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Optimization of ultrasound-assisted extraction of protein from the byproduct of the hazelnut oil industry using reverse micelles

Year 2024, Volume: 42 Issue: 4, 1202 - 1213, 01.08.2024

Abstract

In the current research, an ultrasound-assisted extraction (UAE) procedure was established for the protein extracted employing a reverse micelles system (RMS) from the by-product of the hazelnut oil industry. The optimum extraction circumstances in the UAE were identified as a dioctyl sodium sulfosuccinate (AOT) an amount of 0.05 g/mL, a water content of 25.2
(W0), a 0.02 g/mL solid-to-liquid ratio, ultrasound time of 17.52 min, ultrasound cycle 1, and ultrasound power 80% using the response surface approach. Under ideal circumstances, the maximum yield was recorded as 44.84 mg BSA/g of hazelnut meal protein (HMP) for RMS, it had a higher yield obtained by alkaline solution (AS). α-helix, β-turn, and β-sheet structures of HMP increased while the random coil decreased as evidenced by FTIR and SEM images proving that the cell walls were destructed and had more cracks in RMS. Overall, the find-ings indicated that UAE combined with RMS might be an effective approach for extracting protein from HMP and is likely to lead to an alternative evaluation possibility of an industrial by-product.

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There are 44 citations in total.

Details

Primary Language English
Subjects Clinical Chemistry
Journal Section Research Articles
Authors

Elif Meltem İşçimen 0000-0002-9849-6352

Mehmet Hayta 0000-0001-6239-8630

Publication Date August 1, 2024
Submission Date January 25, 2023
Published in Issue Year 2024 Volume: 42 Issue: 4

Cite

Vancouver İşçimen EM, Hayta M. Optimization of ultrasound-assisted extraction of protein from the byproduct of the hazelnut oil industry using reverse micelles. SIGMA. 2024;42(4):1202-13.

IMPORTANT NOTE: JOURNAL SUBMISSION LINK https://eds.yildiz.edu.tr/sigma/