Etlik piliç embriyolarında in ovo sığır kolostrum tozu uygulamasının embriyonik yanıtları

Abstract

Bu çalışmada, etlik piliç embriyolarında kuluçkanın geç döneminde in ovo sığır kolostrum tozu (Cp) uygulamasına karşı embriyonik yanıtlar değerlendirilmiştir. Toplam 450 adet döllü Anadolu-T etlik piliç yumurtası enjeksiyon yapılmayan kontrol grubu (NC), fizyolojik tuz çözeltisi enjekte edilen kontrol grubu (PC) ve yumurta başına 1, 2, 3 veya 4 mg Cp olacak şekilde rastgele olarak altı gruba ayrılmıştır. İn ovo besleme kuluçkanın 18. gününde amniyotik sıvıya yapılmıştır. Embriyonik gelişim, kuluçkanın 18., 19. ve 20. günlerinde embriyo ağırlığı, yolk kesesi ağırlığı, gastrointestinal sistem (GIT) ağırlığı ve uzunluğu ile bazı organ ağırlıkları ölçülerek değerlendirilmiştir. Çıkışta ise civciv ağırlığı, rezidüel yolk kesesi ağırlığı ve kan biyokimyasal parametreleri belirlenmiştir. Kuluçkanın 19. gününde embriyo ağırlığı Cp1 grubunda kontrol gruplarından ve Cp4 grubunda PC grubundan daha yüksek bulunmuştur (p<0.05). Buna karşın, kuluçkanın 18. ve 20. günlerinde embriyo ağırlığı ile çıkıştaki civciv ağırlığında gruplar arasında fark belirlenmemiştir (p>0.05). Çıkışta rezidüel yolk kesesi ağırlığı Cp gruplarında NC grubuna göre daha düşük bulunmuştur (p<0.05). İn ovo Cp uygulamasının GIT gelişimi, metabolik organ ağırlıkları ve kuluçka randımanı üzerinde herhangi bir etkisi görülmemiştir (p>0.05). Cp4 grubunda toplam protein ve globulin düzeyleri artarken, üre düzeyi azalmıştır (p<0.05) ve diğer biyokimyasal parametreler etkilenmemiştir. Genel olarak sonuçlar, in ovo uygulanan sığır kolostrum tozunun etlik piliç embriyolarında kuluçkanın geç döneminde yolk besin maddelerinin kullanımını ve bazı biyokimyasal yanıtları modüle edebileceğini, ancak kuluçka randımanı veya organ gelişimini olumsuz etkilemediğini göstermektedir.

Keywords

• İn ovo besleme
• kuluçka
• sığır kolostrumu

Project Number

FLO-2024-3117

Effect of in Ovo Bovine Colostrum Powder Administration on Embryonic Growth, Yolk Sac Resorption, and Blood Biochemical Profiles in Anadolu-T Broiler Chickens

Abstract

This study evaluated embryonic responses to in ovo administration of bovine colostrum powder (Cp) during late incubation in broiler embryos. A total of 450 fertilized Anadolu-T broiler eggs were randomly allocated to six groups as a non-injected control (NC), a saline-injected control (PC), and groups receiving 1, 2, 3, or 4 mg of Cp per egg. Injections were performed into the amniotic cavity on day 18 of incubation. Embryonic development was assessed on days 18, 19, and 20 of incubation by measuring embryo weight, yolk sac weight, gastrointestinal tract (GIT) weight and length, and selected organ weights. At hatch, chick weight, residual yolk sac weight, and blood biochemical parameters were determined. On day 19, embryo weight was higher in the Cp1 group than in the control groups, and in the Cp4 group than in PC (p<0.05), whereas no differences were observed on days 18 or 20 of incubation or in chick weight at hatch (p>0.05). Residual yolk sac weight at hatch was lower in Cp groups than in the NC group (p<0.05). In ovo Cp administration did not affect GIT development, metabolic organ weights, or hatchability (p>0.05). Total protein and globulin levels increased, and urea level decreased in Cp4 (p<0.05), while other biochemical parameters were not affected. Overall, the results suggest that bovine colostrum powder administered in ovo may modulate yolk nutrient utilization and certain biochemical responses during late embryonic development without impairing hatchability or organ development in broiler embryos.

Keywords

• In ovo feeding
• Bovine colostrum
• Yolk sac utilization
• Embryonic development
• Serum biochemistry
• Poultry

Supporting Institution

Scientific Research Fund of Eskisehir Osmangazi University

Project Number

FLO-2024-3117

Ethical Statement

The study received approval from the local Ethics Committee for Experimental Animals (case number: HAYDEK-2024/995).

Thanks

This study was part of an undergraduate graduation thesis and was supported by the Scientific Research Fund of Eskisehir Osmangazi University (FLO-2024-3117). We thank the Scientific Research Fund and the Department of Animal Science, Faculty of Agriculture, for their support. The colostrum powder used was obtained within the scope of the “Yaşasın Buzağılar Gülsün Yüzler” Project (TR33/22/SOGEP/U1/0001 SOGEP) implemented by the Uşak Directorate of Provincial Agriculture and Forestry. The authors express sincere thanks to the Directorate, the project coordinator, and all staff involved for their valuable contributions.

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