The Protective Effects of Cannabidiol on Chest Trauma-Induced Brain Injury by its Antioxidant and Anti-Inflammatory Effects

Year 2024, Volume: 31 Issue: 4, 332 - 338, 26.12.2024

Adem Milletsever , Abdurrahman Gülal , Halil Aşçı

https://doi.org/10.17343/sdutfd.1514644

Abstract

Objective: Chest trauma-induced brain injury (CTBI) is caused by the formation of inflammatory cytokines in the lungs and blood. Cannabidiol (CBD), a non-psychoactive agent, has antioxidant, anti-inflammatory, and antiapoptotic properties. In this study, we aimed to investigate the protective effects of CBD on CTBI.
Material and Method: Forty male Wistar Albino rats were divided into four groups: control, CTBI (200 g weight drop on the anterior chest wall from a height of 1 meter), CTBI+CBD (5 mg/kg, single dose intraperitoneally), and CBD. After 48 hours, rats were sacrificed under anesthesia, and brain tissues were placed in a 10% formaldehyde solution for histopathological and immunohistochemical examination.
Results: In the CTBI group, hemorrhagic areas, tumor necrosis factor-alpha, caspase-3, and malondialdehyde expressions increased in histological and immunohistochemical examinations compared to the control group. CBD treatment reduced hemorrhagic areas and reversed immune expressions.
Conclusion: Inflammation, apoptosis, and oxidative stress in brain tissue may develop in CTBI. These damages can be corrected with CBD treatment.

Keywords

Blunt chest trauma, brain, cannabidiol, apoptosis, malondialdehyde

Ethical Statement

The study was carried out at Süleyman Demirel University Experimental Animal Production and Experimental Research Laboratory. Ethical approval was obtained from the National Institutes of Health and approved by the Local Ethics Committee for Animal Experiments of Suleyman Demirel University (Approval No. 2023-01/116).

Supporting Institution

This study was supported by the project coded "TSG-2023-9010," funded by the Scientific Research Coordination Unit of Süleyman Demirel University.

Project Number

TSG-2023-9010

Thanks

None.

References

• 1. Grubmüller M, Kerschbaum M, Diepold E, Angerpointner K, Nerlich M, Ernstberger A. Severe thoracic trauma–still an independent predictor for death in multiple injured patients? Scandinavian Journal Of Trauma, Resuscitation and Emergency Medicine 2018;26:1-8.
• 2. Cassuto J, Ezuddin N, Danton G. Blunt chest trauma: A radiologic approach and review. Current Radiology Reports 2018;6:1-11.
• 3. Rachfalska N, Putowski Z, Krzych ŁJ. Distant organ damage in acute brain injury. Brain Sciences 2020;10(12):1019.
• 4. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4‐hydroxy‐2‐nonenal. Oxidative Medicine And Cellular Longevity 2014;2014(1):360438.
• 5. Calapai F, Cardia L, Sorbara EE, Navarra M, Gangemi S, Calapai G, Mannucci C. Cannabinoids, blood–brain barrier, and brain disposition. Pharmaceutics 2020;12(3):265.
• 6. Atalay S, Jarocka-Karpowicz I, Skrzydlewska E. Antioxidative and anti-inflammatory properties of cannabidiol. Antioxidants 2019;9(1):21.
• 7. Raghavendran K, Davidson BA, Helinski JD, Marschke CJ, Manderscheid P, Woytash JA, et al. A rat model for isolated bilateral lung contusion from blunt chest trauma. Anesthesia & Analgesia 2005;101(5):1482-9.
• 8. Fouad AA, Albuali WH, Al-Mulhim AS, Jresat I. Cardioprotective effect of cannabidiol in rats exposed to doxorubicin toxicity. Environmental Toxicology and Pharmacology 2013;36(2):347-57.
• 9. Mielke D, Bleuel K, Stadelmann C, Rohde V, Malinova V. The ESAS-score: A histological severity grading system of subarachnoid hemorrhage using the modified double hemorrhage model in rats. PloS One 2020;15(2):e0227349.
• 10. Eltzschig HK, Carmeliet P. Hypoxia and inflammation. New England Journal of Medicine 2011;364(7):656-65.
• 11. Yang J, Ran M, Li H, Lin Y, Ma K, Yang Y, et al. New insight into neurological degeneration: Inflammatory cytokines and blood–brain barrier. Frontiers in Molecular Neuroscience 2022;15:1013933.
• 12. Miller MA, Zachary JF. Mechanisms and morphology of cellular injury, adaptation, and death. Pathologic Basis of Veterinary Disease 2017:2.