THE EFFECTS OF IMMOBILIZATION STRESS ON PLACENTA AND FETUS IN PREGNANT MICE

Year 2021, , 31 - 39, 11.03.2021

Nihan Semerci Gökçen Bilici Filiz Yılmaz , Zahide Çavdar Uygar Sacık Ümit Kayışlı Guven Erbil

https://doi.org/10.17343/sdutfd.651091

Abstract

ABSTRACT

 

AIM: Stress can affect negatively mother and fetuses during
pregnancy. We aimed to investigate the effects of chronic immobilization stress
on placental maturation and fetal development.

MATERİALS AND METHODS: Balb/c virgin female mice (20-30 g)
were mated with male mice in a 2 to 1 female to male ratio. Pregnant mice in
control group (n=6) were left undisturbed, whereas pregnant mice in the stress
group (n=6) were exposed to 45 min chronic immobilization stress for three
times/day starting from gestational day 6 till 18. Fetuses and placentas were
removed from dams on the gestational day 18 under anesthesia.

RESULTS: The prenatal stress significantly
increased apoptosis in several placental cells including trophoblastic giant
cells, glycogen cells and labyrinth trophoblastic cells and resulted in
intrauterine growth restriction. The stress caused a decreased superoxide
dismutase and glutathione levels. Alizarin Red S staining shows the
ossification center of the fetuses to see developmental abnormality.

CONCLUSION: Gestational stress causes placental
dysfunctions by triggering apoptosis, reducing the labyrinth zone as well as
increasing collagen levels, which may impair fetal development that may
contribute to pathogenesis of intrauterine growth restriction.

Keywords

fetus, immobilization stress, placenta

Supporting Institution

DEU Scientific Research Projects Department Directorate

Project Number

2012.SAG.BİL.073

FARE GEBELİK DÖNEMİNDE HAREKETSİZLİK STRESİNİN PLASENTA VE YAVRUYA ETKİLERİ

Abstract

ÖZ

 

AMAÇ: Stres gebelik sürecinde anne ve
fetüs sağlığını olumsuz etkilemektedir. Çalışmamızda kronik hareketsizlik
stresinin plasenta ve fetüs gelişimi üzerine etkilerini araştırmayı hedefledik.

GEREÇ VE YÖNTEMLER: Balb/c
suşu dişi fareler (20-30gr), 2dişi-1erkek olacak şekilde katıma alındı. Kontrol
grubundaki (n=6) gebe farelere herhangi bir uygulama yapılmazken, stres
grubundaki (n=6) gebe farelere gebeliğin 6.gününden 18.gününe kadar günde 3
defa 45dakikalık kronik hareketsizlik stresine maruz bırakıldı. Gebeliğinin 18.
Gününde plasenta ve fetüsler anestezi altında sezaryen ile alındı.

BULGULAR: Prenatal
stres, trofoblastik dev hücreler, glikojen içeren hücreler ve labirent
trofoblastik hücreler dahil olmak üzere birçok plasental hücrede apoptozu
önemli ölçüde arttırdı ve intrauterin büyüme geriliğine sebep oldu. Stres
süperoksit dismutaz ve glutatyon seviyelerini azalttı. Fetüsün gelişimini
değerlendirmek için, Alizarin Red S boyaması ile fetüsün kemikleşme merkezleri değerlendirildi.

SONUÇ: Gebelik sürecindeki stres,
apoptozu tetikleyerek, labirent bölgesi küçüldü ve plasenta yetmezliğine sebep
oldu, ayrıca kollajen seviyelerini arttırarak fetüs gelişimini olumsuz yönde
etkileyerek intrauterin büyüme geriliği patogenezinde katkısı olduğunu
gözlemledik.

Keywords

Fetus, hareketsizlik stresi, plasenta

Project Number

2012.SAG.BİL.073

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