Comparison of Biochemical and Color parameters in Fresh Flower Stalks of Wild Rhubarb (Rheum ribes L.) from Diverse Locations

Abstract

This study aimed to evaluate the biochemical and color parameters of fresh flower stalks of Rheum ribes L. collected from four distinct locations in Eastern Anatolia, Türkiye. Significant variation was observed among genotypes in terms of organic acids, sugars, ascorbic acid, antioxidant capacity, and color traits. Succinic acid was the predominant organic acid, peaking in Bahçesaray (14718.47 μg g⁻¹ FW). Erçek accessions had the highest levels of fructose (1346.72 μg g⁻¹ FW), glucose (970.88 μg g⁻¹ FW), and total antioxidant capacity (73.97 μmol TE g⁻¹ FW). Muradiye samples contained the highest ascorbic acid (63.71 μg g⁻¹ FW), while Mount Erek showed maximum oxalic acid and chroma values. Color measurements also indicated greater vividness in Mount Erek and Muradiye accessions. Principal Component Analysis (PCA) explained 76.46% of the total variance in the first two components, effectively separating genotypes based on trait profiles. Cluster analysis confirmed these differences, grouping accessions by location with 100% bootstrap support. These results demonstrate the considerable biochemical and visual diversity of R. ribes populations across different environments, supporting their potential in food and nutraceutical applications.

Keywords

• Biochemical
• Color parameters
• Rheum ribes L.
• Wild rhubarb

Supporting Institution

Van Yuzuncu Yil University Scientific Research Projects (BAP) Coordination Unit

Project Number

2015-ZF-B081

Farklı Lokasyonlardan Toplanan Yabani Ravent (Rheum ribes L.) Bitkisinin Taze Çiçek Saplarında Biyokimyasal ve Renk Parametrelerinin Karşılaştırılması

Abstract

Bu çalışma, Türkiye'nin Doğu Anadolu bölgesindeki dört farklı lokasyondan toplanan Rheum ribes L. bitkisinin taze çiçek saplarının biyokimyasal ve renk parametrelerini değerlendirmeyi amaçlamıştır. Genotipler arasında organik asitler, şekerler, askorbik asit, antioksidan kapasitesi ve renk özellikleri açısından önemli farklılıklar gözlemlenmiştir. Süksinik asit, Bahçesaray'da en yüksek değere (14718,47 μg g⁻¹ FW) ulaşarak baskın organik asit olmuştur. Erçek örnekleri en yüksek fruktoz (1346,72 μg g⁻¹ FW), glikoz (970,88 μg g⁻¹ FW) ve toplam antioksidan kapasitesine (73,97 μmol TE g⁻¹ FW) sahip olmuştur. Muradiye örnekleri en yüksek askorbik asit (63,71 μg g⁻¹ FW) içerirken, Erek Dağı en yüksek oksalik asit ve kroma değerlerini göstermiştir. Renk ölçümleri ayrıca Erek Dağı ve Muradiye örneklerinde daha yüksek canlılık olduğunu gösterdi. Temel Bileşen Analizi (PCA), ilk iki bileşendeki toplam varyansın %76,46'sını açıklayarak, genotipleri özellik profillerine göre etkili bir şekilde ayırdı. Kümeleme analizi bu farklılıkları doğrulayarak, örnekleri %100 bootstrap desteğiyle konuma göre gruplandırdı. Bu sonuçlar, R. ribes popülasyonlarının farklı ortamlardaki önemli biyokimyasal ve görsel çeşitliliğini göstererek, gıda ve nutrasötik uygulamalarındaki potansiyellerini desteklemektedir.

Keywords

• Biyokimyasal
• Renk parametreleri,
• Rheum ribes L.
• Yabani ravent

Project Number

2015-ZF-B081

References

1. Abu-Irmaileh, B. E., & Afifi, F. U. (2003). Herbal medicine in Jordan with special emphasis on commonly used herbs. Journal of Ethnopharmacology 89: 193–197. https://doi.org/10.1016/S0378-8741(03)00283-6
2. Alkaya, D. B., Seyhan, S. A., & Ozturk, B. N. (2019). Influence of extraction method on antioxidant properties of Rheum ribes root extract. Ovidius University Annals of Chemistry 30(1): 44-47.
3. Andiç, S., Tunçtürk, Y., Ocak, E., & Köse, S. (2009). Some chemical characteristics of edible wild rhubarb species (Rheum ribes L.). Research Journal of Agriculture Biological Sciences 5(6): 973-977.
4. Aziz, N. M. (2013). Estimation Some of Metal Ions and Biological Constituents of Local Rheum Ribes (Rhubarb) of Kurdistan Region-IRAQ. Che Mater Res. 3(13): 27-31.
5. Baytop, T. (1999). Rheum ribes L. In T. Baytop (Ed.). Therapy with medicinal plants in Turkey. Istanbul. pp. 319–320.
6. Benzie, I. F. & Strain, J. J. (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry 239(1): 70-76.
7. Bevilacqua, A. E., & Califano AN (1989). Determination of organic acids in dairy products by high performance liquid chromatography. Journal of Food Science. 54(4): 1076-1076.
8. Cemeroglu, B. (2010). Gıda Analizleri. Gıda Teknolojisi Derneği Yayınları. Ankara. Türkiye. p.34.

9. Cemeroğlu, B., Yemenicioğlu, A., & Özkan, M. (2004). Meyve ve sebzelerin bileşimi. Meyve ve sebze işleme teknolojisi. Türkiye.
10. Ceylan, S., Cetin, S., Camadan, Y., Sara, O., & Ozsen, O. A. (2019). Tutus. Antibacterial and antioxidant activities of traditional medicinal plants from the Erzurum region of Turkey. Irish Journal of Medical Science 188(4): 1303-1309.
11. Chin, T. C., & Youngken, H. W. (1947). The Cytotaxonomy of Rheum. American Journal of Botany 34: 401-407.
12. Clapa, D., Borsai, O., Hârța, M., Bonta, V., Szabo, K., Coman, V., & Bobiș O (2020). Micropropagation. Genetic fidelity and phenolic compound production of Rheum rhabarbarum L. Plants 9(5): 656.
13. Cullen, J. (1967). Rheum L. in flora of Turkey and the East Aegean Islands. Vol. 2. Davis. P. H. (Ed.) (Edinburgh University Press 1967). Edinburgh. pp. 268-269.
14. Ekincialp, A., Erdinç, C., Turan, S., Cakmakci, Ö., Nadeem, M. A., Baloch, F. S., & Sensoy, S. (2019). Genetic characterization of Rheum ribes (wild rhubarb) genotypes in Lake Van basin of Turkey through ISSR and SSR markers. International Journal of Agriculture and Biology 21(4): 795-802.
15. Gundogdu, M., Muradoglu, F., Sensoy, R. G., & Yilmaz, H. (2011). Determination of fruit chemical properties of Morus nigra L.. Morus alba L. and Morus rubra L. by HPLC. Scientia Horticulturae 132: 37-41.
16. Jahan, N., Khalil-ur-Rahman, A. S., & Asi MR (2013). Phenolic acid and flavonol contents of gemmo-modified and native extracts of some indigenous medicinal plants. Pakistan Journal of Botany 45(5): 1515-1519.
17. Jang, H. D., Chang, K. S., Huang, Y. S., Hsu, C. L., Lee, S. H., & Su, M. (2007). Principal phenolic phytochemicals and antioxidant activities of three Chinese medicinal plants. Food Chemistry 103(3): 749-756.
18. Kalisz, S., Oszmiański, J., Kolniak-Ostek, J., Grobelna, A., Kieliszek, M., & Cendrowski, A. (2020). Effect of a variety of polyphenol compounds and antioxidant properties of rhubarb (Rheum rhabarbarum). Lwt 118: 108775. https://doi.org/10.1016/j.lwt.2019.108775.
19. Keser, S., Keser, F., Karatepe, M., Kaygili, O., Tekin, S., Turkoglu, I., & Sandal, S. (2020). Bioactive contents. In vitro antiradical, antimicrobial and cytotoxic properties of rhubarb (Rheum ribes L.) extracts. Natural Product Research 34(23): 3353-3357.
20. Kolodziejczyk-Czepas, J., & Liudvytska, O. (2021). Rheum rhaponticum and Rheum rhabarbarum: A review of phytochemistry, biological activities and therapeutic potential. Phytochemistry Review 20(3): 589-607.
21. Kosikowska, U., Smolarz, H., & Malm, A. (2010). Antimicrobial activity and total content of polyphenols of Rheum L. species growing in Poland. Central European Journal of Biology 5(6): 814-820.
22. Melgarejo, P., Salazar, D. M., & Artes, F. (2000). Organic acids and sugars composition of harvested pomegranate fruits. European Food Research Technology 211: 185-190.
23. Meral, R. (2017). The effect of different temperatures on antioxidant activity and phenolic profile of Rheum ribes. Yuzuncu Yil University Journal of Agricultural Sciences 27(1): 88-94.
24. Mezeyová, I., Mezey, J., & Andrejiová A (2021). The effect of the cultivar and harvest term on the yield and nutritional value of rhubarb juice. Plants 10(6): 1244.
25. Munzuroğlu, Ö., Karataş, F., & Gür, N. (2000). Işgın (Rheum ribes L.) bitkisindeki A. E ve C vitaminleri ile Selenyum Düzeylerinin Araştırılması. Turkish Journal of Biology 24(3): 397-404.
26. Neupane, P., & Lamichhane, J. (2020). Estimation of total phenolic content, total flavonoid content and antioxidant capacities of five medicinal plants from Nepal. Vegetos 33(2): 360-366.
27. Nizioł, J., Sekuła, J., & Ruman, T. (2017). Visualizing spatial distribution of small molecules in the rhubarb stalk (Rheum rhabarbarum) by surface-transfer mass spectrometry imaging. Phytochemistry 139: 72–80.
28. Oktay, M., Yildirim, A., Bilaloglu, V., & Gülçin, I. (2007). Antioxidant activity of different parts of Isgin (Rheum ribes L.). Asian Journal of Chemistry 19(4): 3047-3055.
29. Özdamar, K. (2004). Statistical Data Analysis with Package Programs (Multivariate Analysis). Kaan Kitapevi. Eskişehir. Türkiye.
30. Öztürk, M., Aydoğmuş, Öztürk, F., Duru, M. E., & Topçu, G. (2007). Antioxidant activity of stem and root extracts of rhubarb (Rheum ribes): An edible medicinal plant. Food Chemistry 103(2): 623-630.
31. Rodriguez-Delgado, M. A., Malovana, S., Perez, J. P., Borges, T., & Garcia-Montelongo, F. J. (2001). Separation pf phenolic compounds by high-performance liquid chromatography with absorbance and fluorimetric detection. Journal of Chromatography 912: 249-257.
32. Samancioglu, A., Sat, I. G., Yildirim, E., Ercisli, S., Jurikova, T., & Mlcek, J. (2016). Total phenolic and vitamin C content and antiradical activity evaluation of traditionally consumed wild edible vegetables from Turkey. Indian Journal of Traditional Knowledge 15 (2): 208-213.
33. Savran, H. S. (1999). Nar suyunda organik asit dağılımı. Master Thesis. Ankara University. Türkiye.
34. Seymen, M., Yavuz, D., Dursun, A., Kurtar, E. S., & Turkmen, O. (2019). Identification of drought-tolerant pumpkin (Cucurbita pepo L.) genotypes associated with certain fruit characteristics, seed yield, and quality. Agriccultural Water Management 221: 150–159. https://doi.org/10.1016/j.agwat.2019.05.009
35. Stoleru, V., Munteanu, N., Stan, T., Ipatioaie, C., Cojocaru, L., & Butnariu, M. (2019). Effects of production system on the content of organic acids in Bio rhubarb (Rheum rhabarbarum L.). Romanian Biotechnological Letters. 24(1): 184-192.
36. Tabata, M., Sezik, E., Honda, G., Yesilada, E., Fuki, H., Goto, K., & Ikeshiro, Y. (1994). Traditional medicine in Turkey III. Folk Medicine in East Anatolia. Van and Bitlis provinces. International Journal of Pharmacognosy 32(1): 3–12.
37. Tartik, M., Darendelioglu, E., Aykutoglu, G., & Baydas, G. (2015). The various biological activities of Rheum ribes extract on different types of cell. Turkish Journal of Nature and Science 4:1-7.
38. Turkmen, Ö., Cirka, M., & Sensoy, S. (2005). Initial evaluation of a new edible wild rhubarb species (Reum ribes L.) with a modified weighted scaling index method. Pakistan Journal of Biological Sciences 8(5): 763-765.
39. Tuzlacı, E., & Meriçli, A. H. (1992). Some studies on Işgın (Rheum ribes) and its distribution in Turkey. Proceedings of the IX. Symposium on Plant Drugs. Başer. K.C.H. (Ed). Publication of Anadolu University. No. 641. Eskişehir. Türkiye. pp. 336-341.
40. Vidal, N. P., Manful, C. F., Pham, T. H., Stewart, P., & Keough, D. (2020). The use of XLSTAT in conducting principal component analysis (PCA) when evaluating the relationships between sensory and quality attributes in grilled foods. MethodsX.. 7. 100835. https://doi.org/10.1016/j.mex.2020.100835.
41. Will, F., & Dietrich, H. (2013). Processing and chemical composition of rhubarb (Rheum rhabarbarum) juice. LWT-Food Science Technology 50(2): 673–678.
42. Zheng, Q. X., Wu, H. F., Jian, G. U. O., Nan, H. J., Chen, S. L., Yang, J. S., & Xu, X. D. (2013). Review of rhubarbs: chemistry and pharmacology. Chinese Herbal Medicines 5(1): 9-32.