Gundelia tournefortii L. Bitki Ekstraktlarının Antimikrobiyal Aktivitelerinin ve İn Vitro Sitotoksik Aktivitelerinin Değerlendirilmesi

Abstract

Giriş: Kenger, Asteraceae familyasından Gundelia tournefortii bitkisi, tıbbi bir bitkidir. Kenger'in çiçeklerinin, yapraklarının, tohumlarının ve köklerinin besin kaynağı olarak kullanıldığı bilinmektedir.

Materyal ve method:Bu çalışmada Sivas ilinde, taze filizleri soyularak çiğ ve pişirilerek yenilen Kenger’in gövde kısımlarının su fazı ve uçucu yağ ekstraksiyonu yapılarak, özütlerin antimikrobiyal ve in vitro antiproliferatif özellikleri bakımından değerlendirilmesi amaçlanmıştır. Sitotoksik aktiviteleri Meme Kanseri Hücre Hattı (MCF-7) ve insan endotelyal hücre hattı (HUVEC) kullanılarak araştırılmıştır. Bitki özütlerinin hücre kültürlerindeki etkileri, XTT yöntemiyle Eliza reader cihazında absorbans bulunarak elde edilmiştir. Kenger bitki özütlerinin MCF-7 hücreleri ve endotelyal hücreler üzerinde sitotoksik etkileri belirlenmiştir. 

Bulgular:Kenger bitki özütlerinin zayıf antimikrobiyal etkilerinin olduğu MIC değerleriyle ortaya konulmuştur.  

Sonuç:Sivas ilinde halk arasında şifalı olduğu bilinen, bahar ve yaz aylarında büyüyen bitki kısımları kesilip soyularak iç kısımlarının besin olarak tüketildiği, çeşitli hastalıkların tedavisinde kullanılan Kenger bitkisinin, denediğimiz hücreler üzerinde sitotoksik etkilerinin olduğu, zayıf antimikrobiyal etkisinin bulunduğu görülmektedir.   

Keywords

• Kenger,antimikrobiyal,antikanserojen,ekstraksiyon

Evaluation of Antimicrobial Activities and In Vitro Cytotoxic Activities of Gundelia tournefortii L. Plant Extracts

Abstract

Introduction:Kenger, Gundelia tournefortiiplant from Asteraceae family, is a medicinal plant. Flowers, leaves, seeds and roots of Kenger have been reported to be used as a source of food. 

Material ve methods:In this study, it was aimed to evaluate the antimicrobial and in vitroantiproliferative properties of Kenger which fresh sprouts are peeled and eated cooked or uncooked in Sivas. The water phase and essential oil extraction of the body parts of Kenger were aimed to be evaluated in terms of their antimicrobial and in vitroantiproliferative properties.

Results:Cytotoxic activities were investigated using the Breast Cancer Cell Line (MCF-7) and human endothelial cell line (HUVEC). The effects of plant extracts on cell cultures were determined by the absorbance of Eliza reader by XTT method. Kenger plant extracts have been shown to have weak antimicrobial effects with MIC values. 

Conclusions:In the province of Sivas, growing parts of the plant in the spring and summer are cut and peeled into the inner parts of the nutrients. Kenger, which is used in the treatment of various diseases, has cytotoxic effects on the cells we tested and has a weak antimicrobial effect.

Keywords

• Kenger,antimicrobial,anticarcinogen,extraction

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