Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology

Mahmut Kaplan; Serap Köprü; Rabia Say; Kevser Karaman; M. Mücahit Yılmaz

Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology

Abstract

Hypericum perforatum is a widely used medicinal plant known that it has high bioactive performance due to its special biochemical compounds in its structure. The current study aimed to determine the optimum processing parameters of extraction conditions of bioactive constituents in H. perforatum by ultrasound application and its some bioactive performance. For this purpose, different processing variables namely sonication period (min), liquid/solid ratio and ethanol concentration (%) were selected to characterize the best conditions for extraction of H. perforatum. The statistical analysis results revealed that the most effective factor on the studied parameters was ethanol concentration and increment of ethanol level increased the characterized responses until a constant value. The constructed regression models fit very well with quite high determination of coefficients (R2>0.906). Total phenolic content was in the range of 39.54-63.55 mg GAE/g sample while total hypericin concentration ranged between 211.5-858.7 mg/kg. Multiple response optimization results revealed that the optimum extraction conditions would be at 47.6% ethanol concentration and 22.2 mL of liquid/solid ratio for 60 min ultrasonic process application. The results revealed that the optimized extraction conditions could be utilized for ultrasound extraction system by industrial application.

Keywords

References

The article references can be accessed from the .pdf file.

Details

Primary Language

English

Subjects

Industrial Biotechnology

Journal Section

Research Article

Authors

Mahmut Kaplan This is me
0000-0002-6717-4115
Türkiye

Serap Köprü This is me
0000-0001-7706-238X
Türkiye

Rabia Say This is me
0000-0003-1868-3957
Türkiye

Kevser Karaman ^* This is me
0000-0003-0729-6185
Türkiye

M. Mücahit Yılmaz This is me
0000-0002-6338-4449
Türkiye

Publication Date

November 17, 2021

Submission Date

October 19, 2020

Acceptance Date

February 8, 2021

Published in Issue

Year 2021 Volume: 39 Number: 4

IZ

https://izlik.org/JA92YJ32KE

Cite

RIS / Bibtex

APA

Kaplan, M., Köprü, S., Say, R., Karaman, K., & Yılmaz, M. M. (2021). Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology. Sigma Journal of Engineering and Natural Sciences, 39(4), 392-403. https://izlik.org/JA92YJ32KE

AMA

1.Kaplan M, Köprü S, Say R, Karaman K, Yılmaz MM. Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology. SIGMA. 2021;39(4):392-403. https://izlik.org/JA92YJ32KE

Chicago

Kaplan, Mahmut, Serap Köprü, Rabia Say, Kevser Karaman, and M. Mücahit Yılmaz. 2021. “Characterization of in Vitro Bioactive Performance of Hypericum Perforatum Using Response Surface Methodology”. Sigma Journal of Engineering and Natural Sciences 39 (4): 392-403. https://izlik.org/JA92YJ32KE.

EndNote

Kaplan M, Köprü S, Say R, Karaman K, Yılmaz MM (November 1, 2021) Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology. Sigma Journal of Engineering and Natural Sciences 39 4 392–403.

IEEE

[1]M. Kaplan, S. Köprü, R. Say, K. Karaman, and M. M. Yılmaz, “Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology”, SIGMA, vol. 39, no. 4, pp. 392–403, Nov. 2021, [Online]. Available: https://izlik.org/JA92YJ32KE

ISNAD

Kaplan, Mahmut - Köprü, Serap - Say, Rabia - Karaman, Kevser - Yılmaz, M. Mücahit. “Characterization of in Vitro Bioactive Performance of Hypericum Perforatum Using Response Surface Methodology”. Sigma Journal of Engineering and Natural Sciences 39/4 (November 1, 2021): 392-403. https://izlik.org/JA92YJ32KE.

JAMA

1.Kaplan M, Köprü S, Say R, Karaman K, Yılmaz MM. Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology. SIGMA. 2021;39:392–403.

MLA

Kaplan, Mahmut, et al. “Characterization of in Vitro Bioactive Performance of Hypericum Perforatum Using Response Surface Methodology”. Sigma Journal of Engineering and Natural Sciences, vol. 39, no. 4, Nov. 2021, pp. 392-03, https://izlik.org/JA92YJ32KE.

Vancouver

1.Mahmut Kaplan, Serap Köprü, Rabia Say, Kevser Karaman, M. Mücahit Yılmaz. Characterization of in vitro bioactive performance of Hypericum perforatum using response surface methodology. SIGMA [Internet]. 2021 Nov. 1;39(4):392-403. Available from: https://izlik.org/JA92YJ32KE