Clonal Preselection in Grape (Vitis vinifera L.) Varieties of Ekşi Kara and Gök Üzüm

Year 2023, Volume: 37 Issue: 3, 444 - 456, 29.12.2023

Zeki Kara , Mehmet Hüsrev Öz Ali Sabır , Kevser Yazar , Osman Doğan , Ahmed Jalal Khaleel Khaleel Ali Imad Omar Omar Mehmet Naim Demirtaş , Serkan Önder , Nurdan Güngör , Mustafa Bağcı

Abstract

Ekşi Kara (functional female flowers) and Gök Üzüm (hermaphroditic flowers) are the two most important autochthonous varieties of middle Anatolia. This clone selection study started with mass-selection in producer vineyards consisting of approximately 5000 vines by The International Organization of Vine and Wine (OIV) clonal selection procedure. Two-years genetic and sanitation were examined visually in population and 220 clone candidates were ampelography and fertilization biology and bud fertility determined for Ekşi Kara variety. The clone candidates were ranked at the level of sums, with weighted grading of three-year yield, growth, and quality records. Sanitation analyses of the superior clones were made. 17 clones in the Ekşi Kara grape variety were selected according to their superior scores in genetic selection and sanitation analyses. Eleven clones were selected by mass selection from Gök Üzüm carried out in a single location, and 2 clones were selected with genetic selection scores and health tests. Grapevine fleck virus (GfKV) was the most common ( 29%) in the samples tested, Grapevine leafroll-associated virus 1+3 (GLRaV-1 + 3,  26%), Grapevine virus A (GVA, 12%), Grapevine leafroll associated virus-2 (GLRaV-2,  3%), Arabis mosaic virus (ArMV) / Grapevine fanleaf virus (GFLV) ( 1%) are fallowed with indicated percentage. Although virus and bacterial infections are common in the vineyards, enough healthy clones were selected. 17 Ekşi Kara and 2 Gök Üzüm clones selected as pollinators were grafted onto the 110R rootstock for clone comparison in homogeneous conditions, and a "Clone Comparison Vineyard" was established in Selçuk University.

Keywords

Clonal selection, clone comparison, native grape varieties, phytosanitary tests

References

• Abolmaaty A, Vu C, Oliver J, Levin R (2000). Development of a new lysis solution for releasing genomic DNA from bacterial cells for DNA amplification by polymerase chain reaction. Microbios 101(400):181-189.
• Akgül D, Savas N, Eskalen A (2014). First report of wood canker caused by Botryosphaeria dothidea, Diplodia seriata, Neofusicoccum parvum, andLasiodiplodia theobromae on grapevine in Turkey. Plant Disease 98(4):568-568. https://doi.org/10.1094/PDIS-07-13-0726-PDN.
• Alaniz S, León M, Vicent A, García-Jiménez J, Abad-Campos P, Armengol J (2007). Characterization of Cylindrocarpon species associated with black foot disease of grapevine in Spain. Plant disease 91(9):1187-1193. https://doi.org/10.1094/PDIS-91-9-1187.
• Aurand J (2017). OIV Process for the clonal selection of vines Resolution. OIV-VITI 564A-2017.
• Benlioğlu K, Özakman M (1998). Bağ üretim materyalinde kök uru etmeni Agrobacterium tumefaciens’in saptanması. Turkish Journal of Agriculture and Forestry 22:167-174.
• Carbonell-Bejerano P, Royo C, Mauri N, Ibáñez J, Zapater J (2019). Somatic variation and cultivar innovation in grapevine. In: Advances in Grape and Wine Biotechnology. IntechOpen pp 1-22. doi:10.5772/intechopen.86443.
• Cenis J (1992). Rapid extraction of fungal DNA for PCR amplification. Nucleic acids research 20(9):2380. doi: 10.1093/nar/20.9.2380.
• Cirami R, McCarthy M, Nicholas P (1993). Clonal selection and evaluation to improve production of Cabernet Sauvignon grapevines in South Australia. Australian Journal of Experimental Agriculture 33(2):213-220. https://doi.org/10.1071/EA9930213.
• Clark M, Adams A (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of general virology 34(3):475-483. https://doi.org/10.1099/0022-1317-34-3-475.
• Çelik H, Kunter B, Selli S, Keskin N, Akbaş B, Değirmenci K (2019). Kalecik Karası üzüm çeşidinde klon seleksiyonu ve seçilen klonlara ait ana damızlık parselinin oluşturulması. In: Kunter B, Keskin N (eds) Tarım Bilimlerinde Güncel Araştırma ve Değerlendirmeler. 1 edn. Ivpe, Cetinje-Montenegro, pp 59-95.
• Eibach R, Töpfer R (2015). Traditional grapevine breeding techniques. In: Grapevine Breeding Programs for the Wine Industry. Elsevier pp 3-22.
• Forneck A, Benja A, Rühl E (2009). Grapevine (Vitis ssp.): example of clonal reproduction in agricultural important plants. In: Lost Sex. Springer pp 581-598. doi: 10.1007/978-90-481-2770-2_27.
• Glass N, Donaldson G (1995). Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61(4):1323-1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995.
• Golino D, Fuchs M, Sim S, Farrar K, Martelli G (2017). Improvement of grapevine planting stock through sanitary selection and pathogen elimination. In: Grapevine Viruses: Molecular Biology, Diagnostics and Management. Springer pp 561-579. doi: 10.1007/978-3-319-57706-7_27.
• Gonçalves E, Martins A (2019). Methods for conservation of intra-varietal genetic variability in ancient grapevine varieties. In: BIO Web of Conferences, 2019. EDP Sciences, p 01029. doi: 10.1051/bioconf/20191501029.
• Halleen F, Schroers H, Groenewald J, Crous P (2004). Novel species of Cylindrocarpon (Neonectria) and Campylocarpon gen. nov. associated with black foot disease of grapevines (Vitis spp.). Studies in Mycology 50(2):431-455.
• Ibáñez J, Carreño J, Yuste J, Martínez-Zapater J (2015). Grapevine breeding and clonal selection programmes in Spain. In: Grapevine breeding programs for the wine industry. Elsevier pp 183-209.
• Jackson, R., 2008., Wine science: principles and applications. Academic press. Third Edition. London.
• Kara Z, Sabır A, Doğan O, Eker Ö (2016). Gök Üzüm (Vitis vinifera L.) çeşidinin ticari potansiyeli ve ampelografik özellikleri. Nevşehir Bilim ve Teknoloji Dergisi 5:395-410. https://doi.org/10.17100/nevbiltek.211038.
• Kara Z, Sabır A, Eker Ö, (2018). Ancient grape Vitis vinifera L. cv ‘Ekşi Kara’in Anatolia. Selçuk Journal of Agriculture and Food Sciences 32(3):416-423. doi: 10.15316/SJAFS.2018.116.
• Kara Z, Sabır A, Yazar K, Doğa, O, Khaleel A (2017a). Fertilization biology of ancient grape ‘Ekşi Kara’ (Vitis vinifera L.). Selçuk Journal of Agriculture and Food Sciences 31(2):92-97. doi: 10.15316/SJAFS.2017.25.
• Kara Z, Sabır A, Yazar K, Doğan O, Omar AİO (2017b). Fruitfulness of Ancient Grapevine Varety ‘Ekşi Kara’ (Vitis vinifera L.). Selçuk Journal of Agriculture and Food Sciences 31(3):62-68. https://doi.org/10.15316/SJAFS.2017.36.
• King E, Ward M, Raney D (1954). Two simple media for the demonstration of pyocyanin and fluorescin. Translational Research 44(2):301-307. doi: 10.5555/uri:pii:002221435490222X.
• Lemos A, Machado N, Egea-Cortines M, Barros A (2020). Assessment of quality parameters and phytochemical content of thirty ‘Tempranillo’grape clones for varietal improvement in two distinct sub-regions of Douro. Scientia Horticulturae 262:109096. doi: 10.1016/j.scienta.2019.109096.
• Loureiro M, Moreno-Sanz P, Suárez B (2011). Clonal preselection of grapevine cultivars of the appellation “Cangas Quality Wine” (Asturias, Spain). Horticultural Science 38(2):71-80. doi: 10.17221/87/2010-HORTSCI.
• Mannini F (1998). Clonal selection in grapevine: interactions between genetic and sanitary strategies to improve propagation material. In: VII International Symposium on Grapevine Genetics and Breeding 528 pp 703-712. doi: 10.17660/ActaHortic.2000.528.106.
• Mannini F, Digiaro M (2017). The effects of viruses and viral diseases on grapes and wine. In: Grapevine viruses: molecular biology, diagnostics, and management. Springer pp 453-482.
• Mannini F, Rolle L, Guidoni S (2002). Vineyard management to optimize grape quality in virus-free clones of Vitis vinifera L. In: VIII International Conference on Grape Genetics and Breeding 603 pp 121-126.
• Martínez J, Vicente T, Martínez T, Chavarri J, García-Escudero E (2006). Una nueva variedad blanca para la DO Ca. Rioja: el Tempranillo blanco. In: XIX Congreso Mundial de la Viña y el Vino (OIV) pp 41-43.
• Muganu M, Paolocci M, Primiceri S, Tartaglia R, Benucci I, Cerreti M, D'Onofrio C, Paolacci A, Bignami C (2019). Intravarietal variability of Romanesco variety (Vitis vinifera L.). In: BIO Web of Conferences. EDP Sciences, pp 01006.
• OIV (2009). The International Organization of Vine and Wine, OIV descriptor list for grape varieties and Vitis species 2nd ed. 178 pp. 18 rue d‟Aguesseau – 75008 Paris.
• OIV (2012). OIV general form for the description of vine varieties (Vitis spp.). http://www.oiv.int/public/medias/405/viti-2012-2-en.pdf (accessed 25.08.2022).
• Poyraz D, Onoğur E (2013). Studies on esca and Petri diseases in grapevine nurseries and vineyards in Aegean Region. The Journal of Turkish Phytopathology 42(1-2-3):13-27.
• Rajasekaran K, Mullins M (1979). Embryos and plantlets from cultured anthers of hybrid grapevines. Journal of Experimental Botany 30(3):399-407. https://doi.org/10.1093/jxb/30.3.399.
• Rizzo D, Materazzi A, Stefani L, Farina P, Vanarelli S, Panattoni A, Luvisi A (2015). Distribution of regulated viruses in cv. Sangiovese vineyards in Tuscany. Journal of Plant Pathology 97(2):131-135.
• Roach MJ, Johnson DL, Bohlmann J, van Vuuren HJ, Jones SJ, Pretorius IS, Schmidt SA, Borneman AR (2018). Population sequencing reveals clonal diversity and ancestral inbreeding in the grapevine cultivar Chardonnay. PLoS genetics 14(11):e1007807. https://doi.org/10.1371/journal.pgen.1007807.
• Roy M, Sasser M (1983). A medium selective for Agrobacterium tumefaciens biotype-3. Phytopathology 73:810.
• Rühl E, Konrad H, Lindner B, Bleser E (2003). Quality criteria and targets for clonal selection in grapevine. In: I International Symposium on Grapevine Growing, Commerce and Research 652 pp 29-33. doi: 10.17660/ActaHortic.2004.652.1.
• Šikuten I, Preiner D, Marković Z, Andabaka Ž, Stupić D, Štambuk P, Žulj Mihaljević M, Pejić I, Šimon S, Karoglan Kontić J (2018). Individual clonal selection of Croatian native cultivars. In: 22nd Int'l Geisenheim Conference on Grapevine Propagation Geisenheim, Njemačka.
• Stanimirović B, Djordjević J, Pejin B, Maletić R, Vujović D, Raičević P, Tešić Ž (2018). Impact of clonal selection on Cabernet Franc Grape and wine elemental profiles. Scientia Horticulturae 237:74-80. doi: 10.1016/j.scienta.2018.03.043.
• Stenkamp S, Becker M, Hill B, Blaich R, Forneck A (2009). Clonal variation and stability assay of chimeric Pinot Meunier (Vitis vinifera L.) and descending sports. Euphytica 165(1):197. doi: 10.1007/s10681-008-9807-1.
• Szegedi E, Bottka S (2002). Detection of Agrobacterium vitis by polymerase chain reaction in grapevine bleeding sap after isolation on a semiselective medium. Vitis-Journal of Grapevine Research 41(1):37-42.
• van Leeuwen C, Destrac-Irvine A, Dubernet M, Duchêne E, Gowdy M, Marguerit E, Pieri P, Parker A, de Rességuier L, Ollat N (2019). An update on the impact of climate change in viticulture and potential adaptations. Agronomy 9(9):514. https://doi.org/10.3390/agronomy9090514.
• Vončina D, Preiner D, Šimon S, Cvjetković B, Maletić E, Pejić I, Karoglan Kontić J (2019). Distribution of nine viruses in Croatian autochthonous grapevine (Vitis vinifera L.) cultivars from Dalmatian region included in clonal selection. Journal of Central European Agriculture 20(1):262-273. https://doi.org/10.5513/JCEA01/20.1.2008
• Vondras A, Minio A, Blanco-Ulate B, Figueroa-Balderas R, Penn M, Zhou Y, Seymour D, Ye Z, Liang D, Espinoza, L (2019). The genomic diversification of grapevine clones. BMC Genomics 20(1):972. https://doi.org/10.1186/s12864-019-6211-2.
• White T, Bruns T, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18(1):315-322.