Gebelik esnasýnda diazinon’a maruz kalan ratlarýn yenidoðan yavrularýnýn akciðer dokusunda oluþan morfolojik deðiþiklikler ve bu deðiþikliklere kafeik asit fenetil ester’in etkisinin araþtýrýlmasý

Year 2011, Volume: 18 Issue: 1, 6 - 11, 30.03.2011

Osman Sulak , Seyhan Çankaya , Gülnur Özgüner , Orhan Baş , Önder Şahin Ahmet Songur

Abstract

Objectives: The aim of present study is to investigate morphological changes occurred in lungs of newborn rats whose mothers exposed to diazinon during their pregnancy, and effects of CAPE on these changes. Methods: Thirty female Wistar albino rats were randomly divided into three groups, as follows: Group I(n; 10): control group, Group II (n; 10): 25mg/kg/day diazinon treated group in the 14-18 days of pregnancy, Group III (n; 10): 25 mg/kg/day diazinon + 10 µmol /kg/day CAPE treated group in the 14-18 days of pregnancy. Diazinon administrated by oral gavage in corn oil and CAPE administrated intraperitoneally. Results: In group II, diazinon causes low weight gain in pregnant rats and low birth weights in their newborns. Histopathological examinations of lung tissues revealed intraparenchymal inflammatory infiltration, alveolar and bronchial hemoraji, intraparenchymal vascular congestion and thrombozis and emphysematous changes. In group III, administration of CAPE has considerably reduced these pathological changes. As a result, low weightgain in pregnant rats and low birth weights in their newborns also, histopathological changes in the lung tissues of newborn rats determined. It is also concluded that CAPE has ameliorating effects on these histopathological changes.

Keywords

Diazinon, CAPE, histopathology, lung, rat

Gebelik esnasında diazinon'a maruz kalan gebe ratların yenidoğan yavrularının akciğer dokusunda oluşan morfolojik değişiklikler ve bu değişikliklere kafeik asit fenetil ester'in etkisinin araştırılması

Abstract

Amaç: çalışmanın amacı gebelikte diazinon'a maruz kalan bebe ratların yenidoğan yavrularının akciğer dokusunda oluşan morfolojik değişiklikleri ve bu değişikliklere kafeik asit fenetil ester'in (CAPE) nin etkisinin araştırılmasıdır. Gereç ve Yöntem: Bu çalışmada 30 Wistar albino rat kullanıldı. Ratlar rastgele 3 gruba ayrıldı. Grup 1: kontrol grubu (n;10), grup 2 (n;10): Gebeliğin 14-18. günlerinde 25 mg/kg/gün diazinon verilen grup, grup 3 (n;10): Gebeliğin 14-18. günlerinde 25 mg/kg/gün diazinon + 10 µmol /kg/day CAPE verilen grup. Deney gruplarında diazinon oral gavaj ile CAPE ise intraperitoneal uygulandı. Gebe ratların gebelik boyunca kilo alımları ve yenidoğan yavruların doğum ağırlıkları takip edildi. Yenidoğan yavrular 3. günde dekapite edildi. Akciğerleri diseke edilerek %10'luk formaldehid içerisine alınarak rutin histalojik doku takibi yapıldı ve preperatlar ışık mikoskobunda değerlendirildi. Bulgular: Grup 2'deki anne ratların ve yenidoğanlarının ortalama kiloları kontrol grubuna göre daha düşüktü. Ayrıca grup 2'deki yenidoğanların akciğerlerinde intraparankimal inflamatuar infiltrasyon, alveolar ve bronşiolar hemoraji, intraparankimal vasküler konjesyon ve trombosis, ve amfizematöz değişiklikler gözlendi. Diazinon ve CAPE'nn birlikte verildiği grup 3'te ise bu patolojilerin önemli oranda azaldığı tespit edildi. Sonuç olarak gebelikte diazinon'a maruz kalan gebe ratların kilo alımlarında ve yeni doğanlarının kilolarında azalma ayrıca akciğer dokularında histopatolojik değişiklikler tespit edildi ve CAPE uygulanması ile bu değişikliklerin önemli derecede azaldığı gözlendi.

Keywords

Diazinon, CAPE, histopatoloji, akciðer, rat

References

• Roegge CS, Timofeeva OA, Seidler FJ, Slotkin TA, Levin ED. Developmental diazinon neurotoxicity in rats: Later effects of on emotional response. Brain Research Bulletin 2008; 75: 166-72
• Abdollahi M, Mostafalou S, Pournourmohammadi, Shadnia S. Oxidative stres and cholinesterase inhibition in saliva and plasma of rats following subchronic exposure to malathion. Comp Biochem Physiol C Toxicol Pharmacol 2004; 137:29-34
• John S, Kale m, Rathore N, Bhatnagar D. Protective effect of Vitamin E in dimethoate and malathion induced oxidative stres in rat erytrocytes. J Nutr Biochem 2001;12: 500-04
• Carr RL, Richardson JR, Guarisco JA, Kachroo A, Chambers JE, Couch TA et al. Effects of PBC exposure on the toxic impact of organophosphors insectised. Toxicol Sciences 2002; 67: 311-21
• Zendzian RP. Pesticide residue on/in the washed skin and its potential contribution to dermal toxicity. J Appl Toxicol 2003; 23: 121-36
• Muggleton NG, Smith AJ, Scott EA, Wilson SJ, Pearce PC. Along-term study of effects of diazinon on sleep, the electrocorticogram and conitive behaviour in common marmosts. Psychopharmacol 2005; 19:455- 66
• Stephan B, Kyle L, Yong X, Cynthia A, Donald E, Earl F, James E. Role of oxidative stres in the mechanism of dieldrins hepatotoxicity. Ann Clin Lab Sci 1997; 27:196-208
• Handy RD, Abd-El Samei HA, Bayomy MFF, Mahran AM, Abdeen AM, El –Elaimy EA. Chronic diazinon exposure: pathologies of spleen, thymus, blood cells and lymph nodes are modulated by dietary protein or lipid in the mouse. Toxicology 2001; 172: 13-34
• Rodrigo L, Hernandez AF, Lopez-Caballero JJ, Gil F, Pla A. Chem. Biol. Interact 2001; 137: 123137
• Joshi SC, Mathur R, Gajra A, Sharma T. In fluence of methyl parathion on reproductive parameters on male rats. Environ Toxicol Pharmacol 2003; 14: 91- 8
• Whyatt RM, Rauh V, Barr DB, Camann DE, Andrew HF, Garfinkel R et all. Prenatal insecticide exposures and birth weight and 3ength among an Urban Minorty Cohort. Environ Health Persp 2004; 112: 1125- 32
• Gökçimen A, Gülle K, Demirin H, Bayram D, Koçak A, Altuntas Ý. Effects of diazinon at different doses on rat liver and pancreas tissues. Pestic Biochem Phys 2007; 87: 103- 08
• Kalender S, Ogutcu A, Uzunhisarcýklý M, Açýkgöz F, Durak D, Ulusoy Y, Kalender Y. Diazinon – induced hepatotoxicity and protective effect of vitamin E on some biochemical indices and ultrastructural changes. Toxicology 2005; 211: 197- 206
• Fesen MR, Pommier Y, Leteurtre E, Hiroguchi S, Yung J, Kohn KW. Inhibition of HIV-1 integrase by flavones, caffeic acid phenethyl ester (CAPE) and related compouns. Biochem Pharmacol 1994; 48: 595- 608
• Rao C, Desai D, Kaul B, Amin S, Reddy BS. Effect of caffeic acid esters on carcinogen-nduced mutagenicity ond human colon adenocarsinoma cel growth. Chem Biol Interact 1992; 84: 277- 90
• Sud’ina GF, Mirzoeva OK, Pushkareva GA, Korshunova GA, Sumbatyan NV, Varfolomeev SD. Caffeic acid phenethyl ester as a lipoygenase inhibitor with antioxidant properties. FEBS Lett 1993; 328: 21- 4
• Chen YJ, Shiao MS, Wang SY. The antioxidant caffeic acid phenethyl ester induces apoptosis associated with selective scavenging of hydrogen peroxide in human leukemic HL- 60 cells. Anticanser Drugs 2001; 12: 143- 49
• Michaluart P, Masfererr JL, Carothers AM, Subbaramaiah K, Zweifel BS, Koboldt C, et al. Inhibitory effects of caffeic acid phenethyl ester on the activity and expression of cyclooxygenase-2 in human oral epithelial cells and in a rat model of iflammation. Cancer Res 1999; 59: 2347- 52
• Ýlhan A, Koltuksuz U, Ozen S, Uz E, Çiralik H, Akyol O. The effects of caffeic acid phenethyl ester (CAPE) on spinal cord ischemia/ reperfusion injury in rabbits. Eur. J. Cardiothorac. Surg 1999; 1: 458- 63
• Þahin O, Sulak O, Yavuz Y, Uz E, Eren Ý, Yýlmaz HR ve ark. Lithium-induced lung toxicity in rat. The effect of caffeic acid phenethyl ester(CAPE). Pathology 2006; 38: 58- 62
• Özyurt H, Söðüt S, Yýldýrým Z, Kart L, Iraz M, Armutcu F ve ark. Inhibitory effect of caffeic acid phenethyl ester on bleomycine-induced lung fibrosis in rats. Clin Chim Acta 2004; 339: 65- 75
• Fidan F, Ünlü M, Sezer M, Þahin Ö, Tokyol C, Esme H. Acute effects of enviromental tobacco smoke and dried dung smoke on lung histopathology in rabbits. Pathology 2006; 38: 53- 7
• Isparta Tarým Ýl Müdürlüðü 2004 yýlýna ait yayýmlanan meyve zararlarý ile mücadele raporu. Tasdik eden Onur Çarýkcý 2005
• Eskenazi B, Harley K, Bradman A, Weltzien E, Jewell NP, Barr DB, et al. Association of in utero organohoshate pesticide exposure and fetal growth and length of gestation in an agricultural population. Environ Health Perspect. 2004; 112:1116- 24
• Dabrowski S, Hanke W, Polanska K, Makowiec- Dabrowska T, Sobala W. Pesticide exposure and birthweight: an epdemiological study in central Poland. Int J Occup Med Environ Health 2003; 16: 31- 40
• Srivastava MK, Raizada RB. Development effect of technical dimethoate in rats: maternal and fetal toxicity evaluation. Indian J Exp Biol 1996; 34: 329- 33
• Farag TF, Karkour TA, Okazy AE. Developmental toxicity of orally administered technical dimethoate in rats. Birth Def Res (part B) 2006; 77: 40- 6
• Breslin WJ, Liberacki AB, Dittenber DA, Quast JF. Evaluation of the developmental and reproductive toxicity of chlorpyrifos in the rat. Fundam Appl Toxicol 1996; (1): 119- 30
• Tian Y, Ishikawa H, Yamauchi T, Yokoyama K. Teratogenicity and development toxicity of chlorpyrifos maternal exposure during organogenesis in mice. Repro Toxicol 2005; 20: 267- 71
• Karaöz E, Gültekin F, Akdoðan M, Öncü M, Gökçimen A. Protective role of melatonin and a combination of vitamin C and vitamin E on lung toxicity indued by chlorpyrifos-ethyl in rats. Exp Toxicol Pathol. 2002; 54: 97- 108
• Ögetürk M, Kuþ Ý, Çolakoðlu N, Zararsýz Ý, Ýlhan N, Sarsýlmaz M. Caffeic acid phenethyl ester protects kidneys againts carbon tetrachloride toxicity in rats. J Ethnopharmacol 2005;97: 273- 80
• Öktem F, Özguner F, Sulak O, Olgar S, Aktürk O, Yýlmaz HR ve ark. Lithium- induced renal toxicity in rats: Protection by a novel antioxidant caffeic acid phenethyl ester. Mol Cell Biochem 2005; 277: 109- 15