The Identification of Genetic Variation in Insulin-Like Growth Factors-I (IGF-I) Gene Region in Some Turkish Shee

Year 2020, Volume: 34 Issue: 3, 189 - 192, 25.12.2020

Mustafa Tawfeeq İsmail Keskin Fatma İlhan

Abstract

Insulin-like growth factors (IGFs) areknown as peptides with important metabolic effects required for cellular growth and metabolism. IGF-I is synthesized in liver tissue under the control of growth hormone (GH) and released to blood. In the process of GH to accelerate growth, IGF-I occupies a large place. IGF-I clearly showed its important effects on the growth of animal studies. In this study, promoter region of the IGF-I gene were amplified by polymerase chain reaction (PCR) in sheep breeds (Akkaraman, Kivircik, Awassi, Sakiz, Daglıc, Morkaraman, MalyaKarayaka (15 to 20 sheep per breed)) reared in Turkey. Informative restriction fragment length polymorphisms (RFLPs) were obtained with HaeII enzyme. The digestion of IGF-I gene with HaeII produced two alleles and three genotypes. Genotype frequencies were 59%, 19%and 22% for AA, BB and AB genotypes, respectively. Allele frequencies were 0.70 for A allele and 0.30 for the B allele. This study indicates the genetic profiles of the IGF-1 gene in native Turkish sheep breeds.

Keywords

Growth hormone, Insulin-like growth factor (IGF), Sheep, Polymerase chain reaction (PCR), Restriction fragment length polymorphism (RFL), Sheep breeds