Obtaining Haploid Plants Via Anther Culture in Some Eggplant Varieties

Abstract

In plant breeding studies, anther culture is used to breed hybrid varieties, to transform tetraplaid plants that can be obtained through interspecific somatic hybridization into fertile dihaploid plants, and in recent years, to obtain diploid in protoplast fusion of haploid embryogenic calli obtained from anther culture. The frequency of obtaining haploid plants in anther culture depends on many factors such as the variety, the season in which it was taken and the use of suitable media for regeneration. In this study, it was aimed to determine the appropriate regeneration environment in the anther culture of Aydın Siyahı and Kemer eggplant varieties, which are widely grown in our country. In the anther culture study, C (callus), R (regeneration) and V3 media recommended by Dumas de Valux et al. (1982) were used as nutrient media. However, as C (callus) medium, Dumas de Vaulx et al. (1982) 3 different doses of 2,4 D, Kinetin and sucrose of the C medium reported were tested. The highest plant yield (36.4%) of the Aydin Siyahı variety was obtained from the modified Dumas de Vaulx et al. (1982), from 1 mg/l 2.4-D +1 mg/l Kinetin + 120 g/l sucrose medium, the highest plant yield (33.8%) of the Kemer variety was reported by Dumas de Vaulx et al. (1982), obtained from 5 mg/l 2.4-D + 5 mg/l Kinetin + 120 g/l sucrose medium. Of the 79 plants obtained from anther culture and whose ploidy level was examined, 60 were determined as haploid, 13 as diploid, 4 as triploid, 1 as tetraploid and 1 as mixoploid.

Keywords

• Solanum melongena
• anther culture
• haphoid plant

Supporting Institution

Çukurova Üniversitesi Bilimsel Araştırma Projeleri birimi tarafından desteklenmiştir.

Project Number

FBA-2015-3552

References

1. Alpsoy HC, Şeniz V (2007). Researches on the in vitro Androgenesis and Obtaining Haploid Plants in Some Eggplant Genotypes. Acta Horticulturae 729: 137–141.
2. Bașay S, Șeniz V, Ellialtıoğlu ȘŞ (2011). Obtaining dihaploid lines by using anther culture in the different eggplant cultivars. Journal of Food, Agriculture & Environment 9: 188-190.
3. Başay S, Ellialtıoğlu ŞŞ, Şeniz V (2010). Haploid embryo formation via anther culture in local and foreign eggplant varieties. VIII. Vegetable Agriculture Symposium, 23-26 June 2010, Van, Turkey. pp.:588-590.
4. Calabuig-Serna A, Porcel R, Corral-Martínez P, SeguíSimarro JM (2020). Anther culture in eggplant (Solanum melongena L.). In: Plant Embryogenesis. Methods in Molecular Biology, Volume 2122, Humana Press, New York, 283293.
5. Dumas De Vaulx R, and Chambonnet D (1982). Stimulation of plant production in eggplant (Solanum melongena L.) anther culture by treatment at +35°C and low growth substance concentrations. Agronomie 2 (10): 983-988.
6. Ellialtıoğlu ŞŞ, Başay S, Kuşvuran Ş (2012). Investigation of the relationship between pollen dimorphism and anther culture in eggplant. Journal of Agricultural Sciences Research 5(1): 149-152.
7. FAO (2024). Production and trade statistics. https://www.fao.org/faostat/en/#data/QCL (access date: 11.11.2024).
8. Gebeloğlu N, Ellialtıoğlu ŞŞ (2022). Eggplant breeding. Vegetable breeding, vol. III. Solanaceae, Gece publishing, Türkiye, pp. 319-412.

9. Kashyap V, Vinod Kumar S, Collonnier C, Fusari F, Haicour R, Rotino GL, Sihachakr D, Rajam MV (2003). Biotechnology of Eggplant. Scientia Horticulturae 97(1): 1-25.
10. Khatun F, Meah MB, Nasiruddin KM (2006). Regeneration of eggplant through anther culture. Pakistan Journal of Biological Sciences 9: 48–53.
11. Kumar S, Singh M, Prabhavathi K, Mathews A, Kumar S, Singh M, Mathews A (2003). In vitro induction of haploid in eggplant (Solanum melongena L.). Capsicum and Eggplant Newsletter 22: 147–150.
12. Mir R, Calabuig-Serna A, Seguí-Simarro JM (2021). Doubled haploids in eggplant. Biology 10(7): 685.
13. Salas P, Prohens J, Seguí-Simarro JM (2011). Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182: 261–274.
14. Salas P, Rivas-Sendra A, Prohens J, Seguí-Simarro JM (2012). Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184(2): 235–250.
15. Seguí-Simarro JM (2016). Androgenesis in Solanaceae (M. Germana, and M. Lambardi editors). In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY, 206–244.
16. Tuna M (2014). 2nd workshop on flow cytometry and its use in agricultural research. Namık Kemal University Faculty of Agriculture, Department of Field Crops,16-17 January 2014, Tekirdağ.
17. Vural GE, Ari E, Zengin S, Ellialtioglu SS (2019). Development of androgenesis studies on eggplant (Solanum melongena L.) in Turkey from past to present. In: Sustainable Crop Production, IntechOpen, London, 67–92.