Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region

Dilek Kendi; Kubilay Kurtuluş Baştaş

EN TR

Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region

Abstract

Halo blight disease of bean caused by Pseudomonas savastanoi pv. phaseolicola, is a bacterial agent transmitted by seeds and causing serious damages. Seeds of dry bean were collected from 12 provinces in Central Anatolia Region and economically bean growing areas based on size the sown field. Isolation and identification of pathogen was based growth on Nutrient Agar(NA), Modified sucrose peptone (MSP), King’s B, levan production, growth at 37 °C, oxidase reaction, pectolytic activity, arginine dehidrolase, utilizing of carbon sources, arbutin and gelatin hydrolysis, nitrate reduction, H2S production, hypersensitive reaction on tobacco (cv. White Burley) tests. Typical leaf symptoms were obtained on bean cultivar Dermason which was inoculated by a suspension 108 cfu/ml of P. s. pv. phaseolicola in the pathogenicity tests. Molecular detection of the agent was made by Bio-PCR method and amplification of phaseolotoxin gen. According to obtained data, the pathogen was detectected on 38 seed samples from totally collected 175 dry bean samples and 21,71% of the seed samples was found contaminated by the pathogen in Central Anatolia Region.

Keywords

İç Anadolu Bölgesinde Fasulye Tohumlarında Pseudomonas savastanoi pv. phaseolicola Bulaşıklılığının Belirlenmesi

Abstract

Pseudomonas savastanoi pv. phaseolicola, fasulyelerde haleli yanıklık hastalığına sebep olan, tohumla taşınan ve ciddi zararlara yol açan bakteriyel etmendir. İç Anadolu Bölgesindeki 12 ilden ve ekonomik anlamda fasulye ekimi yapılan alanlardan, ekilen arazi büyüklüğü esas alınarak, tohum örnekleri toplanmıştır. Patojenin izolasyonu ve tanısında; Nutrient Agar (NA), Modified sucrose peptone (MSP), King B besi yerlerinde gelişim, levan oluşumu, 37 °C’de gelişim, oksidaz reaksiyonu, pektolitik aktivite testi, arginine dehidrolaz testi, karbon kaynaklarının kullanımı, arbutin ve jelati-nin hidrolizi, nitrat indirgenmesi, H2S üretimi, tütün yaprağında (cv. White Burley) aşırı duyarlılık reaksiyonu testleri esas alınmıştır. Patojenisite testlerinde, Dermason çeşidi fasulye bitkilerine yapılan 108 hücre/ml P. s. pv. phaseolicola süspansiyonu ile inokulasyon sonucu tipik yaprak lekeleri gözlenmiştir. Etmeninin moleküler tanısı, Bio-PCR yöntemi ve phaseolotoksin geninin amplifikasyonu ile tespit edilmiştir. Elde edilen bulgulara göre, toplam 175 kuru fasulye tohumu örneğinin 38’inde Pseudomonas savastanoi pv. phaseolicola tespit edilmiş ve İç Anadolu Bölgesinde etmenin fasulye tohumlarında bulaşıklılık oranı %21,71 olarak belirlenmiştir.

Keywords

Details

Primary Language

English

Subjects

Agricultural Engineering (Other)

Journal Section

Research Article

Authors

Dilek Kendi This is me
Türkiye

Kubilay Kurtuluş Baştaş This is me
Türkiye

Publication Date

June 25, 2011

Submission Date

January 1, 2011

Acceptance Date

-

Published in Issue

Year 2011 Volume: 25 Number: 2

IZ

https://izlik.org/JA37HF84WC

Cite

RIS / Bibtex

APA

Kendi, D., & Baştaş, K. K. (2011). Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region. Selcuk Journal of Agriculture and Food Sciences, 25(2), 8-16. https://izlik.org/JA37HF84WC

AMA

1.Kendi D, Baştaş KK. Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region. Selcuk J Agr Food Sci. 2011;25(2):8-16. https://izlik.org/JA37HF84WC

Chicago

Kendi, Dilek, and Kubilay Kurtuluş Baştaş. 2011. “Determination of Contamination of Pseudomonas Savastanoi Pv. Phaseolicola on Bean Seeds in Central Anatolia Region”. Selcuk Journal of Agriculture and Food Sciences 25 (2): 8-16. https://izlik.org/JA37HF84WC.

EndNote

Kendi D, Baştaş KK (June 1, 2011) Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region. Selcuk Journal of Agriculture and Food Sciences 25 2 8–16.

IEEE

[1]D. Kendi and K. K. Baştaş, “Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region”, Selcuk J Agr Food Sci, vol. 25, no. 2, pp. 8–16, June 2011, [Online]. Available: https://izlik.org/JA37HF84WC

ISNAD

Kendi, Dilek - Baştaş, Kubilay Kurtuluş. “Determination of Contamination of Pseudomonas Savastanoi Pv. Phaseolicola on Bean Seeds in Central Anatolia Region”. Selcuk Journal of Agriculture and Food Sciences 25/2 (June 1, 2011): 8-16. https://izlik.org/JA37HF84WC.

JAMA

1.Kendi D, Baştaş KK. Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region. Selcuk J Agr Food Sci. 2011;25:8–16.

MLA

Kendi, Dilek, and Kubilay Kurtuluş Baştaş. “Determination of Contamination of Pseudomonas Savastanoi Pv. Phaseolicola on Bean Seeds in Central Anatolia Region”. Selcuk Journal of Agriculture and Food Sciences, vol. 25, no. 2, June 2011, pp. 8-16, https://izlik.org/JA37HF84WC.

Vancouver

1.Dilek Kendi, Kubilay Kurtuluş Baştaş. Determination of Contamination of Pseudomonas savastanoi pv. phaseolicola on Bean Seeds in Central Anatolia Region. Selcuk J Agr Food Sci [Internet]. 2011 Jun. 1;25(2):8-16. Available from: https://izlik.org/JA37HF84WC