Pulpa Kaplama Biyomateryallerinin Biyoaktivitelerinin Değerlendirilmesi: In vitro Yaklaşımlar

Year 2024, Volume: 11 Issue: 3, 362 - 368, 23.12.2024

Esra Baltacıoğlu Gundogdu , Ramazan Gundogdu

https://doi.org/10.15311/selcukdentj.1494689

Abstract

Diş pulpasının sağlığının korunması, dişin uzun süreli sağlığı için çok önemlidir. Vital pulpa tedavisi travma, çürük veya diş prosedürlerinden etkilenen dişlerde pulpa dokusunun canlılığını korumayı ve desteklemeyi amaçlamaktadır. Birincil amaç, diş canlılığını korumak için ilave dentin oluşumunu teşvik etmektir. Vital pulpa tedavisindeki başarı; doğru teşhise, hasta seçimine ve pulpanın maruz kaldığı yer, pulpa olgunluğu ve mevcut diş tedavisinin kalitesi gibi çeşitli faktörlerin değerlendirilmesine bağlıdır. Vital pulpa tedavisinde, açıkta kalan dentin üzerine pulpa kaplama materyali adı verilen koruyucu bir biyoajan uygulanır. Başarılı doku yanıtlarını teşvik etmek ve hasta sonuçlarını iyileştirmek için çok sayıda diş biyomateryali geliştirilmiştir. Dentin-pulpa kompleksi iyileşme mekanizmalarına ilişkin anlayışımızdaki gelişmelerle birlikte, konservatif ve restoratif diş prosedürleri sırasında pulpa canlılığını destekleyen yeni biyomateryaller ortaya çıkmıştır. Ancak mevcut seçeneklerin çeşitliliği nedeniyle her klinik senaryo için en uygun biyomateryali seçmek zor olabilmektedir. Bu nedenle, pulpa kaplamanın diş sağlığının korunmasındaki önemli rolü göz önüne alındığında, yeni pulpa kaplama biyomateryallerinin biyoaktivitesinin geliştirilmesi ve değerlendirilmesi çok önemlidir. Bu derlemede, yeni pulpa kaplama biyomateryallerinin hem sitoprotektif hem de sitotoksik özelliklerini değerlendirmek için sıklıkla kullanılan tipik in vitro hücre kültürü ve moleküler biyoloji tekniklerini derlenmiştir. Bu teknikler, biyomateryal etkinliğinin ve güvenliğinin kapsamlı bir şekilde değerlendirilmesine katkıda bulunarak hayati pulpal tedavi uygulamalarının ve hasta bakımının geliştirilmesine yardımcı olur.

Keywords

Pulpa kaplama biyomateryalleri, Biyoaktivite, Biyouyumluluk, Hücre Kültürü

Ethical Statement

Yazarlar çıkar çatışması bildirmemiştir.

Supporting Institution

Yazarlar bu çalışma için finansal destek almadığını beyan etmiştir.

Bioactivity Assessment of Pulp Capping Biomaterials: In vitro Approaches

Abstract

Maintaining the health of the dental pulp is essential for the long-term well-being of a tooth. Vital pulpal therapy is aimed at preserving and supporting the vitality of pulp tissue in teeth affected by trauma, caries, or dental procedures. The primary objective is to stimulate the formation of reparative dentin to maintain tooth viability. Successful outcomes in vital pulpal therapy rely on accurate diagnosis, patient selection, and evaluation of various factors such as pulp exposure location, pulp maturity, and quality of existing dental treatment. In vital pulpal therapy, a protective bioagent, called a pulp capping material, is applied over the exposed dentin. Over time, numerous dental biomaterials have been developed to promote safe tissue responses and improve treatment outcomes. With advancements in our understanding of dentin-pulp complex healing mechanisms, new biomaterials have emerged to support pulp vitality during conservative and restorative dental procedures. However, selecting the most suitable biomaterial for each clinical scenario can be challenging due to the array of options available. Therefore, developing and assessing the bioactivity of novel pulp capping biomaterials is crucial, given the significant role of pulp capping in maintaining dental health. In this review, we review the typical in vitro cell culture and molecular biology techniques frequently utilised to evaluate both the cytoprotective and cytotoxic properties of novel pulp capping biomaterials. These techniques contribute to the comprehensive assessment of biomaterial efficacy and safety, aiding in the advancement of vital pulpal therapy practices and patient care.

Keywords

Pulp capping biomaterials, Bioactivity, Biocompability, Cell culture

Ethical Statement

The authors have no conflict of interest to declare

Supporting Institution

The authors declared that this study has received no financial support.

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