INVESTIGATION OF PROTECTIVE EFFECTS OF DIFFERENT DOSES OF OMEGA 3 FATTY ACIDS IN EXPERIMENTAL RENAL ISCHEMIA REPERFUSION DAMAGE

Year 2021, Volume: 28 Issue: 2, 283 - 289, 15.06.2021

Ferhat Şirinyıldız , Gökhan Cesur

https://doi.org/10.17343/sdutfd.891576

Abstract

Objective
Ischemia reperfusion (IR) injury is caused by a sudden
and temporary impairment of blood flow to a particular
organ or tissue. Renal IR-induced kidney damage causes
high levels of permanent damage and mortality.
Dietary omega-3 fatty acids have anti-inflammatory and immune-modulating effects and are used as protective
agents.
Materials and Methods
In the study; Protective effects of different doses of
omega-3 fatty acid in ischemia reperfusion injury were
investigated by biochemical and histological analyzes.
40 adult female rats were divided into 5 groups
as sham control, IR, IR+100, IR+400, IR+700 (n:8).
The right kidney was removed by nephrectomy in the
sham control group, and the left kidney was not treated.
For IR injury; the right kidney was removed by
nephrectomy, and the left kidney was clamped. Omega-
3 fatty acids of 100 mg/kg, 400 mg/kg and 700 mg/
kg were administered by gastric gavage to the treatment
groups for 14 days before the surgery and for
15. gastric gavage was administrated in the morning
of the operation.
Results
According these data; with application of omega-3,
histopathological examination results were correlated.
It is found that, histopathological results were
supported by biochemical results. There was a significant
decrease in malondialdehyde (MDA) and myeloperoxidase
(MPO) levels in the treated groups, while
catalase (CAT) and glutathione peroxidase (GSH-Px)
levels were detected to be increased.
Conclusion
It was concluded that administration of omega-3 fatty
acids beforehand in cases that may cause ischemia
and reperfusion damage in the kidney can provide a
protective effect on this tissue.

Keywords

Inflammation, Ischemia-Reperfusion Injury, Kidney, Omega 3 fatty acids, Oxidative stress

Supporting Institution

Aydın Adnan Menderes University Scientific Research Projects Unit

Project Number

TPF-16032

Thanks

The project was supported by the Scientific Research Projects Unit of Aydın Adnan Menderes University with the number TPF-16032.

DENEYSEL BÖBREK İSKEMİ REPERFÜZYON HASARINDA FARKLI DOZLARDAKİ OMEGA 3 YAĞ ASİTLERİNİN KORUYUCU ETKİLERİNİN ARAŞTIRILMASI

Abstract

Amaç
İskemi reperfüzyon (IR) hasarı, belirli bir organa kan
akışının ani ve geçici olarak bozulmasından kaynaklanır.
Renal IR kaynaklı böbrek hasarı, yüksek düzeyde
kalıcı hasar ve mortaliteye neden olur. Diyet ile
alınan omega-3 yağ asitleri, antiinflamatuar ve immün
modüle edici etkilere sahiptir ve koruyucu olarak kullanılmaktadır.
Çalışmada; iskemi reperfüzyon hasarında
omega-3 yağ asidinin farklı dozlarının koruyucu
etkileri biyokimyasal ve histolojik analizler ile incelenmiştir.
Gereç ve Yöntem
40 adet erişkin dişi sıçan Düz kontrol, sham kontrol,
IR, IR+100, IR+400, IR+700 olmak üzere 5 gruba ayrıldı
(n:8). Sham grubunda sağ böbrek nefrektomi ile
alındı, sol böbreğe işlem uygulanmadı. I/R hasarına
maruz kalan sağ böbrek nefrektomi ile alındı, sol böbreklere
ise klemp takıldı. Tedavi gruplarına cerrahi girişimden
önceki 14 gün ve operasyon gününün sabahında
toplam 15 sefer 100 mg/kg, 400 mg/kg ve 700
mg/kg’lık omega-3 yağ asitleri gastrik gavaj ile verildi.
Bulgular
Elde edilen verilere göre; artan dozlardaki omega-3
ile histopatolojik inceleme sonuçları korale şekilde iyileşme
tespit edildi. Histopatolojik sonuçlar, biyokimyasal
sonuçlar ile desteklendi. Malondaildehit (MDA) ve
myeloperoksidaz (MPO) düzeylerinde tedavi uygulanan
gruplarda anlamlı düzeyde azalma saptanırken,
katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) düzeylerinde
artış saptandı.
Sonuç
Sonuç olarak; elde edilen bulgulara göre, böbrekte
iskemi ve reperfüzyon hasarı oluşturma ihtimali olan
vakalarda önceden omega-3 yağ asidi verilmesinin bu
dokuda koruyucu etki sağlayabileceği sonucuna ulaşılmıştır.

Keywords

Böbrek, İnflamasyon, İskemi-Reperfüzyon Hasarı, Oksidatif stres, Omega 3 yağ asitleri

Project Number

TPF-16032

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