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Comparison of polimerase chain reaction and serological methods in the diagnosis of hepatitis C virus infection

Year 2007, Volume: 14 Issue: 1, 10 - 14, 30.04.2009

Abstract

SüleymanDemirel Üniversitesi
TIP FAKÜLTESİ DERGİSİ: 2007 Mart; 14(1)










Comparison of polimerase chain reaction and serological methods in the diagnosis of hepatitis C virus infection



Selçuk Kaya, Buket Cicioğlu-Arıdoğan, Emel Sesli Çetin, Ali K. Adiloğlu, Mustafa Demirci



Süleyman Demirel University Medical School Medical Microbiology Department, Isparta, Turkey.







Özet



Hepatit C Virus infeksiyonu tanısında polimeraz zincir reaksiyonu ve serolojik yöntemlerinin karşılaştırılması



Hepatit C virus (HCV) infeksiyonunun tanısı, serolojik olarak anti-HCV veya moleküler yöntemlerle HCV RNA'nın tespitiyle gerçekleştirilebilmektedir. Bu çalışmada HCV infeksiyonu tanısında polimeraz zincir reaksiyonu ve serolojik yöntemlerinin yerinin araştırılması amaçlanmıştır. Çalışmaya 87 hasta alındı. HCV RNA real time PCR (GeneAmp 5700 Sequence Detection System) ile tespit edildi. Seksenyedi hastanın 30 (%34.5)'unda HCV RNA ve 60 (%69)'ında anti-HCV pozitifliği tespit edildi. HCV antikorları pozitif hastaların 14'ünde antikor titreleri 2.5 U/ml'nin altında bulundu. Düşük titreli hastaların hiçbirinde HCV RNA pozitifliği saptanmadı. Antikoru negatif hastaların hepsinin HCV RNA'ları da negatifti. Ayrıca HCV RNA pozitif hastaların hepsinin HCV antikorları da pozitifti. HCV antikorları negatif tüm hastalarda HCV RNA'nın da negatif olması göz önünde bulundurulursa rutin tarama amaçlı işlemlerde güvenilir bir ELISA tekniği ile anti-HCV antikorunun araştırılmasının kişinin HCV virusu ile infekte olup olmadığını belirlemede yeterli olacağı düşünülebilir. Diğer yandan anti-HCV antikorları tespit edilen serumların öncelikle ELISA ile tekrar çalışılması ve hastalığın doğrulanması için HCV RNA testlerinin yapılması gerektiği düşünülmektedir. ELISA ve PCR metodlarının birlikte kullanılması pozitif sonuçların doğrulanmasına ve erken tanıya yardımcı olacaktır.



Anahtar kelimeler: Hepatit C virus, polimeraz zincir reaksiyonu, anti-HCV





Abstract





Diagnosis of HCV infection depends on detecting anti-HCV antibody (HCV-Ab) through serologic methods or on detecting of HCV RNA with molecular methods. The aim of this study was to evaluate the use of serological methods and polymerase chain reaction (PCR) for the diagnosis of HCV infection. A total of 87 patients were studied. HCV RNA was detected by real time PCR with GeneAmp 5700 Sequence Detection System. Thirty (34.5%) of 87 patients were HCV RNA positive and 60 (69%) were HCV-Ab positive. Fourteen of the HCV-Ab positive patients had low antibody titers (below 2.5 U/ml). None of these was found as HCV RNA positive. All of HCV-Ab negative patients were HCV RNA negative and all of the HCV RNA positive cases were HCV-Ab positive also. Since HCV RNA was negative in all of HCV Ab negative patients, it can be suggested that HCV-Ab detection by a reliable ELISA technique in the routine procedure is sufficient to determine HCV infection . On the other hand, low titer positive HCV-Ab cases must be restudied by ELISA, and PCR for HCV RNA confirmation. By using ELISA and PCR methods at the same time would help to confirm the positive results and early diagnosis.



Key words: Hepatitis C virus, polimerase chain reaction, anti-HCV

References

  • Anand BS, Velez M. Assessment of correlation between serum titers of hepatitis c virus and severity of liver disease. World J Gastroenterol 2004;10(16):2409- 1.
  • A lter MJ. The epidemiology of hepatitis C virus in the est. Semin Liv Dis 1995; 15: 5-14.
  • C astro FC, Sauleda S, Esteban JI, Viladomiu L, Martell , Dragon E, Esteban R and Guardia J. Evaluation of epatitis C virus RNA RT/PCR qualitative and uantitative second generation assays. J Virol Meth
  • de Medina M, Hill M, Sullivan HO, Leclerq B, Pennell JP, Jeffers L, Reddy KR, Schiff ER, Perez GO. Detection of anti-hepatitis C virus antibodies in patients undergoing dialysis by utilizing a hepatitis C virus 3.0 assay: correlation with hepatitis C virus RNA. J Lab Clin Med. 1998 Jul;132(1):73-5.
  • Takeuchi T, Katsume A, Tanaka T, Abe A, Inoue K, Tsukiyama-Kohara K, Kawaguchi R, Tanaka S, Kohara M. Real-time detection system for quantification of hepatitis C virus genome. Gastroenterology. 1999;116(3):763-4.
  • Shigeki M, Kayoko F, Chieko M, Rieko S, Shinichi H, Kazunori N, Kenji T and Takeo J. High-throughput HBV DNA and HCV RNA detection system using a nucleic acid purification robot and real-time detection PCR: its application to analysis of posttransfusion hepatitis. Transfusion2002;42(1):100-6.
  • Sayan M, Meriç M, Mutlu B, Çelebi S, Wilke A. Low positive anti-HCV microparticle enzyme immunoassay results: do they predict hepatitis C virus infection? Mikrobiyoloji Bülteni 2006;40:81-4.
Year 2007, Volume: 14 Issue: 1, 10 - 14, 30.04.2009

Abstract

References

  • Anand BS, Velez M. Assessment of correlation between serum titers of hepatitis c virus and severity of liver disease. World J Gastroenterol 2004;10(16):2409- 1.
  • A lter MJ. The epidemiology of hepatitis C virus in the est. Semin Liv Dis 1995; 15: 5-14.
  • C astro FC, Sauleda S, Esteban JI, Viladomiu L, Martell , Dragon E, Esteban R and Guardia J. Evaluation of epatitis C virus RNA RT/PCR qualitative and uantitative second generation assays. J Virol Meth
  • de Medina M, Hill M, Sullivan HO, Leclerq B, Pennell JP, Jeffers L, Reddy KR, Schiff ER, Perez GO. Detection of anti-hepatitis C virus antibodies in patients undergoing dialysis by utilizing a hepatitis C virus 3.0 assay: correlation with hepatitis C virus RNA. J Lab Clin Med. 1998 Jul;132(1):73-5.
  • Takeuchi T, Katsume A, Tanaka T, Abe A, Inoue K, Tsukiyama-Kohara K, Kawaguchi R, Tanaka S, Kohara M. Real-time detection system for quantification of hepatitis C virus genome. Gastroenterology. 1999;116(3):763-4.
  • Shigeki M, Kayoko F, Chieko M, Rieko S, Shinichi H, Kazunori N, Kenji T and Takeo J. High-throughput HBV DNA and HCV RNA detection system using a nucleic acid purification robot and real-time detection PCR: its application to analysis of posttransfusion hepatitis. Transfusion2002;42(1):100-6.
  • Sayan M, Meriç M, Mutlu B, Çelebi S, Wilke A. Low positive anti-HCV microparticle enzyme immunoassay results: do they predict hepatitis C virus infection? Mikrobiyoloji Bülteni 2006;40:81-4.
There are 7 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Selçuk Kaya

Buket Arıdoğan This is me

Emel Çetin This is me

Ali K. Adiloğlu This is me

Mustafa Demirci This is me

Publication Date April 30, 2009
Submission Date April 30, 2009
Published in Issue Year 2007 Volume: 14 Issue: 1

Cite

Vancouver Kaya S, Arıdoğan B, Çetin E, Adiloğlu AK, Demirci M. Comparison of polimerase chain reaction and serological methods in the diagnosis of hepatitis C virus infection. Med J SDU. 2009;14(1):10-4.

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