü. Etanolle + L-NAME grubunda Bowman boşlularının kontrollerdekine benzer şekilde normal genişlikte olduğu, tübüllerin hemen hemen tamamına yakını normal olduğu görüldü. Sadece L- NAME uygulanan gruptaki glomeruluslar ve tübüler yapılar kontrollerdekinden faklılık göstermedi Karaciğer.de Etanol grubunun genel görüntülerinde sinüzoidler belirgin şekilde daralmıştı. Hepatosit kordonları yer yer düzensiz hücre yığınları halinde olduğu, sinüzoidlerin büyük oranda ortadan kalktığı, belirlendi. Ayrıca bazı hepatositler dejeneratif görüntüler sergilemekteydi. Etanol + L-NAME grubunda sinüzoidler nomal görünümlüydü. Lobül periferinde çok küçük bir bölgede hafif düzensizlikler olmasına rağmen hepatosit kordonları normal görünümdeydi. Sadece L-NAME uygulanan grupta sinüzoidlerin, hepatosit kordonlarının ve hepatosit sitoplazmalarının kontrollerdekinden belirgin bir farklılık göstermediği belirlendi. Bu bulgulara göre karaciğer ve böbrek dokularında etanolün kendi başına yarattığı yapısal hasarın L-NAME ile birlikte verildiğinde büyük oran da azaldığı gözlendi. Anahtar kelimeler: Etanol, L-NAME, karaciğer, böbrek, toksisite, ışık mikroskobu Abstract The toxic effect of etanol on liver and kidney and the protective effect of L-NAME Aim: To show the histological liver and renal damage caused by ethanol at a certain dose and to show possible protective role of L-NAME. Materials and Methods: In this study, 40, 6 month-old Wistar rats were randomly divided into four groups: I- Control (n=10) II- Etanol (n=10) III-L-NAME (n=10) IV-Etanhol + L-NAME (n=10) 1g/lt L-NAME and 20% etanol were added into drinking water for 30 days. Results: At the end of the experiment, no animal died to due the given agents. In kidney, in ethanol group, narrowing in Bowman spaces, vacuolisation and pathologic appearance in tubule epithelium were observed. Ethanol + L-NAME group showed normal Bowman spaces similar in control group and almost normal tubuli structure. Only L-NAME group showed normal appearance as it was in control group in terms of glomerular and tubular structures.In liver, in ethanol group there was significant narrowing in sinusoids, in some places irregular hepatocyte accumulations, degenrative

Keywords

Etanol, L-NAME, karaciðer, böbrek, toksisite, ýþýk mikroskobu

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Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Recep Kutlubay This is me

Emin Oğuz This is me

Günfer Turgut This is me

Erdoğan Kocamaz This is me

Publication Date

May 29, 2009

Submission Date

May 28, 2009

Acceptance Date

-

Published in Issue

Year 2008 Volume: 15 Number: 4

DOI

https://doi.org/10.17343/sdutfd.14900

IZ

https://izlik.org/JA26LF63GT

Cite

RIS / Bibtex

APA

Kutlubay, R., Oğuz, E., Turgut, G., & Kocamaz, E. (2009). Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi. Medical Journal of Süleyman Demirel University, 15(4), 11-17. https://doi.org/10.17343/sdutfd.14900

AMA

1.Kutlubay R, Oğuz E, Turgut G, Kocamaz E. Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi. Med J SDU. 2009;15(4):11-17. doi:10.17343/sdutfd.14900

Chicago

Kutlubay, Recep, Emin Oğuz, Günfer Turgut, and Erdoğan Kocamaz. 2009. “Karaciğer Ve Böbrek üzerine Etanolün Toksisitesi Ve L-NAME. In Koruyucu Etkisi”. Medical Journal of Süleyman Demirel University 15 (4): 11-17. https://doi.org/10.17343/sdutfd.14900.

EndNote

Kutlubay R, Oğuz E, Turgut G, Kocamaz E (May 1, 2009) Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi. Medical Journal of Süleyman Demirel University 15 4 11–17.

IEEE

[1]R. Kutlubay, E. Oğuz, G. Turgut, and E. Kocamaz, “Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi”, Med J SDU, vol. 15, no. 4, pp. 11–17, May 2009, doi: 10.17343/sdutfd.14900.

ISNAD

Kutlubay, Recep - Oğuz, Emin - Turgut, Günfer - Kocamaz, Erdoğan. “Karaciğer Ve Böbrek üzerine Etanolün Toksisitesi Ve L-NAME. In Koruyucu Etkisi”. Medical Journal of Süleyman Demirel University 15/4 (May 1, 2009): 11-17. https://doi.org/10.17343/sdutfd.14900.

JAMA

1.Kutlubay R, Oğuz E, Turgut G, Kocamaz E. Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi. Med J SDU. 2009;15:11–17.

MLA

Kutlubay, Recep, et al. “Karaciğer Ve Böbrek üzerine Etanolün Toksisitesi Ve L-NAME. In Koruyucu Etkisi”. Medical Journal of Süleyman Demirel University, vol. 15, no. 4, May 2009, pp. 11-17, doi:10.17343/sdutfd.14900.

Vancouver

1.Recep Kutlubay, Emin Oğuz, Günfer Turgut, Erdoğan Kocamaz. Karaciğer ve böbrek üzerine etanolün toksisitesi ve L-NAME. in koruyucu etkisi. Med J SDU. 2009 May 1;15(4):11-7. doi:10.17343/sdutfd.14900