Biyolojik damarsal greft üretiminde hücresizleştirme metodları; güncel literatür derlemesi

Abstract

Hücresizleştirme (Decellularization) hücre ve hücre içi organellerin zarlarının

bütünlüğünü bozarak DNA, RNA, yağlar, mitokondri ve sitoplazmik zardaki hücre

kimliğini taşıyan proteinler ve sitozolik içerik gibi tüm hücresel yapıların temizlenerek,

önemli hücre dışı matriks yapılarının üç boyutlu ultrastrüktür, temel matriks proteinleri

ve büyüme faktörleri gibi öğelerinin korunması ve sonrasında implantasyon ile

in vivo ya da in vitro ortamda dokunun kendine ait özelliklerle başarılı bir şekilde

yeniden modellenmesi işlemidir. Bu derleme makalesinin amacı damar dokusunun

hücresizleştirilmesi işlemi sırasında kullanılan birçok farklı çeşit yöntemin incelenerek

başarılı veya başarısız yönlerinin ortaya konmasıdır.

Keywords

• Hücresizleştirme,doku,yapay organ

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