Antioxidant Capacity and Quality Parameters of Early Maturing Soybean Genotypes Under Sivas Ecological Conditions

Abstract

Soybean (Glycine max L.) is a versatile crop characterized by its cholesterol- and saturated fat-free composition and high-quality protein content. Evaluating parameters such as antioxidant capacity, moisture, and ash content is crucial for assessing the nutritional quality of soybeans, which are widely consumed both globally and in Türkiye. Antioxidants are compounds that mitigate or neutralize the harmful effects of free radicals in the body. Dietary natural antioxidants are among the most critical factors for enhancing the body's antioxidant defense system. This study aimed to evaluate the antioxidant capacity, moisture, and ash content of early-maturing soybean genotypes cultivated under the Sivas ecological conditions. The highest antioxidant activity using the ABTS method was observed in the ÜNV-2 genotype (11.82 μmol TE/g dw), while the DPPH method revealed the ÜNV-15 genotype as the highest (4.03 μmol TE/g dw). The moisture content of the soybean genotypes and varieties used in this study ranged from 8.75% to 12.34%, while the ash content varied between 2.86% and 4.05%. Differences in all investigated traits among the samples were statistically significant at the 1% level. As a result, the ÜNV-2 and ÜNV-15 genotypes were prioritized due to their relatively higher antioxidant activity, and the Ataem-7 variety was preferred for its lower moisture content.

Keywords

• Glycine max L.
• DPPH
• ABTS
• quality traits

References

1. Akkuş İ (1995). Free Radicals and Their Pathophysiological Effects. Mimoza Publications, Konya.
2. Arbos KA, Claro LM, Borges L, Santos C, Weffort-Santos AM (2008). Human erythrocytes as a system for evaluating the antioxidant capacity of vegetable extracts. Nutrition Research, 28(7): 457-463.
3. Bayram Y, Torlak Y, Sağdıç O (2019). Antioxidant activity of rowan fruit. European Journal of Science and Technology, (16): 933-939.
4. Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617): 1199-1200.
5. Çilesiz Y, Nadeem MA, Gürsoy N, Kul R, Karaköy T (2023). Assessing the cooking and quality traits diversity in the seeds of faba bean germplasm. Turkish Journal of Agriculture and Forestry, 47(4): 448-466.
6. Erekul O (2020). International Innovation Cooperation Network for Increasing Soybean Production Due to Global Change (INNISOY), Aydın.
7. Güler D, Emeksiz F (2014). Soybean production, consumption and marketing in Türkiye. Master Thesis, Çukurova University(Unpublished), Türkiye.
8. Juliana C, Lister INE, Girsang E, Nasution AN, Widowati W (2020). Antioxidant and elastase inhibitor from black soybean (Glycine max L.) and its compound (daidzein). Journal of Biomedicine and Translational Research, 6(1): 11-14.

9. Kahkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47(10): 3954-3962.
10. Karaköy T, Erdem H, Baloch FS, Toklu F, Eker S, Kilian B, Özkan H (2012). Diversity of macro‐and micronutrients in the seeds of lentil landraces. The Scientific World Journal, (1): 710412.
11. Kaur C, Kapoor HC (2001). Antioxidants in fruits and vegetables–the millennium's health. International Journal of Food Science and Technology, 36(7): 703-725.
12. Kılınç K, Kılınç A (2002). Oxygen radicals as mediators of oxygen toxicity. Hacettepe Medical Journal, 33(2): 110-118.
13. Kolay B (2007). Effects of different tillage methods on yield and some soil properties in second crop soybean cultivation in Diyarbakır conditions. PhD Thesis, Harran University (Unpublished), Türkiye.
14. Osman AM, Wong KK, Hill SJ, Fernyhough A (2006). Isolation and the characterization of the degradation products of the mediator ABTS derived radicals formed upon reaction with polyphenols. Biochemical and Biophysical Research Communications, 340(2): 597-603.
15. Öztürk YE, Gülümser E, Mut H, Başaran U, Doğrusöz MÇ (2020). Determination of silage quality of hop mixtures with corn and feed soybean. Harran Journal of Agricultural and Food Sciences, 24(4): 440-446.
16. Pejuhan J (2018). Effects of foliar iron and zinc application with biological fertilizers on hay yield and yield components in forage soybean. PhD Thesis, Atatürk University, University of Science, Erzurum.
17. Prior RL, Cao G (2000). Analysis of botanicals and dietary supplements for antioxidant capacity. Journal of AOAC International, 83(4): 950-956.
18. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10): 1231-1237.
19. Reis FS, Heleno SA, Barros L, Sousa MJ, Martins A, Santos-buelga C, Ferreira I (2011). Toward the antioxidant and chemical characterization of mycorrhizal mushrooms from northeastern Portugal. Journal of Food Science, 76(6): 824–830.
20. Seeram NP, Zhang Y, Henning SM, Lee R, Niu Y, Lin G, Heber D (2006). Pistachio skin phenolics are destroyed by bleaching resulting in reduced antioxidative capacities. Journal of Agricultural and Food Chemistry, 54: 7036-7040.
21. Soedarjo M, Suhartina S, Nugrahaeni N, Wijanarko A, Putri DA, Fatmawati S (2020). The antioxidant activities and phenolic content of improved soybean seeds varieties of different grain sizes. IPTEK The Journal for Technology and Science, 31(1): 83-90.
22. Soretire AA, Olayinka A (2013). Response of soybean (Glycine max L.) to cow dung and wood ash application in tropical acid soils of South-Western Nigeria. Nigerian Journal of Soil Science, 23(2): 103-113.
23. TSE (2012). TS EN ISO 712, Grain and grain products - Determination of moisture content. Turkish Standard, Turkish Standards Institute, Ankara.
24. Wong SP, Leong LP, Koh JHW (2006). Antioxidant activities of aqueous extracts of selected plants. Food Chemistry, 99(4): 775-783.
25. Young IS, Woodside JV (2001). Antioxidants in health and disease. Journal of Clinical Pathology, 54(3): 176-186.
26. Yu J, Ahmedna M, Goktepe I, Dai J (2006). Peanut skin procyanidins: composition and antioxidant activities as affected by processing. Journal of Food Composition and Analysis, 19: 364-371.
27. Zaini NS, Karim R, Razis AFA, Zunairah W, Ibadullah W, Zawawi N (2024). Comparative analysis of ultra-high performance liquid chromatography-mass spectrometry and antioxidant properties between kenaf (Hibiscus cannabinus L.) seed and soybean (Glycine max) milk substitutes. In The 7th International Conference for Women in Science Without Borders, 2 April, Malaysia, p. 61.