PLURİPOTENT KÖK HÜCRELERDEN SİNİR HÜCRELERİNE FARKLILAŞTIRMA YÖNTEMLERİ

Year 2022, Volume: 29 Issue: 4, 691 - 696, 27.12.2022

Meltem Kuruş Kemal Ergin Rahmi Çetinkaya

https://doi.org/10.17343/sdutfd.1103596

Abstract

İnsan embriyonik kök hücreleri, embriyoların erken
blastokist evresindeki iç hücre kütlesinden türetilen
hücrelerdir. Pluripotent özellikte olan bu hücreler, uygun
koşullar altında fonksiyonel nöronlara ve farklı
tipte sinir hücrelerine farklılaştırılabilmektedir. Ancak
bu alandaki en büyük zorluklardan biri, yenilenebilir,
kültürü kolay, nöral soylara bağlı nöral prekürsör
hücre popülasyonu oluşturmaktır. Bu nedenle, insan
embriyonik kök hücrelerini prekürsör hücrelere en
uygun şekilde farklılaştırmak, bunların kendi kendini
yenileyen bir popülasyon olarak devam etmesi ve
farklı bölgelerdeki sinir hücre tiplerini saf bir popülasyon
şeklinde üretmek için kritik öneme sahiptir. Hücre
sinyalleri ve bunlarla ilişkili moleküller de bu olaylarda
önemli bir rol oynamaktadır. Nöral prekürsör hücrelerinin
üretilmesi için kök hücre biyolojisinin ve nöral
hücrelere farklılaşmada rol oynayan önemli yolakların
daha iyi anlaşılması gerekmektedir. Bu derlemede
kök hücrelerden nöral hücrelere farklılaştırma yöntemlerine
ve bu süreçte önemli olan sinyal yolaklarına
ve moleküllere odaklanılmaktadır.

Keywords

Nöronal farklılaşma, Pluripotent kök hücre, Sinyal yolakları

Supporting Institution

İzmir Katip Çelebi Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

2016-ÖNP-TIPF-0028

Thanks

İzmir Katip Çelebi Üniversitesi BAP birimi 2016-ÖNP-TIPF-0028 numaralı proje çerçevesince desteklerinden dolayı teşekkür ederiz.

METHODS OF DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO NEURAL CELLS

Abstract

Human embryonic stem cells are derived from inner
cell mass of early stage blastocyst embryos. These
cells are pluripotent and can differentiate into functional
neurons and various nerve cells under appropriate
conditions. However, one of the biggest challenges in
this area is to establish a renewable, easy-to-culture,
neural lineage-linked neural precursor cell population.
Therefore, it is crucial to conveniently differentiate
human embryonic stem cells into precursor cells,
maintain them as a self-renewing population, and
achieve nerve cell types from different regions as a
pure population. Cell signals and their associated
molecules also play an important role in these events.
For the generation of neural precursor cells, a better
understanding of stem cell biology and the important
pathways involved in differentiation into neural cells
is required. This review focuses on the differentiation
methods from stem cells to neural cells and the
important signaling pathways and molecules in this
process.

Keywords

Neuronal Differentiation, Pluripotent Stem Cell, Signaling pathways, Nöronal farklılaşma, Pluripotent kök hücre, Sinyal yolakları

Project Number

2016-ÖNP-TIPF-0028

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