Determination of CAST/MspI Polymorphism in Cattle by PCR-RFLP Method

Abstract

The calpastatin (CAST) gene is one of the genes associated with meat quality. Studies have shown that this gene is associated with quality traits such as body weight gain, carcass yield, meat tenderness and fat content in meat. In this study, the polymorphism of the CAST gene was determined using the PCR-RFLP method in Holstein, Simmental and Brown Swiss cattle. As a result of genotyping, three genotypes (MM, MN and NN) and two alleles (M and N) were determined. It was found that the MM genotype and the M allele had the highest frequency in all breeds examined, while the NN genotype had the lowest frequency. The NN genotype was only found in the Brown Swiss breed. All Simmental animals used in the study belonged to the MM genotype.

Keywords

• Calpastatin
• Cattle
• PCR_RFLP
• Holstein
• Simmental
• Brown Swiss

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