DOES EXCESSIVE CONSUMPTION OF FLAXSEED (LINUM USITATISSIMUM L.) CAUSE A LIVER DAMAGE IN RAT MODELS?

Year 2021, Volume: 28 Issue: 2, 333 - 341, 15.06.2021

Meltem Özgöçmen , Şükriye Yeşilot

https://doi.org/10.17343/sdutfd.887724

Cited By: 1

Abstract

Natural health products and functional foods are
frequently consumed for the prevention and treatment
of many diseases. Flaxseed is one of the functional foods with anticancer, antiviral, antibacterial and antifungal
properties. In addition to its beneficial effects,
excessive consumption without considering the appropriate
dosage can cause toxic effects. In this study,
it was planned to investigate the effects of different
amounts of flaxseed consumption on rat liver.
Materials and Methods
Rats were randomly divided into four groups with 8
rats in each groups; control group and experimental
groups which given flaxseed for 7 days; 1.4, 2.8 and
5.6 g/kg/day, respectively. At the end of the experiment,
histological, immunohistochemical and biochemical
analyzes were performed on tissue and blood
samples.
Results
Serum AST, ALT and ALP values are significantly higher
in 5.6 g/kg of flaxseed compared to control group
(p values 0.003, 0.012 and 0.009, respectively). Albumin
values were significantly lower in experimental
groups compared to the control group (p<0.05). TOS
and OSI increased significantly (p<0.05), TAS decreased
(p>0.05) in all experimental groups compared to
control. In histopathological findings, there was significant
difference in three groups compared to control
group, especially in 5.6 g/kg of flaxseed group compared
to others. As a result of immunohistochemical
analyzes, staining intensity of the receptors was highest
in 5.6 g/kg of flaxseed group. The highest positive
staining was observed respectively in NOX4, iNOS,
TNF-α and IL-6.
Conclusion
These results show that excessive consumption of
flaxseed can cause oxidative stress and toxicity due
to inflammation in the liver and this study can helpful
other studies about flaxseed toxicity.

Keywords

Flaxseed toxicity, Liver Toxicity, Rat

Supporting Institution

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Project Number

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Thanks

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AŞIRI DOZ KETEN TOHUMU (LINUM USITATISSIMUM L.) TÜKETİMİ RATLARDA KARACİĞER HASARINA NEDEN OLUR MU?

Abstract

Doğal sağlık ürünleri ve fonksiyonel gıdalar, birçok
hastalığın önlenmesi ve tedavisi için tüketilmektedir.
Keten tohumu antikanser, antiviral, antibakteriyel ve
antifungal özelliklere sahip fonksiyonel gıdalardan biridir.
Yararlı etkilerinin yanı sıra bilinçsiz ve aşırı tüketilmesi
toksik etkilere neden olabilmektedir. Bu çalışmada,
farklı miktarlarda keten tohumu tüketiminin
sıçan karaciğeri üzerindeki etkilerinin araştırılması
planlanmıştır.
Gereç ve Yöntem
Sıçanlar; her grupta 8 adet olarak; kontrol grubu ve
7 gün süreyle sırasıyla; 1.4, 2.8 ve 5.6 g/kg/gün keten
tohumu verilen deney grupları şeklinde dört gruba
ayrılmıştır. Deney sonunda doku ve kan örneklerinde
histolojik, immünohistokimyasal ve biyokimyasal analizler
yapılmıştır.
Bulgular
Serum AST, ALT ve ALP değerleri 5,6 g/kg keten tohumu
verilen grupta kontrol grubuna göre anlamlı
olarak daha yüksek gözlendi (p değerleri 0.003,
0.012 ve 0.009). Albümin değerleri deney gruplarında
kontrol grubuna göre anlamlı olarak düşük gözlendi
(p<0.05). TOS ve OSI kontrole göre tüm deney gruplarında
anlamlı olarak artmış (p<0.05), TAS ise azalmıştı
(p>0.05). Kontrol grubu ile deney grupları arasında
histopatolojik bulgular arasında anlamlı farklar
bulundu. Özellikle 5.6 g/kg keten tohumu grubunda
tüm gruplara göre daha fazla histopatolojik bulgulara
rastlandı. İmmünohistokimyasal analizlerde, en fazla
boyama yoğunluğu 5,6 g/kg keten tohumu grubunda
gözlemlendi. En yüksek pozitif boyanmaya sırasıyla
NOX4, iNOS, TNF-α ve IL-6 boyanmalarında rastlandı.
Sonuç
Aşırı keten tohumu tüketiminin oksidatif stres ve toksisiteye
bağlı olarak karaciğerde iltihaplanmaya neden
olabileceği ve çalışmanın keten tohumu toksisitesi ile
ilgili diğer çalışmalara katkı sağlayacağı düşünülmektedir.

Keywords

Keten tohumu toksisitesi, Karaciğer toksisitesi, Sıçan

Project Number

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