Domates Kök ve Kökboğazı Çürüklüğü Hastalığının Kontrolünde Düşük Abiotik Stres ve DL-β-Aminobütirik Asit ile Asibenzolar-S-metil'in Priming Uygulamasında Karşılaştırılması

Abstract

Fusarium oxysporum f.sp. radicis-lycopersici (FORL) tarafından neden olunan taç ve kök çürüklüğü hastalığı, Türkiye'deki seralarda domates (Lycopersicon esculentum Mill) fidelerinde ve olgun bitkilerinde yıkıcı bir patojendir. Bilinen kimyasal savunma uyarıcılarından DL-β-Aminobütirik asit (BABA) ile abiyotik stresin (100 mM NaCl) sinerjik etkisi domates bitkilerinde test edilmiştir. Bitkilerin kökleri 125 ve 500 µg mL-1 BABA'ya daldırılmış veya bitkilerin yapraklarına ayrı ayrı BABA (125, 500 µg mL-1) püskürtülmüş ve ardından bitkiler, işlemden sonraki 1. günde spor süspansiyonu ile aşılanmıştır. Ayrıca, sadece BABA ve abiyotik stres (yaprak spreyi) ile yapılan başka bir çalışmada, bitkiler BABA (125 µg mL-1) çözeltisine daldırıldığında bitki hastalıklarına karşı kayda değer bir direnç gözlemlenmiştir. Bu kombinasyon, bitkiler üzerinde olumlu bir etki yaratmış ve bu etki, en yüksek BABA konsantrasyonu olan 500 µg mL-1'e daldırılan bitkilerle karşılaştırılabilir düzeyde olmuştur. Abiyotik stres ve BABA kombinasyonunun kullanıldığı bitkilerdeki hastalık şiddeti, kontrol grubuna ve sadece 500 µg mL-1 BABA'nın püskürtüldüğü (kontrol) bitkilere göre, aşılamadan sonraki 20. güne kadar daha düşük olmuştur. Bu nedenle, tuz stresi ve BABA'nın sinerjik etkisinin, bitkilerin FORL'ye karşı direncinde rol oynayabileceği öne sürülebilir.

Keywords

• Fusarium oxysporum f.sp. radicis-lycopersici
• Asibenzolar-S- metil
• DL-β-Aminobütirik Asit
• tuz stresi
• priming

Priming by Low Abiotic Stress and DL-β-Aminobutyric Acid Compared to Acibenzolar-S- methyl to Control of Tomato Crown and Root Rot Disease

Abstract

Crown and root rot disease caused by Fusarium oxysporum f.sp. radicis-lycopersici (FORL) is a destructive pathogen on the seedling and mature tomato (Lycopersicon esculentum Mill) plants in greenhouses of Turkey. The synergistic effect of abiotic stress (100 mM NaCl) by known chemical defense inducers DL-β-Aminobutryric acid (BABA) to FORL was tested on tomato plants. The roots of plants were immersed into 125, and 500 µg mL-1 BABA or foliages of plants were separately sprayed by BABA (125, 500 µg mL-1) before the plants were inoculated with fungal spore suspension by day 1 post treatment. Furthermore, in another study conducted on only by BABA and abiotic stress (foliar spray) resulted in remarkable plant disease resistance if the plants were detached into BABA (125 µg mL-1) solution. This combination caused a positive effect on plants, which was comparable with the plants detached into the highest BABA concentration at 500 µg mL-1. Disease severity of plants on which abiotic stress and BABA combination used was also lower than the control and BABA sprayed at 500 µg mL-1 to nontreated (control) plants alone until the 20th day post inoculation. Therefore, synergistic effect by salt stress and BABA can be suggested for plant resistance to FORL.

Keywords

• Fusarium oxysporum f.sp. radicis-lycopersici
• Acibenzolar-S- methy
• DL-β-Aminobutyric Acid
• salt stress
• priming

References

1. Altamiranda, E. A. G., Andreu, A. B., Daleo, G. R., & Olivieri, F. P. (2008). Effect of β-aminobutyric acid (BABA) on protection against Phytophthora infestans throughout the potato crop cycle. Australasian Plant Pathology, 37(4), 421-427. https://doi.org/10.1071/AP08033
2. Andreu, A. B., Guevara, M. G., Wolski, E. A., Daleo, G. R., & Caldiz, D. O. (2006). Enhancement of natural disease resistance in potatoes by chemicals. Pest Management Science: formerly Pesticide Science, 62(2), 162-170. https://doi.org/10.1002/ps.1142
3. Barilli, E., Prats, E., & Rubiales, D. (2010). Benzothiadiazole and BABA improve resistance to Uromyces pisi (Pers.) Wint. in Pisum sativum L. with an enhancement of enzymatic activities and total phenolic content. European journal of plant pathology, 128(4), 483-493. https://doi.org/10.1007/s10658-010-9678-x
4. Baysal, Ö. (2015). Host resistance: SAR and ISR to plant pathogenic bacteria. In Rajesh Kannan V, Baştaş KK (Eds), Sustainable Approaches to Controlling Plant Pathogenic Bacteria. CRC Press, Boca Raton. USA, pp. 206-224. http://dx.doi.org/10.1201/b18892
5. Baysal, Ö., Gürsoy, Y. Z., Örnek, H., Çetinel, B., & Teixeira da Silva, J. A. (2007). Enhanced systemic resistance to bacterial speck disease caused by Pseudomonas syringae pv. tomato by dl‐β‐aminobutyric acid under salt stress. Physiologia Plantarum, 129(3), 493-506. https://doi.org/10.1111/j.1399-3054.2006.00818.x
6. Baysal, Ö., Gürsoy, Y. Z., Örnek, H., & Duru, A. (2005). Induction of oxidants in tomato leaves treated with DL-β-Amino butyric acid (BABA) and infected with Clavibacter michiganensis ssp. michiganensis. European Journal of Plant Pathology, 112(4), 361-369. https://doi.org/10.1007/s10658-005-6234-1
7. Baysal, Ö., Soylu, E. M., & Soylu, S. O. N. E. R. (2003). Induction of defence‐related enzymes and resistance by the plant activator acibenzolar‐S‐methyl in tomato seedlings against bacterial canker caused by Clavibacter michiganensis ssp. michiganensis. Plant pathology, 52(6), 747-753. https://doi.org/10.1111/j.1365-3059.2003.00936.x
8. Baysal, O., Turgut, C., & Mao, G. (2005). Acibenzolar-S-methyl induced resistance to Phytophthora capsici in pepper leaves. Biologia plantarum, 49(4), 599-604. https://doi.org/10.1007/s10535-005-0055-0

9. Bektas, Y., & Eulgem, T. (2015). Synthetic plant defense elicitors. Frontiers in plant science, 5, 804. https://doi.org/10.3389/fpls.2014.00804
10. Jutta Chamsai, J. C., Siegrist, J., & Buchenauer, H. (2004). Mode of action of the resistance-inducing 3-aminobutyric acid in tomato roots against Fusarium wilt.
11. Cohen, Y. (1994). 3-Aminobutyric acid induces systemic resistance against Peronospore tabacina. Physiological and Molecular Plant Pathology, 44(4), 273-288. https://doi.org/10.1016/S0885-5765(05)80030-X
12. Cohen, Y., Rubin, A. E., & Kilfin, G. (2010). Mechanisms of induced resistance in lettuce against Bremia lactucae by DL-β-amino-butyric acid (BABA). European Journal of Plant Pathology, 126(4), 553-573. https://doi.org/10.1007/s10658-009-9564-6
13. Cohen, Y., Rubin, A. E., & Vaknin, M. (2011). Post infection application of DL-3-amino-butyric acid (BABA) induces multiple forms of resistance against Bremia lactucae in lettuce. European Journal of Plant Pathology, 130(1), 13-27. https://doi.org/10.1007/s10658-010-9724-8
14. Cohen, Y. R. (2002). β-aminobutyric acid-induced resistance against plant pathogens. Plant disease, 86(5), 448-457. https://doi.org/10.1094/PDIS.2002.86.5.448
15. Conrath, U. (2011). Molecular aspects of defence priming. Trends in plant science, 16(10), 524-531. https://doi.org/10.1016/j.tplants.2011.06.004
16. Conrath, U., Beckers, G. J., Flors, V., García-Agustín, P., Jakab, G., Mauch, F., ... & Mauch-Mani, B. (2006). Priming: getting ready for battle. Molecular plant-microbe interactions, 19(10), 1062-1071. https://doi.org/10.1094/MPMI-19-1062
17. Conrath, U., Pieterse, C. M., & Mauch-Mani, B. (2002). Priming in plant–pathogen interactions. Trends in plant science, 7(5), 210-216. https://doi.org/10.1016/s1360-1385(02)02244-6
18. Dempsey, D. M. A., Shah, J., & Klessig, D. F. (1999). Salicylic acid and disease resistance in plants. Critical reviews in plant sciences, 18(4), 547-575. https://doi.org/10.1080/07352689991309397
19. Desa, U.N. (2025). The sustainable development goals report 2025. United Nations. https://unstats.un.org/sdgs/report/2025/The-Sustainable-Development-Goals-Report-2025.pdf (access date: 11.02.2026).
20. Devran, Z., & Baysal, Ö. (2018). Induction of resistance to Meloidogyne incognita by DL-Beta amino butyric acid under salt stress condition. Australasian Plant Disease Notes, 13(1), 20. https://doi.org/10.1007/s13314-018-0304-7
21. Durrant, W. E., & Dong, X. (2004). Systemic acquired resistance. Annu. Rev. Phytopathol., 42(1), 185-209. https://doi.org/10.1146/annurev.phyto.42.040803.140421
22. Hamiduzzaman, M. M., Jakab, G., Barnavon, L., Neuhaus, J. M., & Mauch-Mani, B. (2005). β-Aminobutyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling. Molecular Plant-Microbe Interactions, 18(8), 819-829. https://doi.org/10.1094/MPMI-18-0819
23. Hwang, B. K., Sunwoo, J. Y., Kim, Y. J., & Kim, B. S. (1997). Accumulation of β-1, 3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems toPhytophthora capsici. Physiological and Molecular Plant Pathology, 51(5), 305-322. https://doi.org/10.1006/pmpp.1997.0119
24. Jakab, G., Cottier, V., Toquin, V., Rigoli, G., Zimmerli, L., Métraux, J. P., & Mauch-Mani, B. (2001). β-Aminobutyric acid-induced resistance in plants. European Journal of plant pathology, 107(1), 29-37. https://doi.org/10.1023/A:1008730721037
25. Justyna, P. G., & Ewa, K. (2013). Induction of resistance against pathogens by β-aminobutyric acid. Acta Physiologiae Plantarum, 35(6), 1735-1748. https://doi.org/10.1007/s11738-013-1215-z
26. Marcucci, E., Aleandri, M. P., Chilosi, G., & Magro, P. (2010). Induced Resistance by β‐Aminobutyric Acid in Artichoke against White Mould Caused by Sclerotinia sclerotiorum. Journal of Phytopathology, 158(10), 659-667. https://doi.org/10.1111/j.1439-0434.2010.01677.x
27. Olivieri, F. P., Lobato, M. C., González Altamiranda, E., Daleo, G. R., Huarte, M., Guevara, M. G., & Andreu, A. B. (2009). BABA effects on the behaviour of potato cultivars infected by Phytophthora infestans and Fusarium solani. European Journal of Plant Pathology, 123(1), 47-56. https://doi.org/10.1007/s10658-008-9340-z
28. Šašek, V., Nováková, M., Dobrev, P. I., Valentová, O., & Burketová, L. (2012). β-aminobutyric acid protects Brassica napus plants from infection by Leptosphaeria maculans. Resistance induction or a direct antifungal effect?. European journal of plant pathology, 133(1), 279-289. https://doi.org/10.1007/s10658-011-9897-9
29. Siegrist, J., Orober, M., & Buchenauer, H. (2000). β-Aminobutyric acid-mediated enhancement of resistance in tobacco to tobacco mosaic virus depends on the accumulation of salicylic acid. Physiological and Molecular Plant Pathology, 56(3), 95-106. https://doi.org/10.1006/pmpp.1999.0255
30. Slaughter, A. R., Hamiduzzaman, M. M., Gindro, K., Neuhaus, J. M., & Mauch-Mani, B. (2008). Beta-aminobutyric acid-induced resistance in grapevine against downy mildew: involvement of pterostilbene. European journal of plant pathology, 122(1), 185-195. https://doi.org/10.1007/s10658-008-9285-2
31. Thakur, M., & Sohal, B. S. (2013). Role of elicitors in inducing resistance in plants against pathogen infection: a review. International Scholarly Research Notices, 2013(1), 762412. https://doi.org/10.1155/2013/762412
32. Ton, J., Jakab, G., Toquin, V., Flors, V., Iavicoli, A., Maeder, M. N., ... & Mauch-Mani, B. (2005). Dissecting the β-aminobutyric acid–induced priming phenomenon in Arabidopsis. The Plant Cell, 17(3), 987-999. https://doi.org/10.1105/tpc.104.029728
33. Ton, J., & Mauch‐Mani, B. (2004). β‐amino‐butyric acid‐induced resistance against necrotrophic pathogens is based on ABA‐dependent priming for callose. The Plant Journal, 38(1), 119-130. https://doi.org/10.1111/j.1365-313X.2004.02028.x
34. Vakalounakis, D. J., & Fragkiadakis, G. A. (1999). Genetic diversity of Fusarium oxysporum isolates from cucumber: differentiation by pathogenicity, vegetative compatibility, and RAPD fingerprinting. Phytopathology, 89(2), 161-168. https://doi.org/10.1094/PHYTO.1999.89.2.161
35. Van Hulten, M., Pelser, M., Van Loon, L. C., Pieterse, C. M., & Ton, J. (2006). Costs and benefits of priming for defense in Arabidopsis. Proceedings of the National Academy of Sciences, 103(14), 5602-5607. https://doi.org/10.1073/pnas.0510213103
36. Walters, D. R., Ratsep, J., & Havis, N. D. (2013). Controlling crop diseases using induced resistance: challenges for the future. Journal of experimental botany, 64(5), 1263-1280. https://doi.org/10.1093/jxb/ert026
37. Wang, D., Amornsiripanitch, N., & Dong, X. (2006). A genomic approach to identify regulatory nodes in the transcriptional network of systemic acquired resistance in plants. PLoS pathogens, 2(11), e123. https://doi.org/10.1371/journal.ppat.0020123
38. Wu, C. C., Singh, P., Chen, M. C., & Zimmerli, L. (2010). L-Glutamine inhibits beta-aminobutyric acid-induced stress resistance and priming in Arabidopsis. Journal of experimental botany, 61(4), 995-1002. https://doi.org/10.1093/jxb/erp363
39. Yang, K. Y., Liu, Y., & Zhang, S. (2001). Activation of a mitogen-activated protein kinase pathway is involved in disease resistance in tobacco. Proceedings of the national academy of sciences, 98(2), 741-746. https://doi.org/10.1073/pnas.98.2.741
40. Zimmerli, L., Jakab, G., Métraux, J. P., & Mauch-Mani, B. (2000). Potentiation of pathogen-specific defense mechanisms in Arabidopsis by β-aminobutyric acid. Proceedings of the national academy of sciences, 97(23), 12920-12925. https://doi.org/10.1073/pnas.230416897