Chlorophyll, Carotene, and Anthocyanin Effect on Color Change of Resin-Based Dental Materials: An in-Vitro Study

Year 2024, Volume: 11 Issue: 2, 146 - 151, 19.08.2024

Bahar Elter , Burcu Diker

https://doi.org/10.15311/selcukdentj.1361209

Abstract

Background: The purpose of the present study was to evaluate the effect of chlorophyll, carotene, and anthocyanin-containing juices on color stability of resin-based dental materials.
Methods: 192 disc-shaped specimens were prepared from six resin-based materials (universal, anterior, and flowable resin composites; self-curing polymethyl methacrylate (pmma), bis-acrylic composite, and computer-aided design/computer-aided manufacturing(CAD/CAM)- fabricated pmma). Divided groups were immersed in distilled water, spinach, carrot, and black mulberry juices (n=8). Color measurements were taken with a spectrophotometer before the immersions(T0), after one week(T1), and after four weeks(T2). The color differences (ΔE00) were used for statistical analysis. ANOVA was used to determine the effects of material, solution, and time on color change.
Results: The highest color change was recorded for the CAD/CAM-fabricated pmma (ΔE=2.67) (p<0.05). Distilled water was the least coloring solution (ΔE=0.60), and carrot juice was the highest (ΔE=2.37). No significant difference was seen between black mulberry and carrot juice (p=0.071). The mean ΔE value was 1.84 in T0-T1 and 1.43 in T1-T2 (p<0.001).
Conclusions: Carrot was the highest colorant with black mulberry, giving clinically unacceptable ΔE00 values. Spinach caused only visually perceptible coloration. All materials got visually perceptible color change after the juice immersions. Only CAD/CAM-fabricated pmma showed clinically unacceptable ΔE00 values.

Keywords

dental materials, composit resins, coloring agents, dental restoration temporary, dental restoration permanent

Klorofil, Karoten ve Antosiyaninin Rezin Bazlı Dental Materyallerdeki Renk Değişimine Etkisi: in Vitro Çalışma

Abstract

Amaç: Bu çalışmanın amacı; klorofil, karoten ve antosiyanin içeren bitki sularının, rezin bazlı dental materyallerin renk stabilitesi üzerindeki etkisinin ölçülmesidir.
Gereç ve Yöntemler: Altı farklı rezin bazlı dental materyalden (universal, anterior ve akışkan rezin kompozit; kendi kendine sertleşen polimetil metakrilat (pmma), bis-akrilik kompozit ve bilgisayar destekli tasarım/bilgisayar destekli üretim (CAD/CAM ) ile üretilen pmma) 192 disk şeklinde örnek hazırlandı. Örnek grupları distile su, ıspanak, havuç ve karadut sularına (n=8) daldırıldı. Daldırmalardan önce (T0), bir hafta sonra (T1) ve dört hafta sonra (T2) spektrofotometre ile renk ölçümleri yapıldı. İstatistiksel analiz için renk farklılık değerleri (ΔE00) kullanıldı. Materyalin, solüsyonun ve zamanın renk değişimi üzerindeki etkilerini belirlemek için ANOVA kullanıldı.
Bulgular: En fazla renk değişimi CAD/CAM ile üretilen pmma'da kaydedildi (ΔE=2.67) (p<0,05). En az renk değişimi distile suda (ΔE=0.60), en yüksek renk değişimi havuç suyunda kaydedildi (ΔE=2,37). Karadut ve havuç suyu arasında anlamlı fark görülmedi (p=0.071). Ortalama ΔE değeri T0-T1'de 1.84, T1-T2'de 1.43 idi (p<0,001).
Sonuç: Havuç, karadut ile birlikte en yüksek renklendirici olup, klinik olarak kabul edilemez ΔE00 değerleri verdi. Ispanak yalnızca görsel olarak algılanabilen bir renklenmeye neden oldu. Tüm materyaller bitki sularında bekletildikten sonra görsel olarak algılanabilir bir renk değişimine uğradı. Yalnızca CAD/CAM ile üretilmiş pmma, klinik olarak kabul edilemez ΔE00 değerleri gösterdi.

Keywords

kompozit rezinler, boyayıcı ajanlar, geçici restorasyonlar, kalıcı restorasyonlar, dental materyaller

References

• 1. Liu RH. Dietary bioactive compounds and their health implications. J Food Sci. 2013 Jun;78 Suppl 1:A18-25.
• 2. Kim MJ, Jun JG, Park SY, Choi MJ, Park E, Kim JI, et al. Antioxidant activities of fresh grape juices prepared using various household processing methods. Food Sci Biotechnol. 2017;26(4):861–9.
• 3. Durazzo A, Lucarini M, Novellino E, Daliu P, Santini A. Fruit-based juices: Focus on antioxidant properties-Study approach and update. Phytother Res. 2019 Jul;33(7):1754–69.
• 4. Choi S, Park S, Hong S, Shin H, Jung BK, Kim MJ. Green vegetable juice as a potential source of human fascioliasis in Korea. One Heal [Internet]. 2022;15:100441. Available from: https://www.sciencedirect.com/science/article/pii/S2352771422000738
• 5. Fernández-García E, Carvajal-Lérida I, Jarén-Galán M, Garrido-Fernández J, Pérez-Gálvez A, Hornero-Méndez D. Carotenoids bioavailability from foods: From plant pigments to efficient biological activities. Food Res Int [Internet]. 2012;46(2):438–50. Available from: https://www.sciencedirect.com/science/article/pii/S096399691100370X
• 6. Ferruzzi MG, Blakeslee J. Digestion, absorption, and cancer preventative activity of dietary chlorophyll derivatives. Nutr Res [Internet]. 2007;27(1):1–12. Available from: https://www.sciencedirect.com/science/article/pii/S0271531706002934
• 7. Koley TK, Nishad J, Kaur C, Su Y, Sethi S, Saha S, et al. Effect of high-pressure microfluidization on nutritional quality of carrot (Daucus carota L.) juice. J Food Sci Technol [Internet]. 2020;57(6):2159–68. Available from: https://doi.org/10.1007/s13197-020-04251-6
• 8. Li D, Wang P, Luo Y, Zhao M, Chen F. Health benefits of anthocyanins and molecular mechanisms: Update from recent decade. Crit Rev Food Sci Nutr [Internet]. 2017 May 24;57(8):1729–41. Available from: https://doi.org/10.1080/10408398.2015.1030064
• 9. Demir Doğan M, Can G, Meral R. Effectiveness of Black Mulberry Molasses in Prevention of Radiotherapy-Induced Oral Mucositis: A Randomized Controlled Study in Head and Neck Cancer Patients. J Altern Complement Med. 2017 Dec;23(12):971–9.
• 10. Korkut S, Karadağ S, Levent Çınar S. The Effect of Oral Care with Black Mulberry Syrup on Oral Mucositis in Patients With COPD: A Mixed Study. Altern Ther Health Med. 2023 Mar;29(2):132–9.
• 11. Paolone G, Mandurino M, De Palma F, Mazzitelli C, Scotti N, Breschi L, et al. Color Stability of Polymer-Based Composite CAD/CAM Blocks: A Systematic Review. Polymers (Basel). 2023 Jan;15(2).
• 12. Paolone G, Formiga S, De Palma F, Abbruzzese L, Chirico L, Scolavino S, et al. Color stability of resin-based composites: Staining procedures with liquids-A narrative review. J Esthet Restor Dent Off Publ Am Acad Esthet Dent. [et al]. 2022 Sep;34(6):865–87.
• 13. Mazaro JVQ, Minani LM, Zavanelli AC, Mello CC de, Lemos CAA. Evaluation of color stability of different temporary restorative materials. Rev Odontol da UNESP [Internet]. 2015 Sep;44(Rev. odontol. UNESP, 2015 44(5)). Available from: https://doi.org/10.1590/1807-2577.0017
• 14. Costa ÍA da F, Lima EMCX. Effect of colorant solutions on the color stability of provisional prosthetic materials. Brazilian J Oral Sci [Internet]. 2018;17:e18153. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8652654
• 15. Haselton DR, Diaz-Arnold AM, Dawson D V. Color stability of provisional crown and fixed partial denture resins. J Prosthet Dent [Internet]. 2005;93(1):70–5. Available from: https://www.sciencedirect.com/science/article/pii/S0022391304006250
• 16. Ertas E, Guler AU, Yucel AC, Koprulu H, Guler E. Color stability of resin composites after immersion in different drinks. Dent Mater J. 2006/08/19. 2006;25(2):371–6.
• 17. Kim-Pusateri S, Brewer JD, Davis EL, Wee AG. Reliability and accuracy of four dental shade-matching devices. J Prosthet Dent [Internet]. 2009;101(3):193–9. Available from: http://www.sciencedirect.com/science/article/pii/S0022391309600287
• 18. CIE: Commission International de l’Eclairage (CIE): Colorimetry (ed 3). CIE publication No. 15. Vienna, Austria, Central Bureau of the CIE, 2004.
• 19. Luo MR, Cui G, Rigg B. The development of the CIE 2000 colour-difference formula: CIEDE2000. Color Res Appl [Internet]. 2001 Oct 1;26(5):340–50. Available from: https://doi.org/10.1002/col.1049
• 20. Paravina RD, Pérez MM, Ghinea R. Acceptability and perceptibility thresholds in dentistry: A comprehensive review of clinical and research applications. J Esthet Restor Dent [Internet]. 2019;31(2):103–12. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1111/jerd.12465
• 21. Paravina RD, Ghinea R, Herrera LJ, Bona AD, Igiel C, Linninger M, et al. Color Difference Thresholds in Dentistry. J Esthet Restor Dent [Internet]. 2015 Mar 1;27(S1):S1–9. Available from: https://doi.org/10.1111/jerd.12149
• 22. Topcu FT, Sahinkesen G, Yamanel K, Erdemir U, Oktay EA, Ersahan S. Influence of different drinks on the colour stability of dental resin composites. Eur J Dent. 2009 Jan;3(1):50–6.
• 23. Yikilgan İ, Akgul S, Hazar A, Kedıcı Alp C, Baglar S, Bala O. The Effects of Fresh Detox Juices on Color Stability and Roughness of Resin-Based Composites. J Prosthodont Off J Am Coll Prosthodont. 2019 Jan;28(1):e82–8.
• 24. Luo M. The CIE 2000 colour difference formula: CIEDE2000. Color Res Appl. 2002 Jun 1;4421.
• 25. Yu B, Lee YK. Comparison of the color stability of flowable and universal resin composites. Am J Dent. 2009 Jun;22(3):160–4.
• 26. Neo Spectra ST Flow technical documentation, https://www.dentsplysirona.com/tr-tr/kesfet/restoratif/neo-spectra-st-family/neo-spectra-st-flow.html
• 27. Manojlovic D, Lenhardt L, Milićević B, Antonov M, Miletic V, Dramićanin MD. Evaluation of Staining-Dependent Colour Changes in Resin Composites Using Principal Component Analysis. Sci Rep. 2015 Oct;5:14638.
• 28. Luiz BKM, Amboni RDMC, Prates LHM, Roberto Bertolino J, Pires ATN. Influence of drinks on resin composite: Evaluation of degree of cure and color change parameters. PolymTest[Internet].2007;26(4):438–44.Availablefrom: https://www.sciencedirect.com/science/article/pii/S0142941806002200
• 29. Abu-Bakr N, Han L, Okamoto A, Iwaku M. Color stability of compomer after immersion in various media. J Esthet Dent. 2000;12(5):258–63.
• 30. Motayagheni R, Ebrahim Adhami Z, Taghizadeh Motlagh SM, Mehrara F, Yasamineh N. Color changes of three different brands of acrylic teeth in removable dentures in three different beverages: An in vitro study. J Dent Res Dent Clin Dent Prospects. 2020;14(3):159–65.
• 31. Antonov M, Lenhardt L, Manojlovic D, Milicevic B, Dramićanin M. Discoloration of resin-based compounds in natural juices and energy drinks. Vojn Pregl. 2018;75(8):787–94.
• 32. Elagra MI, Rayyan MR, Alhomaidhi MM, Alanaziy AA, Alnefaie MO. Color stability and marginal integrity of interim crowns: An in vitro study. Eur J Dent. 2017;11(3):330–4.
• 33. Peñate L, Mercade M, Arregui M, Roig M, Basilio J, Cedeño R. Color Stability of CAD/CAM Interim Material for Long-Term Fixed Dental Prostheses vs. Conventional Materials after Immersion in Different Staining Solutions. Vol. 5, Journal of Composites Science. 2021.
• 34. Yannikakis SA, Zissis AJ, Polyzois GL, Caroni C. Color stability of provisional resin restorative materials. J Prosthet Dent [Internet]. 1998;80(5):533–9. Available from: https://www.sciencedirect.com/science/article/pii/S0022391398700289
• 35. Rayyan MM, Aboushelib M, Sayed NM, Ibrahim A, Jimbo R. Comparison of interim restorations fabricated by CAD/CAM with those fabricated manually. J Prosthet Dent. 2015 Sep;114(3):414–9.