Grain Yield and Quality Response of Common Bean Cultivar to Inoculation with Native Rhizobacteria

Abstract

A field experiment was carried out to investigate the effect of native Rhizobium and CIAT899 inoculation on grain yield, protein content, cookability, nonsoaker capacity, hidration coefficient, seed weight and total defects of dry bean (cv. Yunus 90, the most popular cultivar in Turkey). The number of live Rhizobium sp. in the inoculant was adjusted to 10⁸ CFU ml^-1 and the seed inoculation was performed at planting. Native Rhizobium inoculation significantly (P<0.01) increased the physical and cookability properties of the bean seed. Results also revealed that the inoculation with native Rhizobium increased the yield and protein content of beans compared to control (reference strain). Overall findings indicated that native Rhizobium inoculation, easy to handle, could be a promising fertilizer, improving the plant growth and seed quality. It is possible to conclude that the efficiency of native Rhizobium inoculation could further be improved with the additional use of biological, chemical or organic fertilizers. 

Keywords

• Dry Bean,Inoculation,Native Rhizobium,Physico-Chemical Properties

References

1. Abdelgani ME, Elsheikh EAE, and Mukhtar NO (1999). The effect of Rhizobium inoculation and chemical fertilization on seed quality of fenugreek seeds. Food Chemistry, 64: 289–293.
2. Abdelhamit M, Takatsugu H, and Oba S (2004). Composting of rice straw with oil seed rape cake and poultry manure and its effects on faba bean (Vicia faba L.) growth and soil properties. Bioresource Technology, 93: 183–189.
3. Ali AE, Salih FA, and Galiel A (1988). Effect of time of harvest on physical and chemical composition, cookability and yield of faba beans. FABIS Newsletter 20: 33-35.
4. Anonymous (1982). Methods of soil analysis (Editor, A.L. Page). Number 9, Part 2, Madison, Wisconsin, USA, 1159 pp
5. Anonymous (2000). Agricultural Structure (Production, Price, Value), TC Ministry State Institute of Statistics Publications, 2–6, Ankara, Turkey.
6. Anonymous (2012). Directorate of Konya Meteorological Data.
7. Babiker EE, Elsheikh EAE, Osman AG, and El Tinay AH, (1995). Effect of nitrogen fixation, nitrogen fertilization and viral infection on yield, tannin and protein contents and in vitro protein digestibility of faba bean. Plant Food Human Nutrition, 47: 257–263.
8. Bouyoucos Gd, (1951). A recalibration of the hydrometer method for making mechanical analysis of soil. Agronomy Journal, 43: 434–438.

9. Bremner JM, (1965). Methods of Soil Analysis Part 2. Chemical and Microbiological Properties. Ed. A.C.A. Black, American Society of Agronomy, Incorporated Publication Agronomy Series No: 9, Madison USA.
10. Cristou P, (1997). Biotechnology applied to grain legumes. Field Crops Research, 53: 83–97.
11. Cummings SP, Humphry DR, and Andrews MA (2001). A review of the current taxonomy and diversity of symbiotic rhizosphere and bulk soil nitrogen-fixing bacteria, which are beneficial to plants. In Aspects of Applied Biology 63 Plant Microbial Interactions: Positive interactions in relation to crop production and utilization, Wellesbourne, Warwick, UK. Association of Applied Biologists, 63: 5–18.
12. Daza A, Santamaria C, Rodriquez-Navarro DN, Camacho M, and Orive R, (2000). Perlite as a carrier for bacterial inoculants. Soil Biol. Biochemistry, 32: 567-572.
13. Duranti M, and Guis C, (1997). Legume seeds: protein content and nutritional value. Field Crops Research, 53: 31–45.
14. Düzgüneş O, Kesici T, and Gürbüz F, (1987). Statistic Methods II. Ankara, Turkey: University of Ankara Faculty of Agriculture, Public No: 1021.
15. Egli DB, (1998). Seed biology and the yield of grain crops. Wallingford, United Kingdom: Centre for Agriculture and Biosciences International.
16. El Tilib AMA, Ali AM, and Abdallah MA (1994). Effect of chicken manure and salinity on growth and leaf N, P and K content of okra grown on two soil types. University of Khartoum Journal of Agricultural Sciences, 2: 16-33.
17. Elmubarak AA, Salih FA, AbdelGalie A, and Ghorashi AM (1988). Effect of time of harvest on physical and chemical composition, cookability and yield of faba bean. FABIS Newsletter, 20: 33–36.
18. Elsheıkh EAE, and Osman AG (1995). Rhizobium leguminosarum inoculation and the decrease in damage of faba bean (Vicia faba) infected with broad bean mottle bromovirus and bean yellow mosaicpotyvirus. World Journal of Microbiology and Biotechnology, 11: 223–227.
19. Elsheıkh EAE, and Elzıdany AA (1997). Effects of Rhizobium inoculation, organic and chemical fertilizers on yield and physical properties of faba bean seeds. Plant Foods for Human Nutrition, 51: 137–144.
20. Elsheıkh EAE, and Elzıdany AA (1997). Effect of Rhizobium inoculation, organic and chemical fertilizers on proximate composition, in vitro protein digestibility, tannin and sulphur content of faba beans. Food Chemistry, 59: 41–45.
21. Elsheikh EAE, and Ibrahim KA (1999). The effect of Bradyrhizobium inoculation on yield and seed quality of guar (C. tetragonoloba L.). Food Chemistry,65: 183-187.
22. Graham PH (1981). Some problems of nodulation and symbiotic nitrogen fixation in Phaseolus vulgaris L. Field Crops Research, 4: 93–112.
23. Graham PH, Rosas JC, Estevez de Jensen C, Peralta E, Tlusty B, Acosta-Gallegos J, and Arraes Pereira PA (2003). Addressing edaphic constraints to bean production: The bean/cowpea CRSP project perspective. Field Crops Research, 82: 179–192.
24. Hızalan E, and Ünal H (1966). Soil chemical analysis. University of Ankara Agriculture Faculty Publications, 273, Ankara.
25. Jackson ML (1973). Soil Chemical Analysis. New Delhi: Prentice-Hall of India Private Ltd.
26. Khan MS, Zaidi A, and Amil M (1997). Associative effect of Bradyrhizobium sp. (Vigna) and phosphate solubilizing bacteria on mungbean [Vigna radiata (L.) Wilczek]. Biojournal, 9: 101–106.
27. Knudsen DG, Peterson A, and Pratt PF (1982). Lithium, sodium and potassium. In: Page AL, editor. Methods Soil Analysis, Part 2. Agronomy Monograph 9.Madison, WI, U.S.A., ASA and SSSA, p: 247-262.
28. Küçük Ç, and Kıvanç M (2008). The effect of Rhizobium sp. sp. inoculation on seed quality of bean in Turkey. Pakistan Journal of Biolog. Sci., 11(14): 1856–1859.
29. Martínez-Romero E (2003). Diversity of Rhizobium-Phaseolus vulgaris symbiosis: overview and perspectives. Plant Soil, 252: 11–23.
30. Mostasso L, Mostasso FL, Dias BG, Vargas MAT, and Hungria M (2002). Selection of bean (Phaseolus vulgaris L.) rhizobial strains for Brazilian Cerrados. Field Crops Research, 73: 121–132.
31. Olsen SR, and Sommers LE (1982). Phosphorus. In: Page AL, et al (eds), Methods of soil analysis, Part 2, 2nd edn, Agronomy Monograph, 9. ASA and ASSA, Madison WI, 403–430
32. Saini VK, Bhandari SC, and Tarafdar JC (2004). Comparison of crop yield, soil microbial C, N and P, N-fixation, nodulation and mycorrhizal infection in inoculated and non-inoculated sorghum and chickpea crops. Field Crop Research, 89: 39–47.
33. Salih FAA, and Elmubarak AA (1986). Effect of phosphorus application and time of harvest on the seed yield and quality of faba bean. FABIS Newsletter 15: 32–35.
34. Shisanya CA (2002). Improvement of drought adapted tepary bean (Phaseolus acutifolius A. Gray var. latifolius) yield through biological fixation in semi-arid SE-Kenya. European Journal of Agronomy, 16: 13-24.
35. Slattery JF, Pearce DJ, and Slattery WJ (2004). Effects of resident rhizobial communities and soil type on the effective nodulation of pulse legumes. Soil Biology and Biochemistry, 36: 1339–1346.
36. Soltanpour PN, and Workman SM (1981). Use of Inductively-Coupled Plasma Spectroscopy for the Simultaneous Determination of Macro and Micro Nutrients in NH4HCO3-DTPA Extracts of Soils. Developments in Atomic Plasma Analysis. In: Barnes R.M. (ed). USA., 673-680.
37. Sturz AV, Chritie B, and Nowak J (2000). Bacterial role in developing sustaineble systems of crop production. Critical Reviews in Plant Sciences, 19: 1-30.
38. Uyanöz R, Akbulut M, Çetin U, and Gültepe N (2007). Effects of Microbial Inoculation, Organic and Chemical Fertilizer on Yield and Physicochemical and Cookability Properties of Bean (P. vulgaris L.) Seeds Physicochemical Properties of Phaseolus vulgaris L. Seeds. The Philippine Agricultural Scientist, 90 (2): 168–172.
39. Vessey JK (2003). Plant growth-promoting rhizobacteria as biofertilizers. Plant Soil, 255: 571–586.
40. Williams P, and Nakkoul H (1983). Some new concepts of food legumes quality evaluation at ICARDA. Proceedings of the International Workshop on faba beans, kabuli chickpea, and lentils in the 198Os, eds M. C. Saxena and S. Varma. ICARDA, 16-20 May, Aleppo, Syria.