Comparison of Bond Strength of Biomaterials to Universal Adhesive Systems: An In Vitro Study

Year 2022, , 513 - 519, 24.08.2022

Yasemin Yavuz

https://doi.org/10.15311/selcukdentj.1140867

Cited By: 2

Abstract

Aim
The aim of this study is to compare the shear bond strength of calcium silicate-containing biomaterials to Universal adhesive systems with different pH.
Materials and Methods
63 acrylic blocks with cylindrical cavities (4 mm diameter - 2 mm depth) were prepared in square molds with a diameter of 20 millimeters (mm) and a depth of 20 mm. It was divided into nine groups. In accordance with the instructions of the manufacturers, 21 pieces of each biomaterial (NeoPutty, Theracal PT, Biodentine) were placed in the prepared cavities. Universal Adhesive systems on biomaterials; After Gluma Bond Universal, Single Bond Universal, G-Premio Bond were applied according to the manufacturer's instructions, the composite resin was placed in cylindrical plastic tube with a diameter of 2 mm and a height of 2 mm prepared from polyethylene and polymerized with a LED light device for 20 seconds. Shear bond strength of all samples were measured in Newtons using a universal tester. According to the results of Shapiro-wilk test, One Way (One Way ANOVA) Analysis of Variance method, statistical parametric tests, was applied. The groups were compared among themselves with the Post hoc Tukey HSD test.
Results
The difference between Biodentin and TheraCal PT groups was statistically significant P<0.05. The difference between Theracal PT and NeoPutty groups was statistically significant P<0.05. The difference between Biodentin and NeoPutty groups was not statistically significant P>0.05.
Conclusion
In vital pulp treatments, it can be preferred to use with calcium silicate-based resin-containing TheraCal PT Universal adhesive systems. However, further histological studies are needed to evaluate its effects on vital pulp. Although the shear bond values of NeoPutty were lower than Biodentin, this result was not statistically significant. It was thought that in vivo studies are needed to understand the bonding mechanism of adhesive systems to NeoPutty in composite restorations.

Keywords

Universal adhesive system, Biomaterial, Shear bond strength, Vital pulp treatment

Biomateryallerin Üniversal Adeziv sistemlere Bağlanma Dayanımının Karşılaştırılması: İn Vitro Çalışma

Abstract

Amaç: Bu çalışmanın amacı, kalsiyum silikat içerikli biomateryallerin farklı pH sahip Üniversal adeziv sistemlere makaslama bağlanma dayanımını karşılaştırmalı olarak değerlendirilmesidir.
Gereç ve Yöntem
20 milimetre (mm) çapında ve 20 mm derinlikte kare kalıplara silindirik boşlukları(4mm çap- 2 mm derinlik) bulunan 63 adet akrilik blok hazırlandı. Dokuz gruba ayrıldı. Üretici firmaların talimatları doğrultusunda her bir biyomateryalden (NeoPutty, Theracal PT, Biodentine) 21 adet olacak şekilde hazırlanan boşluklara yerleştirildi. Biyomateryallerin üzerine Üniversal Adeziv sistemler; Gluma Bond Universal, Single Bond Universal, G-Premio Bond üretici firma talimatına göre uygulandıktan sonra kompozit rezin polietilenden hazırlanmış 2 mm çapında ve 2 mm yüksekliğinde silindirik plastik tüplere yerleştirildi ve 20 sn LED ışık cihazı ile polimerize edildi. Tüm örneklerin makaslama bağlanma dayanımı universal test cihazında Newton cinsinden ölçüldü. Shapiro-wilk testi sonucuna göre istatistiksel Parametrik testlerden Tek Yönlü (one way ANOVA) Varyans Analizi yöntemi uygulandı. Gruplar kendi aralarında Post hoc Tukey HSD testi ile karşılaştırıldı.
Bulgular
Biodentin ve TheraCal PT grupları arasındaki fark istatistiksel olarak anlamlı bulundu P<0.05. Theracal PT ve NeoPutty grupları arasındaki fark istatistiksel olarak anlamlı bulundu P<0.05. Biodentin ve NeoPutty grupları arasındaki fark istatistiksel olarak anlamlı değil P>0.05.
Sonuç
Vital pulpa tedavilerinde kalsiyum silikat esaslı rezin içerikli TheraCal PT Üniversal adeziv sistemlerle kullanımı tercih edilebilir. Ancak vital pulpa üzerine etkilerini değerlendiren ileri histolojik çalışmalara ihtiyaç duyulmaktadır. NeoPutty’nin gösterdiği makaslama bağlanma değerleri Biodentin’den düşük olmasına rağmen bu sonuç istatistiksel olarak anlamlı değildi. Kompozit restorasyonlarda adeziv sistemlerin NeoPutty'ye bağlanma mekanizmasını anlamak için invivo çalışmalara ihtiyaç olduğu düşünüldü.

Keywords

Üniversal adeziv sistem, Biomateryal, Makaslama bağlanma dayanımı, Vital pulpa tedavisi

References

• 1. Kunert M, Lukomska-Szymanska M. Bio-inductive materials in direct and indirect pulp capping—a review article. Materials 2020; 13:5: 1204.
• 2. Andrei M, Vacaru RP, Coricovac A, Ilinca R, Didilescu AC, Demetrescu I. The effect of calcium-silicate cements on reparative dentinogenesis following direct pulp capping on animal models. Molecules 2021; 26:9: 2725.
• 3. Palma PJ, Marques JA, Antunes M, Falacho RI, Sequeira D, Roseiro L, et al. Effect of restorative timing on shear bond strength of composite resin/calcium silicate–based cements adhesive interfaces. Clinical Oral Investigations 2021; 25:5: 3131-3139.
• 4. Fathy SM. Remineralization ability of two hydraulic calcium-silicate based dental pulp capping materials: Cell-independent model. Journal of clinical and experimental dentistry 2019; 11:4: e360.
• 5. Gandolfi MG, Siboni F, Botero T, Bossù M, Riccitiello F, Prati C. Calcium silicate and calcium hydroxide materials for pulp capping: biointeractivity, porosity, solubility and bioactivity of current formulations. Journal of applied biomaterials & functional materials 2015; 13:1: 43-60.
• 6. Li X, Yoshihara K, De Munck J, Cokic S, Pongprueksa P, Putzeys E, et al. Modified tricalcium silicate cement formulations with added zirconium oxide. Clinical oral investigations 2017; 21:3:895-905.
• 7. Daneshpoor N, Pishevar L. Comparative evaluation of bioactive cements on biomimetic remineralization of dentin. Journal of clinical and experimental dentistry 2020;12:3: e291.
• 8. Natale LC, Rodrigues MC, Xavier TA, Simões A, De Souza DN, Braga RR. Ion release and mechanical properties of calcium silicate and calcium hydroxide materials used for pulp capping. International endodontic journal 2015; 48:1:89-94.
• 9. Primus C, Gutmann JL, Tay FR, Fuks AB. Calcium silicate and calcium aluminate cements for dentistry reviewed. Journal of the American Ceramic Society 2022;105:3:1841-1863.
• 10. Mutluay AT, Mutluay M. Characterisation of the calcium silicate‐based cement–composite interface and the bonding strength with total‐etch or single/two‐stage self‐etch adhesive systems. Australian Endodontic Journal 2021; doi: 10.1111/aej.12600.
• 11. Sun Q, Meng M, Steed JN, Sidow SJ, Bergeron BE, Niu LN. Manoeuvrability and biocompatibility of endodontic tricalcium silicate-based putties. Journal of Dentistry 2021; 104: 103530.
• 12. İpek İ, Ünal M, Güner A, Candan M. Push-out bond strength of Biodentine, MTA repair HP, and a new pre-mixed NeoPutty bioactive cement: scanning electron microscopy energy dispersive X-ray spectroscopy analysis. Journal of the Australian Ceramic Society 2022; 58:1: 171-179.
• 13. Sun Q, Gustin JW, Tian FC, Sidow SJ, Bergeron BE, Ma JZ, et al. Effects of pre-mixed hydraulic calcium silicate putties on osteogenic differentiation of human dental pulp stem cells in vitro. Journal of Dentistry 2021; 108: 103653.
• 14. NuSmile. Available at: https://www.nusmi le.com/NeoPu tty/ Techn ical-Support.Accessed on 01 October 2021.
• 15. Sanz JL, Soler-Doria A, López-García S, García-Bernal D, Rodríguez-Lozano FJ, Lozano A, et al. Comparative Biological Properties and Mineralization Potential of 3 Endodontic Materials for Vital Pulp Therapy: Theracal PT, Theracal LC, and Biodentine on Human Dental Pulp Stem Cells. Journal of Endodontics 2021; 47:12: 1896-1906.
• 16. Nam OH, Kim JH, Choi SC, Kim Y. Time-dependent response of human deciduous tooth-derived dental pulp cells treated with TheraCal LC: functional analysis of gene interactions compared to MTA. Journal of clinical medicine 2020; 9:2: 531.
• 17. Karadas M, Atıcı MG. Bond strength and adaptation of pulp capping materials to dentin. Microscopy Research and Technique 2020; 83:5: 514-522.
• 18. Sismanoglu S, Yildirim-Bilmez Z, Gurcan AT, Gumustas B. Influence of application mode of universal adhesive on the surface morphology, elemental composition and bond strength of calcium silicate-based cements to composite resin: a SEM-EDX microanalysis study. Journal of Adhesion Science and Technology 2021;1-14. https://doi.org/10.1080/01694243.2021.1992979
• 19. Özata MY, Falakaloğlu S, Plotino G, Adıgüzel Ö. The micro‐shear bond strength of new endodontic tricalcium silicate‐based putty: An in vitro study. Australian Endodontic Journal 2022; https://doi.org/10.1111/aej.12631
• 20. Shafiei F, Doozandeh M, Gharibpour F, Adl A. Effect of reducing acid‐etching duration time on compressive strength and bonding of a universal adhesive to calcium silicate cements. International Endodontic Journal 2019;52:4: 530-539.
• 21. Xavier MT, Costa AL, Caramelo FJ, Palma PJ, Ramos JC. Evaluation of the Interfaces between Restorative and Regenerative Biomaterials Used in Vital Pulp Therapy. Materials 2021; 14:17: 5055.
• 22. Kalyoncuoğlu E, Keskin C, Acar DH, Gonulol N. The bond strength of universal adhesives with different acidities to calcium silicate-based materials. Clinical and Experimental Health Sciences 2021; 11:1: 170-174.
• 23. Yamauchi K, Tsujimoto A, Jurado CA, Shimatani Y, Nagura Y, Takamizawa T, et al. Etch-and-rinse vs self-etch mode for dentin bonding effectiveness of universal adhesives. Journal of oral science 2019; 18-0433.
• 24. Carrilho E, Cardoso M, Marques Ferreira M, Marto CM, Paula A, Coelho AS. (2019). 10-MDP based dental adhesives: adhesive interface characterization and adhesive stability—a systematic review. Materials 2019; 12:5: 790.
• 25. Cetin AR, Dinc H. Effects of artificial aging on the bond strengths of universal dental adhesives. Nigerian Journal of Clinical Practice 2020; 23:8: 1148-1154.
• 26. Choi AN, Lee JH, Son S, Jung KH, Kwon YH, Park JK. Effect of dentin wetness on the bond strength of universal adhesives. Materials 2017;10:11: 1224.
• 27. Deepa VL, Dhamaraju B, Bollu IP, Balaji TS. Shear bond strength evaluation of resin composite bonded to three different liners: TheraCal LC, Biodentine, and resin-modified glass ionomer cement using universal adhesive: An in vitro study. Journal of Conservative Dentistry: JCD 2016; 19:2: 166-170.
• 28. Akbiyik SY, Bakir EP, Bakir SE. Evaluation of the bond strength of different pulp capping materials to dental adhesive systems: an in vitro study. Journal of Advanced Oral Research 2021; 12:2: 286-295.
• 29. Kudva A, Raghunath A, Nair PM, Shetty HK, D’Costa VF, Jayaprakash K. Comparative evaluation of shear bond strength of a bioactive material to composite resin using three different universal bonding agents: An in vitro study. Journal of Conservative Dentistry: JCD 2022; 25:1: 54-57.
• 30. Ashofteh Yazdi K, Ghabraei S, Bolhari B, Kafili M, Meraji N, Nekoofar MH, et al. Microstructure and chemical analysis of four calcium silicate-based cements in different environmental conditions. Clinical oral investigations 2019; 23:1:43-52.
• 31. Laurent P, Camps J, About I. BiodentineTM induces TGF‐β1 release from human pulp cells and early dental pulp mineralization. International endodontic journal 2012; 45:5: 439-448.
• 32. Rendžova V, Apostolska S, Kostadinovska E, Antanasova M, Eftimoska M, Petkov M, et al. Evaluation of bond strength of one step and two steps self-etch adhesive agents with two different pulp-capping materials. Stomatoloski glasnik Srbije 2020; 67: 2: 75-82.
• 33. Odabaş ME, Bani M, Tirali RE. Shear bond strengths of different adhesive systems to biodentine. The Scientific World Journal 2013; http://dx.doi.org/10.1155/2013/626103
• 34. Tohidkhah S, Ahmadi E, Abbasi M, Morvaridi Farimani R, Ranjbar Omrani L. Effect of Bioinductive Cavity Liners on Shear Bond Strength of Dental Composite to Dentin. BioMed Research International 2022; https://doi.org/10.1155/2022/3283211
• 35. Aksoy S, Ünal M. Shear bond strength of universal adhesive systems to a bioactive dentin substitute (Biodentine®) at different time intervals. Stomatological Disease and Science 2017; 1: 116-122.