Zebrafish, A Model Organism For Research in Dentistry

Year 2025, Volume: 12 Issue: 1, 144 - 148, 21.04.2025

Simge Meşeli , Dilek Tağtekin , Ebru Emekli Alturfan

https://doi.org/10.15311/selcukdentj.1446275

Abstract

Zebrafish (Danio rerio) is a tropical freshwater fish, lives in South Asia, India, and Pakistan. The use of zebrafish as a model organism in embryology and genetic studies of vertebrates was first mentioned by George Streisinger. Zebrafish has become a popular vertebrate model organism for biomedical research due to its numerous advantages. Zebrafish genome has homologues of 70% human genes, 80% of which are associated with human diseases. Zebrafish embryos are transparent, developes rapidly, and the development stages can be mentored easily by a stereomicroscope. Due to its various advantages, zebrafish has been preferred in neuroscience, cancer, pharmacology and toxicity research for years. Recently, it has begun to be used as a model organism in dental research. This review aims to provide information on the use of zebrafish in many fields of dental research, dental materials and tooth formation.

Keywords

zebrafish, model organism, dental research, biocompatibility, toxicity

Ethical Statement

No ethical approval was required

Supporting Institution

none

Thanks

none

ZEBRA BALIĞI, DİŞ HEKİMLİĞİ ARAŞTIRMALARINDA MODEL ORGANİZMA

Abstract

Zebra balığı (Danio rerio), Güney Asya, Hindistan ve Pakistan'da yaşayan tropikal bir tatlı su balığıdır. Zebra balığının omurgalıların embriyolojisi ve genetik çalışmalarında model organizma olarak kullanılmasından ilk kez George Streisinger bahsetmiştir. Son yıllarda zebra balığı, sayısız avantajı nedeniyle biyomedikal araştırmalar için popüler bir omurgalı model organizma haline gelmiştir. Zebra balığı genomunun %70'i insan genlerinin homologlarına sahiptir ve bunların %80'i insan hastalıklarıyla ilişkilidir. Zebra balığı embriyoları şeffaftır, hızlı gelişir ve embryoların gelişim aşamaları stereomikroskopla kolaylıkla takip edilebilir. Zebra balığı, çeşitli avantajlarından dolayı nöroloji, kanser, farmakoloji ve toksisite araştırmalarında yıllardır tercih edilmektedir. Son zamanlarda diş hekimliğine ait araştırmalarda model organizma olarak kullanılmaya başlanmıştır. Bu derleme, zebra balığının dental araştırmalar, dental materyaller ve odontogenezis gibi birçok alanda kullanımı hakkında bilgi vermeyi amaçlamaktadır.

Keywords

zebrabalığı, model organizma, dental araştırma, biyouyumluluk, toksisite

References

• 1. Karaman GE, Emekli-Alturfan E, Akyüz S: Zebrafish; an emerging model organism for studying toxicity and biocompatibility of dental materials. Cellular and Molecular Biology 2020, 66(8):41-46.
• 2. He J-H, Gao J-M, Huang C-J, Li C-Q: Zebrafish models for assessing developmental and reproductive toxicity. Neurotoxicology and teratology 2014, 42:35-42.
• 3. Ohashi AS, de Souza Schacher HR, Pizzato CS, Vianna MR, de Menezes LM: Zebrafish as model for studies in dentistry. Journal of Orthodontic Science 2022, 11.
• 4. Jia H-R, Zhu Y-X, Duan Q-Y, Chen Z, Wu F-G: Nanomaterials meet zebrafish: Toxicity evaluation and drug delivery applications. Journal of Controlled Release 2019, 311:301-318.
• 5. Streisinger G, Walker C, Dower N, Knauber D, Singer F: Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature 1981, 291(5813):293-296.
• 6. Kimmel CB: Genetics and early development of zebrafish. Trends in Genetics 1989, 5:283-288.
• 7. Kimmel CB: Patterning the brain of the zebrafish embryo. Annual review of neuroscience 1993, 16(1):707-732.
• 8. Lieschke GJ, Currie PD: Animal models of human disease: zebrafish swim into view. Nature Reviews Genetics 2007, 8(5):353-367.
• 9. Howe K, Clark MD, Torroja CF, Torrance J, Berthelot C, Muffato M, Collins JE, Humphray S, McLaren K, Matthews L: The zebrafish reference genome sequence and its relationship to the human genome. Nature 2013, 496(7446):498-503.
• 10. Langheinrich U: Zebrafish: a new model on the pharmaceutical catwalk. Bioessays 2003, 25(9):904-912.
• 11. Saleem S, Kannan RR: Zebrafish: an emerging real-time model system to study Alzheimer’s disease and neurospecific drug discovery. Cell death discovery 2018, 4(1):45.
• 12. Ünal İ, Üstündağ ÜV, Ateş PS, Eğilmezer G, Alturfan AA, Yiğitbaşı T, Emekli-Alturfan E: Rotenone impairs oxidant/antioxidant balance both in brain and intestines in zebrafish. International Journal of Neuroscience 2019, 129(4):363-368.
• 13. Meşeli S, Kaplan G, Cansiz D, Üstündağ ÜV, İsmail Ü, Alturfan EE, Yanikoğlu F, Tağtekin D: The biocompatibility of sodium lauryl sulphate on developing zebrafish embryos. Experimed 2021, 11(2):67-72.
• 14. Eryılmaz O, Ateş PS, Ünal İ, Üstündağ ÜV, Bay S, Alturfan AA, Yiğitbaşı T, Emekli‐Alturfan E, Akalın M: Evaluation of the interaction between proliferation, oxidant–antioxidant status, Wnt pathway, and apoptosis in zebrafish embryos exposed to silver nanoparticles used in textile industry. Journal of Biochemical and Molecular Toxicology 2018, 32(1):e22015.
• 15. Mork L, Crump G: Zebrafish craniofacial development: a window into early patterning. Current topics in developmental biology 2015, 115:235-269.
• 16. Wiweger MI, Zhao Z, van Merkesteyn RJ, Roehl HH, Hogendoorn PC: HSPG-deficient zebrafish uncovers dental aspect of multiple osteochondromas. PLoS One 2012, 7(1):e29734.
• 17. Stock DW: Zebrafish dentition in comparative context. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 2007, 308(5):523-549.
• 18. Schulze S, Küchler EC, Scariot R, Baratto-Filho F, Carelli J, Proff P, Kirschneck C: Signaling pathways involved in tooth development: why use zebrafish in dental research? RSBO 2021, 18(1):86-99.
• 19. Meşeli S, Üstündağ ÜV, Ateş PS, Ünal İ, Işık Alturfan E: The biocompatibility of a ginger-containing herbal toothpaste on developing zebrafiish embryos. Journal of Dentistry Indonesia 2023.
• 20. Stachurski P, Świątkowski W, Ciszewski A, Sarna-Boś K, Michalak A: A Short Review of the Toxicity of Dentifrices—Zebrafish Model as a Useful Tool in Ecotoxicological Studies. International Journal of Molecular Sciences 2023, 24(18):14339.
• 21. Zhang Y, Zhang Y, Zheng X, Xu R, He H, Duan X: Grading and quantification of dental fluorosis in zebrafish larva. Archives of Oral Biology 2016, 70:16-23.
• 22. Bartlett J, Dwyer S, Beniash E, Skobe Z, Payne-Ferreira T: Fluorosis: a new model and new insights. Journal of dental research 2005, 84(9):832-836.
• 23. Li M, Cao J, Zhao Y, Wu P, Li X, Khodaei F, Han Y, Wang J: Fluoride impairs ovary development by affecting oogenesis and inducing oxidative stress and apoptosis in female zebrafish (Danio rerio). Chemosphere 2020, 256:127105.
• 24. Li M, Cao J, Chen J, Song J, Zhou B, Feng C, Wang J: Waterborne fluoride exposure changed the structure and the expressions of steroidogenic-related genes in gonads of adult zebrafish (Danio rerio). Chemosphere 2016, 145:365-375.
• 25. Karaman GE, Ünal İ, Beler M, Üstündağ FD, Cansız D, Üstündağ Ü V, Emekli-Alturfan E, Akyüz S: Toothpastes for children and their detergent contents affect molecular mechanisms of odontogenesis in zebrafish embryos. Drug Chem Toxicol 2024, 47(1):15-25.
• 26. Oliveira R, Domingues I, Koppe Grisolia C, Soares AM: Effects of triclosan on zebrafish early-life stages and adults. Environmental Science and Pollution Research 2009, 16(6):679-688.
• 27. Gaur H, Purushothaman S, Pullaguri N, Bhargava Y, Bhargava A: Sodium benzoate induced developmental defects, oxidative stress and anxiety-like behaviour in zebrafish larva. Biochemical and biophysical research communications 2018, 502(3):364-369.
• 28. d’Amora M, Liendo F, Deorsola FA, Bensaid S, Giordani S: Toxicological profile of calcium carbonate nanoparticles for industrial applications. Colloids and Surfaces B: Biointerfaces 2020, 190:110947.
• 29. Yi H, Wang Z, Li X, Yin M, Wang L, Aldalbahi A, El-Sayed NN, Wang H, Chen N, Fan C: Silica nanoparticles target a Wnt signal transducer for degradation and impair embryonic development in zebrafish. Theranostics 2016, 6(11):1810.
• 30. Donaruma LG: Definitions in biomaterials, DF Williams, Ed., Elsevier, Amsterdam, 1987, 72 pp. In.: Wiley Online Library; 1988.
• 31. Kramer AG, Vuthiganon J, Lassiter CS: Bis-GMA affects craniofacial development in zebrafish embryos (Danio rerio). Environmental Toxicology and Pharmacology 2016, 43:159-165.
• 32. Makkar H, Verma SK, Panda PK, Jha E, Das B, Mukherjee K, Suar M: In vivo molecular toxicity profile of dental bioceramics in embryonic Zebrafish (Danio rerio). Chemical research in toxicology 2018, 31(9):914-923.
• 33. Roy M, Bandyopadhyay A, Bose S: Ceramics in bone grafts and coated implants. In: Materials for Bone Disorders. edn.: Elsevier; 2017: 265-314.
• 34. Pratama SF, Ana ID, Retnoaji B: The Effect of Carbonate Hydroxyapatite (CHA) Dental Implant Material on the Early Development of Zebrafish Embryos (Danio rerio). In: 3rd KOBI Congress, International and National Conferences (KOBICINC 2020): 2021: Atlantis Press; 2021: 307-312.
• 35. Karthiga P, Ponnanikajamideen M, Rajendran S, Annadurai G, Rajeshkumar S: Characterization and toxicology evaluation of zirconium oxide nanoparticles on the embryonic development of zebrafish, Danio rerio. Drug and Chemical Toxicology 2018, 42(1):104-111.
• 36. Yang H-C, Pon LA: Toxicity of metal ions used in dental alloys: a study in the yeast Saccharomyces cerevisiae. Drug and chemical toxicology 2003, 26(2):75-85.
• 37. Zhao L, Si J, Wei Y, Li S, Jiang Y, Zhou R, Liu B, Zhang H: Toxicity of porcelain-fused-to-metal substrate to zebrafish (Danio rerio) embryos and larvae. Life sciences 2018, 203:66-71.
• 38. Choi J-W: Assessment of panoramic radiography as a national oral examination tool: review of the literature. Imaging science in dentistry 2011, 41(1):1-6.
• 39. Karagöz A, Beler M, Altun BD, Ünal İ, Cansız D, Gündüz H, Alturfan AA, Emekli-Alturfan E, Yalçınkaya ŞE: Panoramic dental X-ray exposure leads to oxidative stress, inflammation and apoptosis-mediated developmental defects in zebrafish embryos. Journal of Stomatology, Oral and Maxillofacial Surgery 2023, 124(6):101661.
• 40. Kollayan BY, et. al., Effects of Low-dose Ionizing Radiation on the Molecular Pathways Linking Nurogenesis and Autism Spectrum Disorders in Zebrafish Embryos. Drug and Chemical Toxicology, 2024. doi.org/10.1080/01480545.2024.2318444
• 41. Xu W, Zhou W, Wang H, Liang S: Roles of Porphyromonas gingivalis and its virulence factors in periodontitis. Advances in protein chemistry and structural biology 2020, 120:45-84.
• 42. Nibali L, Tatarakis N, Needleman I, Tu Y-K, D'Aiuto F, Rizzo M, Donos N: Association between metabolic syndrome and periodontitis: a systematic review and meta-analysis. The Journal of Clinical Endocrinology & Metabolism 2013, 98(3):913-920.
• 43. Gündüz G, Beler M, İsmail Ü, Cansiz D, ALTURFAN EE, Kose KN: Gingipain Injection Affects Intestinal OxidantAntioxidant Status and Alkaline Phosphatase in Overfed Zebrafish. Experimed 2023, 13(2):80-85.
• 44. Gündüz G, Beler M, Ünal İ, Cansız D, Emekli-Alturfan E, Kose KN: Endotoxin of Porphyromonas gingivalis amplifies the inflammatory response in hyperglycemia-induced zebrafish through a mechanism involving chitinase-like protein YKL-40 analogs. Toxicological Research 2023:1-12.