Investigation of the Usage Possibilities of Some Essential Oils Against Potato Pathogens Fusarium sambucinum and Rhizoctonia solani

Year 2024, Volume: 19 Issue: 2, 60 - 74, 31.12.2024

Şerife Evrim Arıcı , Arif Şanlı , Raziye Dilara Çapa

https://doi.org/10.54975/sduzfd.1565440

Abstract

The antifungal activity of four essential oils (Sage, Rosemary, Cinnamon and Thyme) against Fusarium sambucinum and Rhizoctonia solani causing rot in stored potatoes were investigated in the contact and vapor phase In contact effect experiments of each oil, 100, 200, 300, 400, 500, 1000, 2000, 4000 𝜇L/L, and in vapor phase experiments, 3, 6.25, 12.5, 25, 50, 100 𝜇L/petri doses were used. Although all oils inhibited the mycelial growth of fungi in in vitro studies, the most effective essential oils were cinnamon and thyme. In the contact phase application, 100 𝜇L/L dose of thyme oil inhibited the mycelial growth of both fungi by 100%, while cinnamon oil at the doses of 200 𝜇L/L for R. solani and 300 𝜇L/L for F. sambucinum inhibited the mycelial growth by 100%. In the vapor phase, 25 𝜇L/petri dose of cinnamon oil, 6.25 𝜇L/petri dose of thyme oil for R. solani and 12.5 𝜇L/petri dose of both oils for F. sambucinum inhibited the mycelial growth of fungi by 100. Thyme and cinnamon oil in the contact application on potato tubers, mycelial growth of both fungi was completely suppressed, while thyme oil showed better efficacy in the vapor phase. Thymol (44.63%) was determined as the main component in thyme oil and cinnamaldhyde (66.66%) was determined as the main component in cinnamon oil. In conclusion, it is thought that the contact phase of thyme and cinnamon oils can be used as alternative antimicrobials to fungicides in controlling F. sambucinum and R. solani on potato tubers.

Keywords

thymol, cinnamaldhyde, antifungal activity, essential oil

Project Number

2209/A Üniversite Öğrencileri Araştırma Projeleri Destek Programı kapsamında 1919B012201802 nolu

Patates Patojenleri Fusarium sambucinum ve Rhizoctonia solani’ye Karşı Bazı Uçucu Yağların Kullanım Olanaklarının Araştırılması

Abstract

Bu çalışmada patateslerde çürüklüklere neden olan Fusarium sambucinum ve Rhizoctonia solani’ye karşı 4 ayrı bitkiden (Adaçayı, Biberiye, Tarçın ve Kekik) elde edilen uçucu yağın kontakt ve buhar fazında antifungal aktivitesi araştırılmıştır. Her yağın kontakt etkili denemelerinde 100, 200, 300, 400, 500, 1000, 2000, 4000 𝜇L/L, buhar fazı denemelerinde ise 3, 6.25, 12.5, 25, 50, 100 𝜇L/petri dozları kullanılmıştır. In vitro çalışmalarında bütün yağlar fungusların misel gelişimini engellemesine rağmen, en etkili tarçın ve kekik uçucu yağları tespit edilmiştir. Kontakt faz uygulamasında kekik yağının 100 𝜇L/L dozu her iki fungusun misel gelişimini %100 engellerken, tarçın yağı R. solani için 200 𝜇L/L, F. sambucinum için 300 𝜇L/L dozlarında misel gelişimini %100 engellemiştir. Buhar fazında ise R. solani için tarçın yağı 25 𝜇L/petri, kekik yağı ise 6.25 𝜇L/petri dozu, F.sambucinum için her iki yağın 12.5 𝜇L/petri dozu fungusların misel gelişimlerini %100 engellemiştir. Patates yumrularında kekik ve tarçın yağının kontakt olarak uygulanmasında her iki fungusun misel gelişimi tamamen baskılanırken, buhar fazında kekik yağı daha iyi etkinlik göstermiştir. Kekik yağında thymol (%44.63), tarçın yağında ise cinnamaldhyde (%66. 66) ana bileşen olarak belirlenmiştir. Sonuç olarak kekik ve tarçın yağlarının kontakt fazı patates yumrularında F. sambucinum ve R. solani’yi kontrol etmede fungisitlere alternatif antimikrobiyal olarak kullanılabileceği tespit edilmiştir.

Keywords

antifungal aktivite, uçucu yağlar, thymol, cinnamaldhyde

Ethical Statement

Makale yazarları olarak herhangi bir etik kurul onay bilgileri beyanımız bulunmadığını bildiririz.

Supporting Institution

TUBİTAK

Project Number

2209/A Üniversite Öğrencileri Araştırma Projeleri Destek Programı kapsamında 1919B012201802 nolu

Thanks

2209/A Üniversite Öğrencileri Araştırma Projeleri Destek Programı kapsamında 1919B012201802 nolu çalışmanın gerçekleşmesinde maddi destek sağlayan TÜBİTAK’a teşekkür ederiz.

References

• Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology ,18, 265–267
• Adams, P. R. (2007). Identification of essential oil components by gas chromatography/ mass spectrometry, 4th Edition. Allured Publ., Carol Stream, IL, USA
• Agrios, G. N. (2015). Plant Pathology, 6th Edition. Academic Press, New York.
• Ahmad, I., Soomro, M. H., Khalid, S., Iftikhar, S., Munir, A., & Burney, K. (1995). Recent distributional trends of potato diseases in pakistan. national seminar on research and development of potato production in pakistan, April 23-25, NARC, PSPDP, PARC, Islamabad, Pakistan.
• Aksit, H., Bayar, Y., Simsek, S., & Ulutas, Y. (2022). Chemical composition and antifungal activities of the essential oils of thymus species (Thymus pectinatus, Thymus convolutus, Thymus vulgaris) against plant pathogens. Journal of Essential Oil Bearing Plants, 25(1), 200–207. https://doi.org/10.1080/0972060X.2022.2043189
• Al-Baldawy, M. S. M., Matloob, A. A., & Almammory, M. K. (2021). Effect of plant extracts and biological control agents on Rhizoctonia solani Kuhn. In IOP Conference Series: Earth and Environmental Science, 735(1), 012079.
• Al-Mughrabi, K. I., Coleman, W. K., Vikram, A., Poirier, R., & Jayasurija, K. E. (2013). Effectiveness of essential oils and their combinations with aluminium starch octenylsuccinate on potato storage pathogens. Journal of Essential Oil Bearing Plants, 16, 23–31. https://doi.org/10.1080/0972060X.2013.764201
• Al-Mughrabi, K. I. (2003). Antimicrobial activity of extracts from leaves, stems and flowers of Euphorbia macroclada against plant pathogenic fungi. Phytopathologia Mediterranea, 42, 245-250.
• Arıcı, S. E., & Sanlı, A. (2014). Effect of some essential oils against Rhizoctonia solani and Streptomycetes scabies on potato plants in field conditions. Annual Research & Review in Biology, 4(12), 2027.
• Aydın, M.H., & Turhan, G. (2013).The efficacy of Trichoderma species against Rhizoctonia solani in potato and their integration with some fungicides. Anadolu Journal of Aegean Agricultural Research Institute, 23(1), 12-30.
• Aydın, H., & Altın, N. (2022). Biberiye bitkisine ait uçucu yağın in vitro koşullarda Alternaria brassicicola etmenine karşı etkilerinin belirlenmesi. Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi, 2(1), 1-8.
• Baydar, H., Altındal D., & Karadogan T. (2009). Effects of volatile oils on sprout growth in potato. Suleyman Demirel Universitesi Fen Bilimleri Enstitusu Dergisi, 13, 137-141.
• Bisht, D., Saroj, A., Durgapal, A., Chanotiya, C. S., & Samad, A. (2021). Inhibitory effect of cinnamon (Cinnamomum tamala (Buch.-Ham.) T. Nees & Eberm.) essential oil and its aldehyde constituents on growth and spore germination of phytopathogenic fungi. Trends in Phytochemical Research, 5(2), 62-70.
• Boyd, A.E.W. (1972). Potato storage diseases. Review of Plant Pathology, 51, 297-321
• Bounar, R., Krimat, S., Boureghda, H., & Dob, T. (2020). Chemical analyses, antioxidant and antifungal effects of oregano and thyme essential oils alone or in combination against selected Fusarium species. International Food Research Journal, 27(1), 66-77.
• Bozhüyük, A. U., Kordali, Ş., & Bölük, G. (2015). Satureja hortensis L. uçucu yağının antifungal etkisi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 46(2), 107-112.
• Camele, I., Altieri, L., De Martino, L., De Feo, V., Mancini, E., & Rana, G. L. (2012). In vitro control of post-harvest fruit rot fungi by some plant essential oil components. International Journal Of Molecular Sciences, 13(2), 2290-2300. https://doi.org/10.3390/ijms13022290
• Chen, Q., Yuan, L., Guan, Z., Yue, F., Han, C., & Fu, M. (2024). Screening of plant essential oils as potato sprout suppressants at low storage temperature. Potato Research, 67(1), 325-338. https://doi.org/10.1007/s11540-023-09637-5
• Ćosić, J., Vrandečić, K., Poštić, J., Jurković, D., & Ravlić, M. (2010). In vitro antifungal activity of essential oils on growth of phytopathogenic fungi. Poljoprivreda, 16(2), 25-28.
• Delen, S. (2007). Bazı Fusarium türlerinin teşhisini kolaylaştırmaya yönelik bilgisayar programı. Yüksek Lisans Tezi, Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Konya,149s
• El-Awady, A. A. E. F. (2020). Effect of essential oils on storability and preservation of some vegetable Crops, In Book: El-Shemy, H (ed.). Essential oils - Oils of Nature, IntechOpen, London. 10.5772/intechopen.77673
• Gakuubi, M. M., Maina, A.W., & Wagacha, J.M. (2017). Antifungal activity of essential oil of Eucalyptus camaldulensis Dehnh. against Selected Fusarium sambucinumi. International Journal of Microbiology, 8761610. https://doi.org/10.1155/2017/8761610
• Ghoneem, K. M., Saber, W. I. A., El-Awady, A. A., Rashad, Y. M., & Al-Askar, A. A. (2016). Alternative preservation method against Sclerotium tuber rot of Jerusalem artichoke using natural essential oils, Phytoparasitica, 44, 341–352. https://doi.org/10.1007/s12600-016-0532-3
• Gómez-Castillo, D., Cruz, E., Iguaz, A., Arroqui, C., & Vírseda, P. (2013). Effects of essential oils on sprout suppression and quality of potato cultivars. Postharvest Biology And Technology, 82, 15-21. https://doi.org/10.1016/j.postharvbio.2013.02.017
• Hanson, L. E., Schwager, S. J., & Loria, R., (1996). Sensitivity to thiabendazole in Fusarium species associated with dry rot of potato. Phytopathology, 86, 378-384
• Hassan, S., Singh, R., Yadav, A. K., & Kumar, A. (2022). Antifungal effect of some essential oils and seed dressing fungicides against Fusarium sambucinum causing dry rot of potato. Plant Disease Research, 37(1), 40-45.
• Jadoa, H. N., & Ibade, K. W. (2023). Efficiency of rosemary Rosmarinus officinalis and chitosan in controlling the damping off pathogen, fusarium spp., ıdentified morphologically and molecularly. In IOP Conference Series: Earth and Environmental Science, 1262, (3), 032017.
• Kowalska, J., Tyburski, J., Matysiak, K., Jakubowska, M., Łukaszyk, J., & Krzymińska, J. (2021). Cinnamon as a useful preventive substance for the care of human and plant health. Molecules, 26(17), 5299. https://doi.org/10.3390/molecules26175299
• Little, G., Marquinez, R., & Cooke, L.R., (1988). The response of twelve potato cultivars to infection with Rhizoctonia solani. Tests of Agrochemicals and Culivars 9. Annals of Applied Biology, 112, 88-89.
• Massoud, M. A., Saad, A. S. A., Soliman, E. A., & El-Moghazy, A. Y. (2012). Antifungal activity of some essential oils applied as fumigants against two stored grains fungi. Journal of the Advances in Agricultural Researches, 17(2), 296-306. https://doi.org/10.3390/molecules26175299
• Morkeliūnė, A., Rasiukevičiūtė, N., Šernaitė, L., & Valiuškaitė, A. (2021). The use of essential oils from thyme, sage and peppermint against Colletotrichum acutatum. Plants, 10(1), 114. https://doi.org/10.3390/plants10010114
• Mosciano, G. (2005). Organoleptic characteristics of flavor materials: grapefruit, lemon, lime, clementine oil and beyond. Perfumer & flavorist, 30( 1),76-81
• Nohutçu, L., Şelem, E., Tunçtürk, R., & Tunçtürk, M. (2021). Yayın Yılı. Uçucu yağların tarımsal hastalık ve zararlılara karşı kullanımı. Bursa Uludağ Üniversitesi Ziraat Fakültesi Dergisi, 35(2), 499-523
• Oktay, E., & Artık, N. (2021). Nane Yağı ve Karanfil Yağı Uygulamasının Depolanan Patateslerde Filizlenmeye Etkisi. Avrupa Bilim ve Teknoloji Dergisi, (22), 357-363.
• Osman Mohamed Ali E., Shakil, N.A., Rana, V.S., Sarkar, D.J., Majumder, S., & Kaushik, P. (2017). Antifungal activity of nano emulsions of neem and citronella oils against phytopathogenic fungi, Rhizoctonia solani and Sclerotium rolfsii. Industrial Crops and Products, 10, 379– 387. https://doi.org/10.1016/j.indcrop.2017.06.061
• Paramanik, P. K., & Mandal, N. C. (2016). Dry rot of potato (co Fusarium oxysporum Schlecht) and its management by essential oils. Journal of Mycopathological Research, 54(2), 225-228
• Rangsuwan S., Rattanakreetakul C., & Pongpisutta R. (2021). Competency of clove and cinnamon essential oil fumigation against toxigenic and atoxigenic Aspergillus flavus Isolates. Journal of Pure and Applied Microbiology, 15(3),1325-1337. https://doi.org/10.22207/JPAM.15.3.23
• Pina-Vaz, C., Gonçalves Rodrigues, A., Pinto, E., Costa-de-Oliveira, S., Tavares, C., Salgueiro, L., Cavaleiro, C., Gonçalves, MJ., & Martinez-de-Oliveira, J. (2004): Antifungal activity of Thymus oils and their major compounds. Journal of the European Academy of Dermatology and Venereology 18, 73–78.
• Read, P.J., Hide, G .A., Firmager, J.P., & S.M. Hall. (1989). Growth and yield of potatoes as affected by severity of stem canker (Rhizoctonia solani). Potato Research, 32, 9-15.
• Rostad, C.E., & Pereira, W.E. (1986). Kovats and Lee retention indices determined by gas chromatography/mass spectrometry for organic compounds of environmental interest. Journal of High Resolution Chromatography, 9, 328-334. https://doi.org/10.1002/jhrc.1240090603.
• Sanlı, A., & Karadogan, T. (2019). Carvone containing essential oils as sprout suppressants in potato (Solanum tuberosum L.) tubers at different storage temperatures. Potato Research, 3, 345–360. https://doi.org/10.1007/s11540-019-9415-6
• Saroj, A., Chanotiya, C. S., Maurya, R., Pragadheesh, V. S., Yadav, A., & Samad, A. (2019). Antifungal action of Lippia alba essential oil in Rhizoctonia solani disease management. SN Applied Sciences, 1, 1-12. https://doi.org/10.1007/s42452-019-1207-8
• Secor, G.A., & Salas, B. (2001). Fusarium dry rot and Fusarium wilt. In: Stevenson WR, Loria R, Franc GD, Weingartner DP (editors). Compendium of Potato Diseases. St. Paul, MN, USA: APS Press, pp. 23-25
• Sivrikaya, I. S., Tosun, B., & Karakaya, E. (2021). Origanum onites L. ve Rosmarinus officinalis L. uçucu yağlarının kimyasal içerikleri ve Fusarium solani’ye karşı antifungal aktivitelerinin belirlenmesi. Türk Tarım ve Doğa Bilimleri Dergisi, 8(2), 329-335. https://doi.org/10.30910/turkjans.825090
• Townsend, G.K., & Heuberger, J.W. (1943). Methods for estimating losses caused by diseases in fungicide experiments. Plant Disease Report, 27: 340-343.
• TÜİK, (2024). https://data.tuik.gov.tr/Bulten/Index?p=Bitkisel-Uretim-Istatistikleri-2023-49535 (Erişim Tarihi: 28.09.2024)
• Türkkan, M., Çalışkan, Ö., Erper, İ., Kara, Ş. M., & Açıkgöz, M. A. (2020). Bazı toprak kökenli funguslara karşı defne esansiyel yağı ve hidrosölünün antifungal etkilerinin belirlenmesi. Akademik Ziraat Dergisi, 8(2), 217-226. https://doi.org/10.29278/azd.476209
• Wharton, P., Hammerschmidt, R., & Kirk, W. (2007). Fusarium dry rot. Michigan State University. Extension Bulletin E-2992. East Lansing, Michigan.
• Woodhall, J.W., Lees, A.K., Edwards, S.G., & Jenkinson P. (2007). Characterization of Rhizoctonia solani from potato in Great Britain. Plant Pathology, 56, 286–295. https://doi.org/10.1111/j.1365-3059.2006.01545.x
• Yeole, G. J., Teli, N. P., Kotkar, H. M., & Mendki, P. S. (2014). Cinnamomum zeylanicum extracts and their formulations control early blight of tomato. Journal of Biopesticides, 7(2), 110.
• Yıkılmazsoy, G., & Tosun, N. (2021). Characterization of Fusarium sambucinum isolates associated with potato dry rot and evaluation of cultivar susceptibility and fungicides. Turkish Journal of Agriculture and Forestry, 45(2), 222-233. https://doi.org/10.3906/tar-2006-100
• Yilar, M., Kadioglu, I., & Telci, I. (2018). Chemical composition and antifungal activity of Salvia officinalis (L.), S. cryptantha (Montbret Et Aucher Ex Benth.), S. tomentosa (Mıll.) plant essential oils and extracts. Fresenıus Envıronmental Bulletın, 27(3), 1695-1706
• Yücel, D., & Oğuz, H. I. (2020). Nevşehir ilinde patates (Solanum tuberosum L.) yetiştiriciliğinin ekolojik ve sosyo ekonomik bakımdan araştırılması. Türk Tarım ve Doğa Bilimleri Dergisi, 7(4),1159-1170 https://doi.org/10.30910/turkjans.727903