Investigation of Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) Polymorphism in Anatolian Black and Holstein Friesian Cattle Breeds

Year 2020, Volume: 34 Issue: 2, 137 - 140, 20.08.2020

Marwan Fadhıl İbrahim Aytekın Uğur Zülkadir

Abstract

Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene which is associated with development and growth in livestock. The present study aimed to investigate the polymorphisms of IGFBP-3 gene in Holstein Friesian (HF) and Anatolian Black (AB) cattle breeds. BsuRI (GG↓CC) restriction enzyme was used to detect of IGFBP-3 gene polymorphism. Although Anatolian Black breed was monomorphic (AA genotype), three genotypes (AA, AB and BB) were detected in Holstein Friesian breed by digestion of PCR products with BsuRI. The A and B allele frequencies were 0.57 and 0.43, respectively, in Holstein Friesian breed, while A allele frequency was1.00 in Anatolian Black breed. AA, AB and BB genotype frequencies were 0.32, 0.50 and 0.18, respectively in the Holstein Friesian breed. All three possible genotypes weare detected in Holstein Friesian breed. In the analysis made taking into account Hardy-Weingberg equilibrium, significant deviation was not observed in terms of genotype distributions (P>0.05). In other words, the Holstein Friesian cattle population was found in the Hardy-Weinberg equilibrium.

Keywords

Anatolian Black, Holstein Friesian, IGFBP-3, PCR-RFLP, BsuRI, Polymorphism