Statik Manyetik Alanların Yem Bezelyesinin Kalitesi Üzerindeki Etkisi: Çok Boyutlu Bir Değerlendirme

Abstract

Bu çalışmada, yem bezelyesi (Pisum sativum L. subsp. arvense) tohumlarına uygulanan farklı yoğunluklardaki (0, 0.5, 0.7, 0.9 ve 1.1 Tesla) statik manyetik alanların fizyolojik parametreler, antioksidan savunma sistemleri ve yem kalitesi üzerine etkilerinin değerlendirilmesi amaçlanmıştır. Manyetik alan uygulamaları tohumlara 10 dakika süreyle uygulanmış ve ardından tohumlar kontrollü iklim koşullarında 28 gün boyunca yetiştirilmiştir. Klorofil ve karotenoid içeriği gibi fizyolojik parametrelere ek olarak, MDA, prolin, SOD, CAT ve APX gibi çeşitli biyokimyasal parametreler ile toplam fenolik bileşikler, flavonoidler, tanenler, DPPH radikal temizleme kapasitesi, ham protein içeriği, ADF, NDF ve besin bileşimi (K, Ca, Mg, P) değerlendirilmiştir. Sonuçlara göre, özellikle 0,7 T ve 0,9 T SMF uygulamaları klorofil b ve toplam klorofil içeriğinde artışlara yol açmış ve prolin ve MDA seviyeleri üzerinde düzenleyici etkilere sahip olmuştur. Aynı dozlarda, toplam fenolik içerik, flavonoid seviyeleri ve antioksidan enzim aktivitelerinde önemli artışlar gözlenmiştir. PCA ve ısı haritası analizleri, bu uygulamaların yem kalitesinin temel belirleyicilerini olumlu yönde etkilediğini desteklemektedir. Sonuç olarak, orta yoğunluklu SMF uygulamaları yem bezelyesi fidelerinde fizyolojik ve biyokimyasal süreçleri iyileştirmiş, sekonder metabolit üretimini teşvik etmiş ve genel yem kalitesini artırmıştır. Bu bağlamda, manyeto-priming, sürdürülebilir ve çevre dostu bir tarımsal iyileştirme yöntemi olarak ortaya çıkmaktadır.

Keywords

• yem bezelyesi
• antioksidan savunma
• fenolik bileşikler
• statik manyetik alan

Project Number

FGA-2024-1419.

The Impact of Static Magnetic Fields on the Quality of Forage Pea: A Multidimensional Assessment

Abstract

This study aimed to evaluate the effects of static magnetic fields of varying intensities (0, 0.5, 0.7, 0.9, and 1.1 Tesla) applied to forage pea (Pisum sativum ssp. arvense L.) seeds on physiological parameters, antioxidant defense systems, and forage quality. The magnetic field treatments were applied to the seeds for 10 minutes, after which the seeds were grown under controlled climate conditions for 28 days. In addition to physiological parameters such as chlorophyll and carotenoid content, various biochemical parameters including MDA, proline, SOD, CAT, and APX were assessed, along with total phenolic compounds, flavonoids, tannins, DPPH radical scavenging capacity, crude protein content, ADF, NDF, and nutrient composition (K, Ca, Mg, P). According to the results, especially the 0.7 T and 0.9 T SMFtreatments led to increases in chlorophyll b and total chlorophyll content and had regulatory effects on proline and MDA levels. At the same doses, significant increases were observed in total phenolic content, flavonoid levels, and antioxidant enzyme activities. PCA and heatmap analyses further supported that these treatments positively influenced key determinants of forage quality. In conclusion, moderate-intensity SMFtreatments improved physiological and biochemical processes in forage pea seedlings, promoted secondary metabolite production, and enhanced overall forage quality. In this context, magneto-priming emerges as a sustainable and environmentally friendly agricultural enhancement method.

Keywords

• forage pea
• antioxidant defense
• ; phenolic compounds
• static magnetic field

Supporting Institution

This research was funded by BAP on YOBU,

Project Number

FGA-2024-1419.

Thanks

We would like to thank the Yozgat Bozok University Scientific Research Projects Coordination Unit for supporting our work

References

1. Aebi, H. (1984). Catalase in vitro. Methods in Enzymology105:121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
2. Anand, A., Nagarajan, S., Verma, A. P. S., & Joshi, D. K. (2012). Enhancement of germination, growth and photosynthesis in maize (Zea mays) by exposure of seeds to static magnetic field. International Journal of Agriculture, Environment and Biotechnology5(1):27–34. https://doi.org/10.5958/j.2230-732X.5.1.004
3. Arvouet-Grand, A., Vennat, B., Pourrat, A., & Legret, P. (1994). Standardisation dun extrait de propoliset identification desprin cipaux constituants. Journal de pharmacie de Belgique49: 462-468.
4. Atak, C., Danilovi, V. I., & Rzakoulieva, A. (2003). Effect of magnetic field on the shoot regeneration of tobacco (Nicotiana tabacum L.). Biotechnology & Biotechnological Equipment17(2): 86–89.
5. Atak, C., Emiroğlu, Ö., Akliman, A. K., & Rzakoulieva, A. (2007). Effect of magnetic field on peroxidase activities of soybean tissue culture. Biologia Plantarum,47(3):437–440.
6. Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and soil39: 205-207.
7. Bate-Smith, E. C. (1975). Phytochemistry of proanthocyanidins. Phytochemistry14(4): 1107-1113.
8. Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry44(1): 276–287.

9. Brand-Williams, W., Cuvelier, M. E., Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology28(1): 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
10. Cakmak, T., Dumlupinar, R., & Erdal, S. (2010). Acceleration of germination and early growth of wheat and bean seedlings grown under various magnetic field and osmotic conditions. Bioelectromagnetics31(2):120–129.
11. Dardeniz, A., & Yalçınkaya, E. (2012). Effect of magnetic field on growth, chlorophyll content and antioxidant enzyme activities of grapevine (Vitis vinifera L.) seedlings. International Journal of Physical Sciences7(43): 5787–5796. https://doi.org/10.5897/IJPS12.012
12. De Souza, A., García, D, Sueiro, L., & Gilart, F. (2014). Improvement of the seed germination, growth and yield of onion plants by extremely low frequency non-uniform magnetic fields. Scientia Horticulturae176:63-69. Demmig-Adams, B., & Adams, W. W. (1996). The role of xanthophyll cycle carotenoids in the protection of photosynthesis. Trends in Plant Science1(1):21-26.
13. Doğrusöz, M. Ç., Mut, H., Başaran, U., & Gülümser, E. (2024). Enhancing drought resistance in grass pea: The positive regulatory role of plant-smoke solution. South African Journal of Botany166:483-491.
14. Esitken, A., & Turan, M. (2004). Alternating magnetic field effects on yield and plant nutrient element composition of strawberry. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science54(3):135–139. https://doi.org/10.1080/09064710310019939
15. Flórez, M., Carbonell, M. V., & Martínez, E. (2007). Exposure of maize seed tostationary magnetic fields: Effects on germination and early growth. Environmental and Experimental Botany59(1): 68-75.
16. Gong, H., Zhu, X., Chen, K., Wang, S., & Zhang, C. (2005). Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Science169: 313-321.
17. Harborne, J. B., & Williams, C. A. (2000). Advances in flavonoid research since 1992. Phytochemistry 55(6):481– 504.
18. Heath, R. L., & Packer, L. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics,125(1): 189–198.
19. Iqbal, M., Raja, N. I., & Mashwani, Z. U. R. (2012). Seed germination and seedling growth of Catharanthus roseus under the influence of magnetic fields. Pakistan Journal of Botany44(6):1851–1854.
20. Karkanis, A., Ntatsi, G., & Bilalis, D. (2016). Pea cultivation in Europe: From production to processing and consumption. Legume Perspectives13:23–26.
21. Kirk, J. T. O., & Allen, R. L. (1965). Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochemical and Biophsical Research Communications21(6): 523-530.
22. Kthiri, A., Hamimed, S., Tahri, W., Landoulsi, A., O’Sullivan, S., & Sheehan, D. (2024). Impact of silver ions and silver nanoparticles on biochemical parameters and antioxidant enzyme modulations in Saccharomyces cerevisiae under co-exposure to static magnetic field: a comparative investigation. International Microbiology27(4):953-966.
23. Mahajan, T. S., & Pandey, O. N. (2015). Influence of magnetic field on phenolic content and antioxidant activity of soybean. Biotechnology International8(1):35–43.
24. Maheshwari, B. L., & Grewal, H. S. (2009). Magnetic treatment of irrigation water: Its effects on vegetable crop yield and water productivity. Agricultural Water Management, 96(8):1229–1236.
25. Marschner, H. (1995). Mineral Nutrition of Higher Plants (2nd ed.). Academic Press.1:1258-1263.
26. Martínez, E., Carbonell, M. V., & Flórez, M. (2002). Magnetic biostimulation of initial growth stages of wheat (Triticum aestivum L.). Electromagnetic Biology and Medicine 21(1):43–53.
27. Mirza, A., & Doğrusöz, M. Ç. (2023). Yozgat koşullarında yem bezelyesi ve Macar fiği ile tritikale ikili karışımlarında ot kalitesinin belirlenmesi. ISPEC Journal of Agricultural Sciences, 7(1), 184-194.
28. Nakano, Y., & Asada, K. (1981). Hydrogen Peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology22 (5): 867-880.
29. Price, M. L., Van Scoyoc, S., & Butler, L. G. (1978). A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. Journal of Agricultural and Food Chemistry26(5):1214–1218.
30. Radhakrishnan, R., & Kumari, B. D. R. (2013). Pulsed magnetic field: A contemporary approach offers to enhance plant growth and yield of soybean. Plant Physiology and Biochemistry51:139–144. https://doi.org/10.1016/j.plaphy.2011.10.018
31. Shine, M. B., Guruprasad, K. N., & Anand, A. (2011). Enhancement of germination, growth, and photosynthesis in soybean by pretreatment of seeds with magnetic field. Bioelectromagnetics32(6):474–484. https://doi.org/10.1002/bem.20667
32. Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in enzymology Academic press299: 152-178.
33. Sipahioğlu, O., Hanife, M., Gülümser, E., Doğrusöz, M. Ç., & Başaran, U. (2022). Yem bezelyesi tarımında arpanın arkadaş bitki olarak kullanılması. ISPEC Journal of Agricultural Sciences, 6(2), 202-210.
34. Tkalec, M., Pavlica, M., Pevalek-Kozlina, B., & Vidaković-Cifrek, Ž. (2009). Effects of radiofrequency electromagnetic fields on seed germination and root meristematic cells of Allium cepa L. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 672(2):76–81. https://doi.org/10.1016/j.mrgentox.2008.09.022
35. Vashisth, A., & Nagarajan, S. (2010). Effect on germination and early growth characteristics in sunflower (Helianthus annuus) seeds exposed tostatic magnetic field. Journal of PlantPhysiology,167(2): 149-156.
36. Witham, F. H., Blaydesand, D. F., & Devlin, R. M. (1971). Experiments in plants physiology. New York: Van Nostrand Reinhold Cö.55-56.
37. Zhang, Y., Guo, H., Kwan, H., Wang, J. W., Kosek, J., & Bingwei, L. (2007). PAR-1 kinase phosphorylates Dlg and regulates its postsynaptic targeting at the Drosophila neuromuscular junction. Neuron53(2): 201-215.