Bazı Ekmeklik Buğday (Triticum aestivum L.) Genotiplerinin Kuraklığa Tepkisinin SSR Markörleri ile Belirlenmesi

Abstract

Bu çalışmada, 32 ekmeklik buğday genotipi, kuraklığa tepkileri açısından SSR markörleri kullanılarak değerlendirilmiştir. Tüm genotiplere on adet SSR primeri uygulanmış ve bunların yedisinin polimorfik olduğu belirlenmiştir. Toplam 25 polimorfik allel elde edilmiş, lokus başına allel sayısı 2 ile 6 arasında değişmiş ve SSR lokusu başına ortalama allel sayısı 3.6 olarak hesaplanmıştır. PIC değerleri 0.31 ile 0.97 arasında değişmiş ve ortalama 0.70 olarak bulunmuştur. En düşük ve en yüksek değerler sırasıyla Xbarc17 ve Xbarc12 primerlerinden elde edilmiştir. Genotipler arasındaki ikili genetik farklılık indeksleri minimum 0.083 ve maksimum 0.800 olarak belirlenmiştir. Bayraktar 2000 ve LR5 genotipleri, kuraklığa tolerant Gerek 79 çeşidine en yakın genotipler olurken; Sertak52 (C5) ile BL7 ve BL4 genotipleri ise kuraklığa tolerant Bayraktar 2000 çeşidine en yakın genotipler olarak belirlenmiştir. Sonuç olarak, bu çalışmada kullanılan genotiplerin ve SSR markörlerinin, gelecekte kuraklıkla ilgili ıslah çalışmaları ve genetik çalışmalar için ön verilerin oluşturulmasına yardımcı olacağı düşünülmektedir

Keywords

• ekmeklik buğday
• ıslah
• kuraklık SSR markörleri

Supporting Institution

Selçuk Üniversitesi Bilimsel Araştırmalar Koordinatörlüğü

Project Number

13201040

Ethical Statement

Gerek yok

Genetic Diversity of Some Bread Wheat (Triticum aestivum L.) Genotypes Using SSR Markers Associated with Drought

Abstract

This study aimed to assess the genetic diversity of 32 bread wheat genotypes using SSR markers associated with drought. In the study, 10 SSR primers were applied to all genotypes; BARC 024, WMC 9, and WMC 603 showed monomorphic patterns, while seven primers were polymorphic. In total, 25 polymorphic alleles were detected, with the number of alleles per locus ranging from 2 to 6 and a mean of 3.6 alleles per SSR locus. PIC (Polymorphic Information Content) values ranged from 0.31 to 0.97, with a mean value of 0.70. The lowest and highest values were obtained from Xbarc17 and Xbarc12, respectively. The dendrogram was constructed using UPGMA analysis, and the bread wheat genotypes were divided into three groups. Cluster I is further divided into two sub-clusters, Ia and Ib. Cluster II is further divided into two other sub-clusters, IIa and IIb. The pair-wise genetic dissimilarity indices revealed a minimum difference index of 0.083 and a maximum of 0.800 between genotypes. Bayraktar 2000 and LR5 and LR6 showed genetic similarity to the drought-tolerant cultivar Gerek 79, whereas BL8 and the drought-sensitive cultivar Bezostaja 1 (C10) showed the greatest genetic distance. Sertak52 (C5) and BL7 and BL4 showed genetic similarity to the drought-tolerant cultivar Bayraktar 2000, while BL6 and Bezostaja 1 (C10) showed the greatest genetic distance. BL1, BL3, and BL5 showed genetic similarity to the drought-sensitive cultivar Bezostaja 1, whereas LR1, LR2, LR5, and LR6 showed the greatest genetic distance. Overall, the genotypes and SSR markers used in this study provide preliminary data for future breeding and genetic studies related to drought-associated traits.

Keywords

• bread wheat
• breedin
• drought
• SSR markers

Supporting Institution

Selçuk University Scientific Research Projects Coordinatorship

Project Number

13201040

Ethical Statement

Not applicable

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