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Development of Hybrid Breeding Lines by Immature Seeds Culture Containing Zygotic Embryos of Safflower (Carthamus tinctorius L.) Plant

Year 2018, Volume: 13 Issue: 2, 79 - 87, 31.12.2018

Abstract

In this study, the shortening of the breeding period was targeted in safflower (Carthamus tinctorius L.) by zygotic immature seeds culture on the 12th day after hybridization. Dinçer 5-118, Remzibey-05 and Balcı varieties were used in this study and the hybridizations were done according to half-diallel hybridization method. The general success rate was 48.55% in hybridization and 584 numbers zygotic immature seeds were cultured from 150 receptacle during the study. As a result, the highest success rate of regeneration in cultured embryos was obtained from M5519 medium (15.82%) followed by MSD4 (13.43%) and LS2.5 mediums (6.21%). As a result, hybrid seed production can be achieved by using the embryo or zygotic immature seeds culture technique in the safflower and the breeding process can be shortened. Based on these results, the nutrienth media, used for embryo culture, should not be including hormones such as BAP and 2.4-D. If the growth regulators are added after seed germination and the plant is obtained, will be increase success rate.

References

  • Culpan, E. ve Arslan, B. 2017. Aspir Bitkisinde (Carthamus tinctorius L.) Islah Çalışmaları. International Congress of the New Approaches and Technologies for Sustainable Development September 21-24, 2017 Isparta / TURKEY https://www.researchgate.net/profile/Emrullah_Culpan/publication/320449850_Breeding_Studies_on_Safflower_Carthamus_tinctorius_L/links/59e5f92ea6fdcc1b1d97022f/Breeding-Studies-on-Safflower-Carthamus-tinctorius-L.pdf
  • Dajue L. and H. H. Mündel 1996. Safflower, promoting the conservation and use of underutilized and neglected crops. 7. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, Italy (ISBN92-9043-297-7). 85 pp. https://www.bioversityinternational.org/fileadmin/_migrated/uploads/tx_news/Safflower__Carthamus_tinctorius_L._498.pdf
  • FAO 2008. http://www.fao.org/faostat/en/#data/QC . Erişim Tarihi: 07.04.2018.
  • Golkar, P. 2014. Breeding improvements in safflower (Carthamus tinctorius L.): A review. Australian Journal of Crop Science, 8(7):1079-1085. http://www.cropj.com/golkar_8_7_2014_1079_1085.pdf
  • Kayaçetin, F., Katar, D. ve Arslan, Y. 2012. Aspir (Carthamus tinctorius L.)’in Döllenme Biyolojisi ve Çiçek Yapısı. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 21 (2): 75-80. http://dergipark.gov.tr/download/article-file/118431
  • Malik, S.I., Rashid, H., Yasmin, T. and Minhas, N.M. 2003. Effect of 2,4- dichlorophenoxyacetic Acid on Callus Induction from Mature Wheat (Triticum aestivum L.) Seeds. International Journal of Agriculture & Biology. 1560–8530/06–1–156–159. https://www.researchgate.net/publication/242453014_Effect_of_24-dichlorophenoxyacetic_Acid_on_Callus_Induction_from_Mature_Wheat_Triticum_aestivum_L_Seeds
  • Öztürk Ö., Akınerdem, F., Bayraktar, N. ve Ada, R. 2007. Konya koşullarında bazı aspir çeşitlerinin verim, verim unsurları ve yağ oranlarının incelenmesi. I. Ulusal Yağlı Tohumlu Bitkiler ve Biyodizel Sempozyumu, 191-202, 28-31 Mayıs, Samsun.
  • Singh, R.J. 2007. Genetic resources, chromosome engineering and crop improvement. CRC Press Inc. Boca Raton, Florida, USA.Uysal, N., Baydar, H. ve Erbaş S. 2006. Isparta Populasyonundan Geliştirilen Aspir (Carthamus tinctorius L.) Hatlarının Tarımsal ve Teknolojik Özelliklerinin Belirlenmesi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi 1(1):52-63. edergi.sdu.edu.tr/index.php/zfd/article/download/204/103
  • Zheng, M.Y. and Konzak, C.F. 1999. Effect of 2,4-dichlorophenoxyacetic acid on callus induction and plant regeneration in anther culture of wheat (Triticum aestivum L.). Plant Cell Reports, November 1999, Volume 19, Issue 1, pp 69–73. https://link.springer.com/article/10.1007/s002990050712

Aspir (Carthamus tinctorius L.) Bitkisinde Zigotik Embriyoları İçeren Tohum Taslaklarının Kültürü ile Melez Islah Hatlarının Geliştirilmesi

Year 2018, Volume: 13 Issue: 2, 79 - 87, 31.12.2018

Abstract

Bu
araştırmada Aspir (Carthamus tinctorius
L.) bitkisinde melezlemeden sonraki 12. günde zigotik embriyoları içeren tohum
taslaklarının kültürü ile Aspirde ıslah sürecinin kısaltılması hedeflenmiştir.
Araştırmada Dinçer, Remzibey-05 ve Balcı Aspir çeşitleri kullanılmış olup, yarı
diallel melezleme yöntemine göre melezleme yapılmıştır. Melezlemede genel
başarı oranı %48,55 oranında gerçekleşmiş olup, araştırma süresince toplam 150
tabladan 584 adet tohum taslağı kültüre alınmıştır.



Sonuç
olarak kültüre alınan embriyolarda meydana gelen rejenerasyon bakımından en
yüksek başarı oranı M5519 besi ortamından elde edilirken (%15,82), bunu MSD4
(%13,43), ve LS2,5 besi ortamları (%6,21) takip etmiştir.

References

  • Culpan, E. ve Arslan, B. 2017. Aspir Bitkisinde (Carthamus tinctorius L.) Islah Çalışmaları. International Congress of the New Approaches and Technologies for Sustainable Development September 21-24, 2017 Isparta / TURKEY https://www.researchgate.net/profile/Emrullah_Culpan/publication/320449850_Breeding_Studies_on_Safflower_Carthamus_tinctorius_L/links/59e5f92ea6fdcc1b1d97022f/Breeding-Studies-on-Safflower-Carthamus-tinctorius-L.pdf
  • Dajue L. and H. H. Mündel 1996. Safflower, promoting the conservation and use of underutilized and neglected crops. 7. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, Italy (ISBN92-9043-297-7). 85 pp. https://www.bioversityinternational.org/fileadmin/_migrated/uploads/tx_news/Safflower__Carthamus_tinctorius_L._498.pdf
  • FAO 2008. http://www.fao.org/faostat/en/#data/QC . Erişim Tarihi: 07.04.2018.
  • Golkar, P. 2014. Breeding improvements in safflower (Carthamus tinctorius L.): A review. Australian Journal of Crop Science, 8(7):1079-1085. http://www.cropj.com/golkar_8_7_2014_1079_1085.pdf
  • Kayaçetin, F., Katar, D. ve Arslan, Y. 2012. Aspir (Carthamus tinctorius L.)’in Döllenme Biyolojisi ve Çiçek Yapısı. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 21 (2): 75-80. http://dergipark.gov.tr/download/article-file/118431
  • Malik, S.I., Rashid, H., Yasmin, T. and Minhas, N.M. 2003. Effect of 2,4- dichlorophenoxyacetic Acid on Callus Induction from Mature Wheat (Triticum aestivum L.) Seeds. International Journal of Agriculture & Biology. 1560–8530/06–1–156–159. https://www.researchgate.net/publication/242453014_Effect_of_24-dichlorophenoxyacetic_Acid_on_Callus_Induction_from_Mature_Wheat_Triticum_aestivum_L_Seeds
  • Öztürk Ö., Akınerdem, F., Bayraktar, N. ve Ada, R. 2007. Konya koşullarında bazı aspir çeşitlerinin verim, verim unsurları ve yağ oranlarının incelenmesi. I. Ulusal Yağlı Tohumlu Bitkiler ve Biyodizel Sempozyumu, 191-202, 28-31 Mayıs, Samsun.
  • Singh, R.J. 2007. Genetic resources, chromosome engineering and crop improvement. CRC Press Inc. Boca Raton, Florida, USA.Uysal, N., Baydar, H. ve Erbaş S. 2006. Isparta Populasyonundan Geliştirilen Aspir (Carthamus tinctorius L.) Hatlarının Tarımsal ve Teknolojik Özelliklerinin Belirlenmesi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi 1(1):52-63. edergi.sdu.edu.tr/index.php/zfd/article/download/204/103
  • Zheng, M.Y. and Konzak, C.F. 1999. Effect of 2,4-dichlorophenoxyacetic acid on callus induction and plant regeneration in anther culture of wheat (Triticum aestivum L.). Plant Cell Reports, November 1999, Volume 19, Issue 1, pp 69–73. https://link.springer.com/article/10.1007/s002990050712
There are 9 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering
Journal Section Research
Authors

Hüseyin Uysal 0000-0003-4187-9149

Emine Aslan This is me

Özge Erden This is me

Publication Date December 31, 2018
Submission Date July 24, 2018
Acceptance Date December 28, 2018
Published in Issue Year 2018 Volume: 13 Issue: 2

Cite

APA Uysal, H., Aslan, E., & Erden, Ö. (2018). Aspir (Carthamus tinctorius L.) Bitkisinde Zigotik Embriyoları İçeren Tohum Taslaklarının Kültürü ile Melez Islah Hatlarının Geliştirilmesi. Ziraat Fakültesi Dergisi, 13(2), 79-87.