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Optimizing Sodium Azide (NaN₃) Mutagenesis for Cumin (Cuminum cyminum L.) Improvement

Year 2024, Volume: 38 Issue: 3, 427 - 433, 16.12.2024

Abstract

Cumin (Cuminum cyminum), an anciently cultivated plant of the Apiaceae family, holds significance for its aromatic seeds widely used in culinary practices globally. Despite its culinary and medicinal value, cumin faces challenges in cultivation due to diseases, pests, and weed infestations, with Alternaria leaf blight and Fusarium wilt being notable threats. Mutation breeding, a favored technique among breeders, introduces genetic variation through chemical mutagens like sodium azide, enabling the development of cumin varieties resistant to herbicides and diseases while ensuring high yields. This study aims to optimize sodium azide application as a chemical mutagen to enhance cumin breeding programs, emphasizing the importance of dosage and treatment duration in achieving desired mutation efficiency. The experimental results demonstrate significant impacts of sodium azide on germination parameters, with an optimal treatment duration of 3 hours for 3 mM sodium azide. Further research is needed to determine the effects of other variables on mutagen action, as well as M1 plant survival and reproduction.

References

  • Agarwal U, Pathak DP, Kapoor G, Bhutani R, Roper R, Gupta V, Kant R (2017). Review on Cuminum cyminum–Nature's magical seeds. Journal of Chemical and Pharmaceutical Research 9(9): 180–187.
  • Agrawal L, Kumar M (2021). Improvement in ornamental, medicinal, and aromatic plants through induced mutation. Journal of Applied Biology and Biotechnology 9(4): 162-169.
  • Bharti R, Kumar S, Parekh, MJ (2018). Development of genomic simple sequence repeat (gSSR) markers in cumin and their application in diversity analyses and cross-transferability. Industrial Crops and Products 111: 158–164.
  • Budak İ, Serim AT, Asav Ü (2020). Ankara İli kimyon (Cuminum cyminum L.) tarlalarında bulunan yabancı otların tespiti. Turkish Journal of Weed Science 23(2): 137-143.,
  • Chaudhary L, Sharma R, Kumar M (2021). Estimation of LD50 and effect of sodium azide on germination and seedling parameters of different cultivars of Cajanus cajan (L.) Millspaugh. Toxicology and Environmental Health Sciences 13(3): 279-285.
  • Dhandapani S, Subramanian VR, Rajagopal S, Namasivayam N (2002). Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacological Research 46(3): 251-255.
  • Didwania N (2019). Diseases of cumin and its management. Diseases of Medicinal and Aromatic Plants and Their Management, Today and Tomorrow Printers and Publisher, New Delhi. India, pp. 339-35
  • Emrani SN, Arzani A, Saeidi G (2011). Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens. African Journal of Biotechnology 10(59): 12602-12613.
  • Ingle AT, Sable AD, Zote RK (2018). Studies on morphological and phytochemical variation between two varieties of fenugreek (Trigonella foenum-graecum l.) at different concentration of sodium azide. International Journal of Current Microbiology and Applied Sciences 7: 1655-1661.
  • Kumar S, Saxena SN, Mistry J, Fougat R, Solanki RK, Sharma R (2015). Understanding Cuminum cyminum: an important seed spice crop of arid and semi arid regions. International Journal of Seed Spices 5 (2): 1–19.
  • Kanani P, Shukla YM, Modi AR, Subhash N, Kumar S (2019). Standardization of an efficient protocol for isolation of RNA from Cuminum cyminum. Journal of King Saud University – Science 31(4): 1202–1207. Doi: https://doi.org/10.1016/j.jksus.2018.12.008.
  • Lal G, Mehta RS, Godara AS, Mahala HR, Maheria SP, Singh B (2014) Performance of cumin varieties and technological interventions at farmers fields in Jaisalmer district of Rajasthan. International Journal of Seed Spices 4(1): 9–13.
  • Lodha S, Mawar R (2014). Cumin wilt management – a review. J. Spices Aromatic Crops 23 (2): 145–155.
  • Mensah JK, Obadoni BO (2007). Effects of sodium azide on yield parameters of groundnut (Arachis hypogaea L.). African Journal of Biotechnology 6(6): 668-671.
  • Mensah JK, Obadoni BO, Akomeah PA, Ikhajiagbe B, Ajibolu. (2007). The effects of sodium azide and colchicine treatments on morphological and yield traits of sesame seed (Sesame indicum L.). African Journal of Biotechnology 6(5): 534-538.
  • Oladosu Y, Rafii MY, Abdullah N, Hussin G, Ramli A, Rahim HA, Miah G, Usman, M (2016). Principle and application of plant mutagenesis in crop improvement: a review. Biotechnology & Biotechnological Equipment 30(1): 1-16.
  • Prabha R, Dixit V, Chaudhary BR (2010). Comparative spectrum of sodium azide responsiveness in plants. American-Eurasian Journal of Agricultural & Environmental Sciences 8(6): 779-783.
  • Patel HN, Patel MP, Soni NV, Patel NB, Patel AM, Patel NI (2023). Effect of induced mutagenesis in M1 and M2 generations of cumin (Cuminum cyminum L.). Electronic Journal of Plant Breeding 14(2): 687-694.
Year 2024, Volume: 38 Issue: 3, 427 - 433, 16.12.2024

Abstract

References

  • Agarwal U, Pathak DP, Kapoor G, Bhutani R, Roper R, Gupta V, Kant R (2017). Review on Cuminum cyminum–Nature's magical seeds. Journal of Chemical and Pharmaceutical Research 9(9): 180–187.
  • Agrawal L, Kumar M (2021). Improvement in ornamental, medicinal, and aromatic plants through induced mutation. Journal of Applied Biology and Biotechnology 9(4): 162-169.
  • Bharti R, Kumar S, Parekh, MJ (2018). Development of genomic simple sequence repeat (gSSR) markers in cumin and their application in diversity analyses and cross-transferability. Industrial Crops and Products 111: 158–164.
  • Budak İ, Serim AT, Asav Ü (2020). Ankara İli kimyon (Cuminum cyminum L.) tarlalarında bulunan yabancı otların tespiti. Turkish Journal of Weed Science 23(2): 137-143.,
  • Chaudhary L, Sharma R, Kumar M (2021). Estimation of LD50 and effect of sodium azide on germination and seedling parameters of different cultivars of Cajanus cajan (L.) Millspaugh. Toxicology and Environmental Health Sciences 13(3): 279-285.
  • Dhandapani S, Subramanian VR, Rajagopal S, Namasivayam N (2002). Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacological Research 46(3): 251-255.
  • Didwania N (2019). Diseases of cumin and its management. Diseases of Medicinal and Aromatic Plants and Their Management, Today and Tomorrow Printers and Publisher, New Delhi. India, pp. 339-35
  • Emrani SN, Arzani A, Saeidi G (2011). Seed viability, germination and seedling growth of canola (Brassica napus L.) as influenced by chemical mutagens. African Journal of Biotechnology 10(59): 12602-12613.
  • Ingle AT, Sable AD, Zote RK (2018). Studies on morphological and phytochemical variation between two varieties of fenugreek (Trigonella foenum-graecum l.) at different concentration of sodium azide. International Journal of Current Microbiology and Applied Sciences 7: 1655-1661.
  • Kumar S, Saxena SN, Mistry J, Fougat R, Solanki RK, Sharma R (2015). Understanding Cuminum cyminum: an important seed spice crop of arid and semi arid regions. International Journal of Seed Spices 5 (2): 1–19.
  • Kanani P, Shukla YM, Modi AR, Subhash N, Kumar S (2019). Standardization of an efficient protocol for isolation of RNA from Cuminum cyminum. Journal of King Saud University – Science 31(4): 1202–1207. Doi: https://doi.org/10.1016/j.jksus.2018.12.008.
  • Lal G, Mehta RS, Godara AS, Mahala HR, Maheria SP, Singh B (2014) Performance of cumin varieties and technological interventions at farmers fields in Jaisalmer district of Rajasthan. International Journal of Seed Spices 4(1): 9–13.
  • Lodha S, Mawar R (2014). Cumin wilt management – a review. J. Spices Aromatic Crops 23 (2): 145–155.
  • Mensah JK, Obadoni BO (2007). Effects of sodium azide on yield parameters of groundnut (Arachis hypogaea L.). African Journal of Biotechnology 6(6): 668-671.
  • Mensah JK, Obadoni BO, Akomeah PA, Ikhajiagbe B, Ajibolu. (2007). The effects of sodium azide and colchicine treatments on morphological and yield traits of sesame seed (Sesame indicum L.). African Journal of Biotechnology 6(5): 534-538.
  • Oladosu Y, Rafii MY, Abdullah N, Hussin G, Ramli A, Rahim HA, Miah G, Usman, M (2016). Principle and application of plant mutagenesis in crop improvement: a review. Biotechnology & Biotechnological Equipment 30(1): 1-16.
  • Prabha R, Dixit V, Chaudhary BR (2010). Comparative spectrum of sodium azide responsiveness in plants. American-Eurasian Journal of Agricultural & Environmental Sciences 8(6): 779-783.
  • Patel HN, Patel MP, Soni NV, Patel NB, Patel AM, Patel NI (2023). Effect of induced mutagenesis in M1 and M2 generations of cumin (Cuminum cyminum L.). Electronic Journal of Plant Breeding 14(2): 687-694.
There are 18 citations in total.

Details

Primary Language English
Subjects Crop and Pasture Breeding
Journal Section Research Article
Authors

Furkan Çoban 0000-0003-2815-8988

Early Pub Date December 13, 2024
Publication Date December 16, 2024
Submission Date May 23, 2024
Acceptance Date September 29, 2024
Published in Issue Year 2024 Volume: 38 Issue: 3

Cite

EndNote Çoban F (December 1, 2024) Optimizing Sodium Azide (NaN₃) Mutagenesis for Cumin (Cuminum cyminum L.) Improvement. Selcuk Journal of Agriculture and Food Sciences 38 3 427–433.

Selcuk Agricultural and Food Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY NC).