Research Article

Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

Volume: 32 Number: 3 December 28, 2018
  • Zeki Kara
  • Ali Sabır
  • Kevser Yazar
  • Osman Doğan
  • Mehmet Murat Şit
EN

Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage

Abstract

The present study was conducted to polyploidy possibility induction of grapes in the experiment, apical meristem was treated by colchicine. The factorial experiment based on randomized completely design with tree replications. The factors including different colchicine concentrations (0 as control, 1 gL-1, 2 gL-1, 3 gL-1, 4 gL-1, 5 gL-1, 6 gL-1) and four diploid grape genotypes cv. ‘Gök Üzüm’ (Vitis vinifera L.) which came from conventional ancient time and have a perfect adaptation to the territory, and cv. ‘Trakya İlkeren’ (Vitis vinifera L.) was bred in last two decades in Turkey, and ‘Isabella’ (Vitis labrusca L.) and 41 grape rootstock [Chasselas (Vitis vinifera L.) x (Vitis berlandieri Planch.)] were used. With this purpose eight colchicine doses were dropped to meristematic part of young plantlets twice a day (at 8.30 am and 18:00 pm) during 3 days when first true leaves emerge in greenhouse (25°C during day and 20°C at night). The morphological and cytological parameters were evaluated 16 weeks after the polyploidization treatments. Based on the size and shape, and density of stomata, the number of chloroplasts in guard cells, and chromosome counting in the root tip-end indicated that these descendants were diploids 2n=2x=38 like their parents that colchicine-induced mutation was failed in tested grape genotypes for tetraploid induction. On the other hand, only in thecv. ‘Trakya İlkeren’ an aneuploidy was observed by 5 gL-1 colchicine treated plantlet. Aneuploid ‘Trakya İlkeren’ plantlet was identified as 2n = 2x = 40. It is seeming that grape genotypes hardly response to colchicine induction for genome doubling. This is the first report for colchicine-induced aneuploid in grapes.

Keywords

Details

Primary Language

English

Subjects

Horticultural Production

Journal Section

Research Article

Authors

Zeki Kara This is me
Türkiye

Ali Sabır This is me
Türkiye

Osman Doğan This is me
Türkiye

Mehmet Murat Şit This is me
Türkiye

Publication Date

December 28, 2018

Submission Date

July 13, 2018

Acceptance Date

-

Published in Issue

Year 2018 Volume: 32 Number: 3

APA
Kara, Z., Sabır, A., Yazar, K., Doğan, O., & Şit, M. M. (2018). Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage. Selcuk Journal of Agriculture and Food Sciences, 32(3), 424-429. https://izlik.org/JA72SG43EZ
AMA
1.Kara Z, Sabır A, Yazar K, Doğan O, Şit MM. Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage. Selcuk J Agr Food Sci. 2018;32(3):424-429. https://izlik.org/JA72SG43EZ
Chicago
Kara, Zeki, Ali Sabır, Kevser Yazar, Osman Doğan, and Mehmet Murat Şit. 2018. “Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage”. Selcuk Journal of Agriculture and Food Sciences 32 (3): 424-29. https://izlik.org/JA72SG43EZ.
EndNote
Kara Z, Sabır A, Yazar K, Doğan O, Şit MM (December 1, 2018) Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage. Selcuk Journal of Agriculture and Food Sciences 32 3 424–429.
IEEE
[1]Z. Kara, A. Sabır, K. Yazar, O. Doğan, and M. M. Şit, “Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage”, Selcuk J Agr Food Sci, vol. 32, no. 3, pp. 424–429, Dec. 2018, [Online]. Available: https://izlik.org/JA72SG43EZ
ISNAD
Kara, Zeki - Sabır, Ali - Yazar, Kevser - Doğan, Osman - Şit, Mehmet Murat. “Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage”. Selcuk Journal of Agriculture and Food Sciences 32/3 (December 1, 2018): 424-429. https://izlik.org/JA72SG43EZ.
JAMA
1.Kara Z, Sabır A, Yazar K, Doğan O, Şit MM. Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage. Selcuk J Agr Food Sci. 2018;32:424–429.
MLA
Kara, Zeki, et al. “Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage”. Selcuk Journal of Agriculture and Food Sciences, vol. 32, no. 3, Dec. 2018, pp. 424-9, https://izlik.org/JA72SG43EZ.
Vancouver
1.Zeki Kara, Ali Sabır, Kevser Yazar, Osman Doğan, Mehmet Murat Şit. Effects of Colchicine Treatments on Some Grape Rootstock and Grape Varieties at Cotyledon Stage. Selcuk J Agr Food Sci [Internet]. 2018 Dec. 1;32(3):424-9. Available from: https://izlik.org/JA72SG43EZ

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