SURFACE MODIFIED TITANIUM DIOXIDE/POLY (LACTIC ACID) NANOCOMPOSITE FILMS FOR TISSUE ENGINEERING

Year 2022, Volume: 29 Issue: 1, 111 - 120, 01.03.2022

Şükran Melda Eskitoros Toğay , Ulya Tokgoz

https://doi.org/10.17343/sdutfd.1016353

Cited By: 2

Abstract

Objective
This study aims to synthesize and characterize the
nanocomposite films incorporating unmodified and
modified nanoparticles within the poly(lactic acid)
matrix, and to investigate their usage as an alternative
scaffold for tissue engineering.
Materials and Methods
Titanium dioxide (TiO2) nanoparticles were firstly
grafted by L-lactic acid oligomer (LA-g-TiO2) and the
mixture of propionic acid/hexylamine (AA-g-TiO2),
respectively. Then the unmodified and modified
nanoparticles were incorporated within the poly(lactic
acid) matrix via the solvent casting method to produce
the PLA/TiO2, PLA/LA-g-TiO2, and PLA/AA-g-TiO2
nanocomposite films. The chemical, thermal and
mechanical structures of these synthesized films were
subsequently characterized.
Results
The attenuated total reflectance (ATR) results
demonstrated that the surface modification of the
nanoparticles was accomplished. The results of
differential scanning calorimeter (DSC) analysis
showed that the crystallization of the PLA was
partly increased by the incorporation of modified
nanoparticles. The results of thermogravimetric
analysis (TGA) showed that the addition of LA-g-TiO2
into the polymer matrix improved the thermal stability
of PLA/LA-g-TiO2 nanocomposite film more than the
addition of AA-g-TiO2 into the polymer matrix. The
first and second decomposition temperatures of the
nanocomposites containing LA-g-TiO2 were 348.3 oC
and 392 oC, respectively, which were 6% greater than
those of the neat PLA. The micrograph of atomic force
microscopy (AFM) of the nanocomposites indicated
that LA-g-TiO2 and AA-g-TiO2 were homogeneously
dispersed in polymer matrices. The results of dynamic
mechanical analysis (DMA) demonstrated that the
most efficient bonding and compatibility were obtained
in PLA/LA-g-TiO2 nanocomposite compared to the
other nanocomposites.
Conclusion
These grafted nanoparticles, LA-g-TiO2 and AA-g-
TiO2, enhanced the thermal and mechanical properties
of the nanocomposites owing to their uniform
distribution in the matrix and good interactions with the
polymeric matrix. Therefore, these nanocomposites
can be utilized as alternative scaffolds in bone tissue
engineering.

Keywords

Poly(lactic acid), surface modified titanium dioxide, grafted, nanocomposite, nanoparticles, tissue engineering

Supporting Institution

Gazi Üniversitesi

Project Number

BAP 06/2012-03

Thanks

The authors are immensely grateful to Prof. Dr. Nursel Dilsiz for her endless help.

DOKU MÜHENDİSLİĞİ İÇİN YÜZEYİ MODİFİYE EDİLMİŞ TİTANYUM DİOKSİT/POLİ (LAKTİK ASİT) NANOKOMPOZİT FİLMLER

Abstract

Amaç
Bu çalışmanın amacı, poli(laktik asit) matrisi içerisinde
modifiye edilmemiş ve modifiye edilmiş nanopartiküller
içeren nanokompozit filmleri sentezlemek, karakterize
etmek ve doku mühendisliğinde alternatif bir
yapı iskelesi olarak kullanımlarını araştırmaktır.
Gereç ve Yöntem
İlk olarak, titanyum dioksit (TiO2) nanopartikülleri sırasıyla
L-laktik asit oligomeri (LA-g-TiO2) ve propiyonik
asit/heksilamin (AA-g-TiO2) karışımı ile aşılanmıştır.
Daha sonra, PLA/TiO2, PLA/LA-g-TiO2 ve PLA/AAg-
TiO2 nanokompozit filmleri üretmek için modifiye
edilmemiş ve modifiye edilmiş nanopartiküller solvent
döküm yöntemi ile poli (laktik asit) matrisi içine eklenmiştir.
Sentezlenen bu filmlerin kimyasal, termal ve
mekanik yapıları daha sonra karakterize edilmiştir.
Bulgular
Azaltılmış toplam yansıma (ATR) sonuçları, nanopartiküllerin
yüzey modifikasyonunun başarılı olduğunu
göstermiştir. Diferansiyel tarama kalorimetresi (DSC)
analizinin sonuçları, modifiye edilmiş nanopartiküllerin
dahil edilmesiyle PLA’nın kristalleşmesinin kısmen
arttığını göstermiştir. Termogravimetrik analizin
(TGA) sonuçları, polimer matrisine LA-g-TiO2 eklenmesinin,
PLA/LA-g-TiO2 nanokompozit filmin termal
stabilitesini, polimer matrisine AA-g-TiO2 ilavesinden
daha fazla geliştirdiğini göstermiştir. LA-g-TiO2 içeren
nanokompozitlerin birinci ve ikinci bozunma sıcaklıkları
sırasıyla 348.3 oC ve 392 oC, saf PLA’nınkinden
%6 daha yüksektir. Nanokompozitlerin atomik kuvvet
mikroskobu (AFM) mikrografı, LA-g-TiO2 ve AAg-
TiO2 nanopartiküllerin polimer matrislerde homojen
olarak dağıldığını göstermiştir. Dinamik mekanik analiz
(DMA) sonuçları, diğer nanokompozitlere kıyasla
PLA/LA-g-TiO2 nanokompozitinde en verimli bağlanma
ve uyumluluğun elde edildiğini göstermiştir.
Sonuç
Aşılanmış nanopartiküller, LA-g-TiO2 ve AA-g-TiO2,
matris içindeki homojen dağılımları ve polimerik matris
ile iyi etkileşimleri sayesinde nanokompozitlerin
termal ve mekanik özelliklerini iyileştirmiştir. Bu nedenle,
bu nanokompozitler kemik doku mühendisliğinde
alternatif doku iskeleleri olarak kullanılabilir

Keywords

Poli(laktik asit), yüzeyi modifiye edilmiş titanium dioksit, aşılama, nanokomposit, nanopartikül, doku mühendisliği

Project Number

BAP 06/2012-03

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