The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region)

Fulya Özdil; Mehmet Ali Yıldız

EN TR

The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region)

Abstract

In this study, the identification of Turkish honey bee populations was carried out using cytochrome C oxidase I and II intergenic region of mitochondrial genome. A total of 244 worker bees from 20 different locations in Turkey were used. Restriction fragment length polymorphism (RFLP) and DNA sequence data were utilized to identify the honeybee populations. DraI digestion of the COI-COII intergenic region generated four fragments. In this study, DraI digestion of the COI-COII intergenic region gave rise to a total of five DraI haplotypes which have been accurately identified from DNA sequence results. Three of the DraI haplotypes (47/41/64/420 bp-C1; 47/40/64/420 bp-C2 and 47/39/64/420 bp) have been reported in other papers but two of them (47/41/64/419 bp and 47/39/64/418 bp) were first seen in this study. DraI digestion of the COI-COII intergenic region generated four fragments in Turkish honeybees which previously have been found to be characteristic of the C lineage an these results confirmed that Turkish honey bee populations belong to East European and Mediterranean (C) lineage.

Keywords

Mitokondriyel DNA Sitokrom C Oksidaz I İle II Arasındaki İntergenik Bölge (COI-COII İntergenik Bölge) Bakımından Türkiye Bal Arısı Populasyonlarının Tanımlanması

Abstract

Bu araştırmada, Türkiye bal arısı populasyonları mitokondriyel genomda sitokrom C oksidaz I ile II arasındaki in-tergenik bölge bakımından tanımlanmıştır. Türkiye’nin 20 farklı yöresinden toplam 244 adet işçi arı örneği materyal olarak kullanılmış ve bal arısı populasyonlarının tanımlanmasında restriksiyon parça uzunluk polimorfizmi (RFLP) ve DNA dizi analizi yöntemlerinden yararlanılmıştır. COI-COII intergenik bölgenin DraI restriksiyon enzimi ile kesimi sonucu 4 banttan oluşan kesim modeli elde edilmiş ve Türkiye bal arılarında 5 haplotip belirlenmiştir. Bu haplotipler DNA dizi analizi sonuçları ile kesin bir şekilde belirlenmiştir. Bunlardan üçü (47/41/64/420 bç-C1; 47/40/64/420 bç-C2 ve 47/39/64/420 bç) daha önceki çalışmalarda bildirilmiş, diğer ikisi ise (47/41/64/419 bç ve 47/39/64/418 bç) ilk kez bu çalışma ile ortaya konmuştur. Çalışılan tüm örneklerde Dra restriksiyon enzimi ile kesim sonucu literatürde C genetik soyu için bildirilen ve 4 banttan oluşan kesim model elde edilmiş ve bu sonuçla Türkiye bal arısı populasyonlarının Doğu Avrupa ve Akdeniz (C) genetik soyu içerisinde yer aldığı belirlenmiştir.

Keywords

Details

Primary Language

English

Subjects

Zootechny (Other)

Journal Section

Research Article

Authors

Fulya Özdil This is me
Türkiye

Mehmet Ali Yıldız This is me
Türkiye

Publication Date

August 25, 2008

Submission Date

January 1, 2008

Acceptance Date

-

Published in Issue

Year 2008 Volume: 22 Number: 45

IZ

https://izlik.org/JA78RW35UE

Cite

RIS / Bibtex

APA

Özdil, F., & Yıldız, M. A. (2008). The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region). Selcuk Journal of Agriculture and Food Sciences, 22(45), 46-51. https://izlik.org/JA78RW35UE

AMA

1.Özdil F, Yıldız MA. The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region). Selcuk J Agr Food Sci. 2008;22(45):46-51. https://izlik.org/JA78RW35UE

Chicago

Özdil, Fulya, and Mehmet Ali Yıldız. 2008. “The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region)”. Selcuk Journal of Agriculture and Food Sciences 22 (45): 46-51. https://izlik.org/JA78RW35UE.

EndNote

Özdil F, Yıldız MA (August 1, 2008) The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region). Selcuk Journal of Agriculture and Food Sciences 22 45 46–51.

IEEE

[1]F. Özdil and M. A. Yıldız, “The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region)”, Selcuk J Agr Food Sci, vol. 22, no. 45, pp. 46–51, Aug. 2008, [Online]. Available: https://izlik.org/JA78RW35UE

ISNAD

Özdil, Fulya - Yıldız, Mehmet Ali. “The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region)”. Selcuk Journal of Agriculture and Food Sciences 22/45 (August 1, 2008): 46-51. https://izlik.org/JA78RW35UE.

JAMA

1.Özdil F, Yıldız MA. The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region). Selcuk J Agr Food Sci. 2008;22:46–51.

MLA

Özdil, Fulya, and Mehmet Ali Yıldız. “The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region)”. Selcuk Journal of Agriculture and Food Sciences, vol. 22, no. 45, Aug. 2008, pp. 46-51, https://izlik.org/JA78RW35UE.

Vancouver

1.Fulya Özdil, Mehmet Ali Yıldız. The Identification of Turkish Honey Bee Populations Inferred From Mitochondrial DNA Cytochrome C Oxidase I and Cytochrome C Oxidase II Intergenic Region (COI-COII Intergenic Region). Selcuk J Agr Food Sci [Internet]. 2008 Aug. 1;22(45):46-51. Available from: https://izlik.org/JA78RW35UE