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Diyot lazer uygulamasının kök yüzeyi karbon, kalsiyum ve fosfor seviyelerine etkisi: Bir SEM-EDX çalışması

Year 2019, Volume: 6 Issue: 2, 163 - 168, 01.08.2019
https://doi.org/10.15311/selcukdentj.521879

Abstract

Amaç: Periodontal tedavi sırasında kök yüzeylerindeki
etkilenmemiş organik unsurların korunması gerekliliği literatürde vurgulanmaktadır.
SEM-EDX analizi ile karbon, kalsiyum ve fosfor element seviyelerinin
değerlendirilmesi, kök yüzeyindeki organik ve inorganik yapıların tayininde
uygulanan bir yaklaşımdır. Bu çalışmanın amacı, periodontal cep
dekontaminasyonu enerji düzeyinde gerçekleştirilen diyot lazer uygulamasının
kök yüzeyindeki organik içeriğe etkisinin SEM-EDX yöntemi ile incelenmesidir.

Gereç ve Yöntemler:Ortodontik nedenlerden ötürü çekilmiş olan
toplam 10 adet dişin her bir aproksimal yüzeyi test (n=20) ve kontrol (n=20) alanları
olmak üzere iki bölgeye ayrıldı. Test bölgelerine diyot lazer (810 nm)
uygulamaları gerçekleştirildi. Bütün örnekler karbon, kalsiyum ve fosfor
element seviyeleri açısından SEM-EDX yöntemi ile analiz edildi.

Bulgular: Diyot lazer uygulanan kök yüzeylerinde, kontrol
alanlarına göre anlamlı düzeyde düşük karbon seviyeleri gözlendi. Kalsiyum ve
fosfor seviyeleri test bölgelerinde kontrol alanlarında göre daha yüksek
seviyede gözlendi.

Sonuçlar: Bu çalışmanın sınırları kapsamında, diyot lazer
uygulamalarının kök yüzeyindeki organik yapıya etki edebileceği ortaya
konmuştur. Diyot lazerinin kök yüzeyindeki spesifik protein yapılarına olan
etkilerini inceleyen sonraki çalışmalara ihtiyaç vardır.

Anahtar Kelimeler: Diyod lazer; kök yüzeyi; SEM-EDX; element
analizi

References

  • Referans1. Rossmann JA, Cobb CM. Lasers in periodontal therapy. Periodontol 2000 1995;9:150-164.
  • Referans2. Parker S. Lasers and soft tissue: periodontal therapy. Br Dent J 2007;202:309-315.
  • Referans3. Passanezi E, Damante CA, de Rezende ML, Greghi SL. Lasers in periodontal therapy. Periodontol 2000 2015;67:268-291.
  • Referans4. Greenstein G. Periodontal response to mechanical non-surgical therapy: a review. J Periodontol 1992;63:118-130.
  • Referans5. Qadri T, Javed F, Johannsen G, Gustafsson A. Role of diode lasers (800-980 nm) as adjuncts to scaling and root planing in the treatment of chronic periodontitis: a systematic review. Photomed Laser Surg 2015;33:568-575.
  • Referans6. Gehrig JS, Sroda R, Saccuzzo D. Fundamentals of periodontal instrumentation & advanced root instrumentation: p.
  • Referans7. Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing and regeneration. Crit Rev Oral Biol Med 2002;13:474-484.
  • Referans8. MacNeil RL, Somerman MJ. Molecular factors regulating development and regeneration of cementum. J Periodontal Res 1993;28:550-559.
  • Referans9. Maruyama H, Aoki A, Sasaki KM, et al. The effect of chemical and/or mechanical conditioning on the Er:YAG laser-treated root cementum: analysis of surface morphology and periodontal ligament fibroblast attachment. Lasers Surg Med 2008;40:211-222.
  • Referans10. Ayo-Yusuf OA, Driessen CH, Botha AJ. SEM-EDX study of prepared human dentine surfaces exposed to gingival retraction fluids. J Dent 2005;33:731-739.
  • Referans11. Euzebio Alves VT, de Andrade AK, Toaliar JM, et al. Clinical and microbiological evaluation of high intensity diode laser adjutant to non-surgical periodontal treatment: a 6-month clinical trial. Clin Oral Investig 2013;17:87-95.
  • Referans12. Brudevold F, Steadman LT, Smith FA. Inorganic and organic components of tooth structure. Ann N Y Acad Sci 1960;85:110-132.
  • Referans13. LeGeros RZ. Calcium phosphates in oral biology and medicine. Monogr Oral Sci 1991;15:1-201.
  • Referans14. Ho SP, Yu B, Yun W, Marshall GW, Ryder MI, Marshall SJ. Structure, chemical composition and mechanical properties of human and rat cementum and its interface with root dentin. Acta Biomater 2009;5:707-718.
  • Referans15. Macneil RL, Sheng N, Strayhorn C, Fisher LW, Somerman MJ. Bone Sialoprotein Is Localized to the Root Surface during Cementogenesis. Journal of Bone and Mineral Research 1994;9:1597-1606.
  • Referans16. Proye MP, Polson AM. Effect of root surface alterations on periodontal healing. I. Surgical denudation. J Clin Periodontol 1982;9:428-440.
  • Referans17. Blomlof JP, Blomlof LB, Lindskog SF. Smear removal and collagen exposure after non-surgical root planing followed by etching with an EDTA gel preparation. J Periodontol 1996;67:841-845.
  • Referans18. Polson AM, Caton J. Factors influencing periodontal repair and regeneration. J Periodontol 1982;53:617-625.
  • Referans19. Schwarz F, Sculean A, Georg T, Reich E. Periodontal treatment with an Er: YAG laser compared to scaling and root planing. A controlled clinical study. J Periodontol 2001;72:361-367.
  • Referans20. Morlock BJ, Pippin DJ, Cobb CM, Killoy WJ, Rapley JW. The effect of Nd:YAG laser exposure on root surfaces when used as an adjunct to root planing: an in vitro study. J Periodontol 1992;63:637-641.
  • Referans21. Kreisler M, Al Haj H, Daublander M, et al. Effect of diode laser irradiation on root surfaces in vitro. J Clin Laser Med Surg 2002;20:63-69.
  • Referans22. Theodoro LH, Haypek P, Bachmann L, et al. Effect of ER:YAG and diode laser irradiation on the root surface: morphological and thermal analysis. J Periodontol 2003;74:838-843.
  • Referans23. Henmi A, Okata H, Anada T, et al. Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX spectroscopy, XRD, and FTIR spectroscopy. J Bone Miner Metab 2016;34:41-50.
  • Referans24. Arnold WH, Gaengler P. Quantitative analysis of the calcium and phosphorus content of developing and permanent human teeth. Ann Anat 2007;189:183-190.

Effect of diode laser application on root surface carbon, calcium and phosphorus levels: A SEM-EDX study

Year 2019, Volume: 6 Issue: 2, 163 - 168, 01.08.2019
https://doi.org/10.15311/selcukdentj.521879

Abstract

Background:Preservation of the organic ingredients on the
root surfaces during the periodontal therapy has been emphasized in the
literature. SEM-EDX analysis is an accepted approach to evaluate organic and
inorganic components on investigated samples in terms of carbon, calcium and
phosphor levels. The aim of this study was to evaluate the effects of diode
laser application with periodontal pocket decontamination energy setting to the
organic content of intact root surfaces via SEM-EDX analysis.

Methods: Each proximal root surfaces of the root surfaces
of 10 teeth which were extracted due to orthodontic reasons were divided as
test (n=20) and control (n=20) sites. Diode laser (810 nm) applications were done
to the test sites of root surfaces. All specimens were analyzed using SEM-EDX
with respect to carbon, calcium and phosphor levels.

Results:Diode laser applied root surfaces exhibit significantly
lower carbon levels comparing with control sites. Additionally, increased
calcium and phosphor levels were detected in the test sites.

Conclusions:Within the limitations of this study, it can be
concluded that, diode laser applications may alter organic content of intact root
surface. Further studies are required to elucidate potential effects of diode
laser application to specific protein components
on the root surfaces.

Keywords: Diode laser, root surface, SEM-EDX, element
levels
          

References

  • Referans1. Rossmann JA, Cobb CM. Lasers in periodontal therapy. Periodontol 2000 1995;9:150-164.
  • Referans2. Parker S. Lasers and soft tissue: periodontal therapy. Br Dent J 2007;202:309-315.
  • Referans3. Passanezi E, Damante CA, de Rezende ML, Greghi SL. Lasers in periodontal therapy. Periodontol 2000 2015;67:268-291.
  • Referans4. Greenstein G. Periodontal response to mechanical non-surgical therapy: a review. J Periodontol 1992;63:118-130.
  • Referans5. Qadri T, Javed F, Johannsen G, Gustafsson A. Role of diode lasers (800-980 nm) as adjuncts to scaling and root planing in the treatment of chronic periodontitis: a systematic review. Photomed Laser Surg 2015;33:568-575.
  • Referans6. Gehrig JS, Sroda R, Saccuzzo D. Fundamentals of periodontal instrumentation & advanced root instrumentation: p.
  • Referans7. Grzesik WJ, Narayanan AS. Cementum and periodontal wound healing and regeneration. Crit Rev Oral Biol Med 2002;13:474-484.
  • Referans8. MacNeil RL, Somerman MJ. Molecular factors regulating development and regeneration of cementum. J Periodontal Res 1993;28:550-559.
  • Referans9. Maruyama H, Aoki A, Sasaki KM, et al. The effect of chemical and/or mechanical conditioning on the Er:YAG laser-treated root cementum: analysis of surface morphology and periodontal ligament fibroblast attachment. Lasers Surg Med 2008;40:211-222.
  • Referans10. Ayo-Yusuf OA, Driessen CH, Botha AJ. SEM-EDX study of prepared human dentine surfaces exposed to gingival retraction fluids. J Dent 2005;33:731-739.
  • Referans11. Euzebio Alves VT, de Andrade AK, Toaliar JM, et al. Clinical and microbiological evaluation of high intensity diode laser adjutant to non-surgical periodontal treatment: a 6-month clinical trial. Clin Oral Investig 2013;17:87-95.
  • Referans12. Brudevold F, Steadman LT, Smith FA. Inorganic and organic components of tooth structure. Ann N Y Acad Sci 1960;85:110-132.
  • Referans13. LeGeros RZ. Calcium phosphates in oral biology and medicine. Monogr Oral Sci 1991;15:1-201.
  • Referans14. Ho SP, Yu B, Yun W, Marshall GW, Ryder MI, Marshall SJ. Structure, chemical composition and mechanical properties of human and rat cementum and its interface with root dentin. Acta Biomater 2009;5:707-718.
  • Referans15. Macneil RL, Sheng N, Strayhorn C, Fisher LW, Somerman MJ. Bone Sialoprotein Is Localized to the Root Surface during Cementogenesis. Journal of Bone and Mineral Research 1994;9:1597-1606.
  • Referans16. Proye MP, Polson AM. Effect of root surface alterations on periodontal healing. I. Surgical denudation. J Clin Periodontol 1982;9:428-440.
  • Referans17. Blomlof JP, Blomlof LB, Lindskog SF. Smear removal and collagen exposure after non-surgical root planing followed by etching with an EDTA gel preparation. J Periodontol 1996;67:841-845.
  • Referans18. Polson AM, Caton J. Factors influencing periodontal repair and regeneration. J Periodontol 1982;53:617-625.
  • Referans19. Schwarz F, Sculean A, Georg T, Reich E. Periodontal treatment with an Er: YAG laser compared to scaling and root planing. A controlled clinical study. J Periodontol 2001;72:361-367.
  • Referans20. Morlock BJ, Pippin DJ, Cobb CM, Killoy WJ, Rapley JW. The effect of Nd:YAG laser exposure on root surfaces when used as an adjunct to root planing: an in vitro study. J Periodontol 1992;63:637-641.
  • Referans21. Kreisler M, Al Haj H, Daublander M, et al. Effect of diode laser irradiation on root surfaces in vitro. J Clin Laser Med Surg 2002;20:63-69.
  • Referans22. Theodoro LH, Haypek P, Bachmann L, et al. Effect of ER:YAG and diode laser irradiation on the root surface: morphological and thermal analysis. J Periodontol 2003;74:838-843.
  • Referans23. Henmi A, Okata H, Anada T, et al. Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX spectroscopy, XRD, and FTIR spectroscopy. J Bone Miner Metab 2016;34:41-50.
  • Referans24. Arnold WH, Gaengler P. Quantitative analysis of the calcium and phosphorus content of developing and permanent human teeth. Ann Anat 2007;189:183-190.
There are 24 citations in total.

Details

Primary Language English
Subjects Dentistry
Journal Section Research
Authors

Emre Yaprak 0000-0001-7797-9796

Publication Date August 1, 2019
Submission Date February 4, 2019
Published in Issue Year 2019 Volume: 6 Issue: 2

Cite

Vancouver Yaprak E. Effect of diode laser application on root surface carbon, calcium and phosphorus levels: A SEM-EDX study. Selcuk Dent J. 2019;6(2):163-8.